PAMP-induced peptide 1 cooperates with salicylic acid to regulate stomatal immunity in <i>Arabidopsis thaliana</i>

Hou, Sen; Shen, Hexi; Shao, Hongwei

doi:10.1080/15592324.2019.1666657

Cited by 18 publications

(26 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RLK7 belongs to the category XI of RLKs and acts as a receptor for PIP1 secreted peptide. Elevated expression of PIP1 has been described in Arabidopsis guard cells during Pseudomonas -induced PTI, and thus the peptide plays a role in stomatal immunity [ 72 ]. Moreover, RLK7 also contributes to Arabidopsis resistance to B. cinerea [ 73 ] and Phytium irregulare [ 74 ].…”

Section: Discussionmentioning

confidence: 99%

Complexity of Brassica oleracea–Alternaria brassicicola Susceptible Interaction Reveals Downregulation of Photosynthesis at Ultrastructural, Transcriptional, and Physiological Levels

Macioszek

Gapińska

Żmieńko

et al. 2020

Cells

View full text Add to dashboard Cite

Black spot disease, caused by Alternaria brassicicola in Brassica species, is one of the most devastating diseases all over the world, especially since there is no known fully resistant Brassica cultivar. In this study, the visualization of black spot disease development on Brassica oleracea var. capitata f. alba (white cabbage) leaves and subsequent ultrastructural, molecular and physiological investigations were conducted. Inter- and intracellular hyphae growth within leaf tissues led to the loss of host cell integrity and various levels of organelle disintegration. Severe symptoms of chloroplast damage included the degeneration of chloroplast envelope and grana, and the loss of electron denseness by stroma at the advanced stage of infection. Transcriptional profiling of infected leaves revealed that photosynthesis was the most negatively regulated biological process. However, in infected leaves, chlorophyll and carotenoid content did not decrease until 48 hpi, and several chlorophyll a fluorescence parameters, such as photosystem II quantum yield (Fv/Fm), non-photochemical quenching (NPQ), or plant vitality parameter (Rdf) decreased significantly at 24 and 48 hpi compared to control leaves. Our results indicate that the initial stages of interaction between B. oleracea and A. brassicicola are not uniform within an inoculation site and show a complexity of host responses and fungal attempts to overcome host cell defense mechanisms. The downregulation of photosynthesis at the early stage of this susceptible interaction suggests that it may be a part of a host defense strategy, or, alternatively, that chloroplasts are targets for the unknown virulence factor(s) of A. brassicicola. However, the observed decrease of photosynthetic efficiency at the later stages of infection is a result of the fungus-induced necrotic lesion expansion.

show abstract

Section: Discussionmentioning

confidence: 99%

Complexity of Brassica oleracea–Alternaria brassicicola Susceptible Interaction Reveals Downregulation of Photosynthesis at Ultrastructural, Transcriptional, and Physiological Levels

Macioszek

Gapińska

Żmieńko

et al. 2020

Cells

View full text Add to dashboard Cite

show abstract

“…The research has shown that PIP1-induced stomatal closure is RLK7 dependent ( Hou et al, 2019 ). To explore the mechanism of PIP1-RLK7 pathway in the regulation of stomatal response, we also conducted patch clamp whole-cell recordings of S-type anion currents in Col-0 and rlk7 guard cell protoplasts under control condition and treated with 10 µM PIP1.…”

Section: Resultsmentioning

confidence: 99%

“…We used two wild type ecotypes of A. thaliana in this study: Columbia-0 (Col-0) and Landsberg-0 (Ler-0). Col-0 was the background of PIP1 over-expression lines (PIP1-OE and PIP1-OE2), and mutants rlk7 ( rlk7-2 , SALK_083114) ( Hou et al, 2019 ), bak1 ( bak1-4 , SALK_116202) ( Hou et al, 2014 ), bik1 ( Zhang et al, 2010 ; Hou et al, 2014 ), mpk3/mpk6 (SALK_151594/SALK_073907) ( Xu et al, 2014 ; Zhang et al, 2019 ), slac1-1 , slac1-3 , and rbohD/F (CS9558) ( Shen et al, 2017 ). But ost1-1 and ost1-2 were Ler-0 ecotype background.…”

Section: Methodsmentioning

confidence: 99%

“…Arabidopsis secreted peptide PIP1 can be recognized by RLK7, which is plasma membrane-localized LRR-RLK ( Pitorre et al, 2010 ), and can amplify immunity response ( Hou et al, 2014 ). A recent report indicates that PIP1 cooperates with salicylic acid to regulate stomatal immunity in Arabidopsis thaliana ( Hou et al, 2019 ). In this study, we further confirmed that PIP1 could participate in the induction of stomatal closure.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Secreted Peptide PIP1 Induces Stomatal Closure by Activation of Guard Cell Anion Channels in Arabidopsis

