Pan: a transcriptional regulator that binds chymotrypsin, insulin, and AP-4 enhancer motifs.

Nelson, Christine; Shen, Lu-Ping; Meister, Andreas; Fodor, E; Rutter, William J.

doi:10.1101/gad.4.6.1035

Cited by 191 publications

(117 citation statements)

References 26 publications

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“…Also, the cardiacspecific GATA-4 factor that was shown to stimulate ANF and BNP gene activity (Grepin et al 1994) was inert in this system. Overexpression of the ubiquitous HLH factor, rPanl (rodent homolog of E12) (Nelson et al 1990), did not activate transcription from the CE3 reporter but did activate transcription from a reporter construct containing the CElB E box of the POMC promoter (Fig. 4C).…”

Section: Fold Activationmentioning

confidence: 99%

Ptx1, a bicoid-related homeo box transcription factor involved in transcription of the pro-opiomelanocortin gene.

Lamonerie¹,

Tremblay²,

Lanctôt³

et al. 1996

Genes Dev.

394

305

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The pituitary gland contains six distinct hormone-producing cell types that arise sequentially during organogenesis. The first cells to differentiate are those that express the pro-opiomelanocortin (POMC) gene in the anterior pituitary lobe. The other lineages, which appear later, include cells that are dependent on the POU factor Pit-1 and another POMC-expressing lineage in the intermediate pituitary lobe. Using AtT-20 cells as a model for early expression of POMC in the anterior pituitary, we have defined a regulatory element conferring cell specificity of transcription and cloned a cognate transcription factor. This factor, Ptx1 (pituitary homeo box 1), contains a homeo box related to those of the anterior-specific genes bicoid and orthodenticle in Drosophila, and Otx-1 and Otx-2 in mammals. Ptx1 activates transcription upon binding a sequence related to the Drosophila bicoid target sites. Ptx1 is the only nuclear factor of this DNA-binding specificity that is detected in AtT-20 cells, and it is expressed at high levels in a subset of adult anterior pituitary cells that express POMC. However, Ptx1 is expressed in most cells of Rathke's pouch at an early time during pituitary development and before final differentiation of hormone-producing cells. Thus, Ptx1 may have a role in differentiation of pituitary cells, and its early expression pattern suggests that it may have a role in pituitary formation. In the adult pituitary gland, Ptx1 appears to be recruited for cell-specific transcription of the POMC gene.

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Section: Fold Activationmentioning

confidence: 99%

Ptx1, a bicoid-related homeo box transcription factor involved in transcription of the pro-opiomelanocortin gene.

Lamonerie¹,

Tremblay²,

Lanctôt³

et al. 1996

Genes Dev.

394

305

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“…Initially, the E-box binding complex was named IEF-1 (insulin enhancer factor-1) and thought only to be present in islet-cell lines (Ohlsson et al, 1988). It was later characterized as a heterodimeric complex, consisting of the ubiquitous E2A/Pan-factor (Nelson et al, 1990) and the tissuespecific Beta-2/NeuroD factor (Naya et al, 1995;Lee et al, 1995). NeuroD is expressed by several neuronal cell types, where it plays a role in inducing the neuronal program.…”

Section: Neurodmentioning

confidence: 99%

Gene regulatory factors in pancreatic development

Jensen

2003

Developmental Dynamics

347

289

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The intensity of research on pancreatic development has increased markedly in the past 5 years, primarily for two reasons: we now know that the insulin-producing ␤-cells normally arise from an endodermally derived, pancreasspecified precursor cell, and successful transplants of islet cells have been performed, relieving patients with type I diabetes of symptoms for extended periods after transplantation. Combining in vitro ␤-cell formation from a pancreatic biopsy of a diabetic patient or from other stem-cell sources followed by endocrine cell transplantation may be the most beneficial route for a future diabetes therapy. However, to achieve this, a thorough understanding of the genetic components regulating the development of ␤-cells is required. The following review discusses our current understanding of the transcription factor networks necessary for pancreatic development and how several genetic interactions coming into play at the earliest stages of endodermal development gradually help to build the pancreatic organ.

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“…The deduced amino acid sequence for mouse E47 shows high similarity with the previously described partial amino acid sequences of the mouse E47 protein (amino acids 1-153 and 323-478) (45) with the unique exception of the absence of residue Gln-387, which is also absent in the deduced amino acid sequences of the human (23, 24) and rat (46) E12/E47 cDNAs. Other specific changes detected in the amino acid sequence of the full mouse E47 protein were the insertion of two Ala residues (Ala-311, Ala-312), the absence of Ala-168, present in the rat E47 isoform (46), and the lack of several amino acid insertions uniquely present in the human E12/E47 proteins (GSSS at 260, Ala-295, His-510) (23,24).…”

Section: Mouse Bhlh Factor E47 Interacts With the E-pal Elementmentioning

confidence: 99%

A New Role for E12/E47 in the Repression ofE-cadherin Expression and Epithelial-Mesenchymal Transitions

Pérez-Moreno¹,

Locascio²,

Rodrigo³

et al. 2001

Journal of Biological Chemistry

405

338

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Down-regulation of E-cadherin expression is a determinant of tumor cell invasiveness, an event frequently associated with epithelial-mesenchymal transitions.Here we show that the mouse E12/E47 basic helix-loophelix transcription factor (the E2A gene product) acts as a repressor of E-cadherin expression and triggers epithelial-mesenchymal transitions. The mouse E47 factor was isolated in a one-hybrid system designed to isolate repressors of the mouse E-cadherin promoter. Epithelial cells ectopically expressing E47 adopt a fibroblastic phenotype and acquire tumorigenic and migratory/invasive properties, concomitant with the suppression of E-cadherin expression. Suppression of E-cadherin expression under stable or inducible expression of E47 in epithelial cells occurs at the transcriptional level and is dependent on the E-boxes of the E-cadherin promoter. Interestingly, analysis of endogenous E2A expression in murine and human cell lines illustrated its presence in E-cadherin-deficient, invasive carcinoma cells but its absence from epithelial cell lines. This expression pattern is consistent with that observed in early mouse embryos, where E2A mRNA is absent from epithelia but strongly expressed in the mesoderm. These results implicate E12/ E47 as a repressor of E-cadherin expression during both development and tumor progression and indicate its involvement in the acquisition and/or maintenance of the mesenchymal phenotype.

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Pan: a transcriptional regulator that binds chymotrypsin, insulin, and AP-4 enhancer motifs.

Cited by 191 publications

References 26 publications

Ptx1, a bicoid-related homeo box transcription factor involved in transcription of the pro-opiomelanocortin gene.

Ptx1, a bicoid-related homeo box transcription factor involved in transcription of the pro-opiomelanocortin gene.

Gene regulatory factors in pancreatic development

A New Role for E12/E47 in the Repression ofE-cadherin Expression and Epithelial-Mesenchymal Transitions

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