et al. 2020

View full text Add to dashboard Cite

Plant stomata which consist of a pair of guard cells, are not only finely controlled to balance water loss as transpiration and CO 2 absorption for photosynthesis, but also serve as the major sites to defend against pathogen attack, thus allowing plants to respond appropriately to abiotic and biotic stress conditions. The regulatory signaling network for stomatal movement is complex in nature, and plant peptides have been shown to be involved in signaling processes. Arabidopsis secreted peptide PIP1 was previously identified as an endogenous elicitor, which induced immune response through its receptor, RLK7. PIP1-RLK7 can activate stomatal immunity against the bacterial strain Pst DC3118. However, the molecular mechanism of PIP1 in stomatal regulation is still unclear and additional new factors need to be discovered. In this study, we further clarified that PIP1 could function as an important regulator in the induction of stomatal closure. The results showed that PIP1 could promote stomata to close in a certain range of concentrations and response time. In addition, we uncovered that PIP1-RLK7 signaling regulated stomatal response by activating S-type anion channel SLAC1. PIP1-induced stomatal closure was impaired in bak1 , mpk3 , and mpk6 mutants, indicating that BAK1 and MPK3/MPK6 were required for PIP1-regulated stomatal movement. Our research further deciphered that OST1 which acts as an essential ABA-signaling component, also played a role in PIP1-induced stomatal closure. In addition, ROS participated in PIP1-induced stomatal closure and PIP1 could activate Ca 2+ permeable channels. In conclusion, we reveal the role of peptide PIP1 in triggering stomatal closure and the possible mechanism of PIP1 in the regulation of stomatal apertures. Our findings improve the understanding of the role of PIP1 in stomatal regulation and immune response.

show abstract

“…RLK7 belongs to category XI of RLKs and acts as a receptor for PIP1 secreted peptide. Elevated expression of PIP1 has been described in Arabidopsis guard cells during Pseudomonas-induced PTI, and thus the peptide plays a role in stomatal immunity [60]. Moreover, RLK7 also contributes to Arabidopsis resistance to B. cinerea [61] and Phytium irregulare [62].…”

Section: Defense-related Genes Are Activated Also In Susceptible Intementioning

confidence: 99%

Complexity of Brassica Oleracea-Alternaria Brassicicola Susceptible Interaction Reveals Down-regulation of Photosynthesis at Ultrastructural, Transcriptional and Physiological Levels

Macioszek

Gapińska

Żmieńko

et al. 2020

Preprint

View full text Add to dashboard Cite

BackgroundBlack spot disease, caused by Alternaria brassicicola in Brassica species, is one of the most devastating diseases all over the world, especially since there is no known fully resistant Brassica cultivar. In this study, the visualization of black spot disease development on B. oleracea var. capitata f. alba (white cabbage) leaves and subsequent ultrastructural, molecular and physiological investigations were conducted. ResultsFollowing germination on B. oleracea leaves, A. brassicicola invaded the host epidermal cells through appressoria, stomata or directly. The fungal attempts to penetrate epidermal cells usually evoked host cell death, and defense reactions manifested by the bright 'halo' around penetration site and host cell wall fortification were also observed. Inter- and intracellular hyphae growth within leaf tissues led to the loss of host cell integrity and various levels of organelle disintegration. Severe symptoms of chloroplast damage included the degeneration of chloroplast envelope and grana, and the loss of electron denseness by stroma at the advanced stage of infection. Transcriptional profiling of infected leaves revealed that photosynthesis was the most negatively regulated biological process. The down-regulation of 6 photosynthesis-related genes involved in light reactions and the Calvin cycle began as early as 12 hours post-inoculation (hpi) and extended to 44 genes at 48 hpi. Although in infected leaves, chlorophyll and carotenoid content did not decrease until 48 hpi, several chlorophyll a fluorescence parameters, such as photosystem II quantum yield (Fv/Fm), non-photochemical quenching (NPQ) or plant vitality parameter (Rdf) decreased significantly at 24 and 48 hpi compared to control leaves. ConclusionsOur results indicate that the initial stages of interaction between B. oleracea and A. brassicicola are not uniform within an inoculation site and show a complexity of host responses and fungal attempts to overcome host cell defense mechanisms. The down-regulation of photosynthesis at the early stage of this susceptible interaction suggests that it may be a part of a host defense strategy, or alternatively, that chloroplasts are targets for the unknown virulence factor(s) of A. brassicicola. However, the observed decrease of photosynthetic efficiency at the later stages of infection is a result of the fungus-induced necrotic lesion expansion.

show abstract

PAMP-induced peptide 1 cooperates with salicylic acid to regulate stomatal immunity in Arabidopsis thaliana

Cited by 18 publications

References 14 publications

Complexity of Brassica oleracea–Alternaria brassicicola Susceptible Interaction Reveals Downregulation of Photosynthesis at Ultrastructural, Transcriptional, and Physiological Levels

Complexity of Brassica oleracea–Alternaria brassicicola Susceptible Interaction Reveals Downregulation of Photosynthesis at Ultrastructural, Transcriptional, and Physiological Levels

Secreted Peptide PIP1 Induces Stomatal Closure by Activation of Guard Cell Anion Channels in Arabidopsis

Complexity of Brassica Oleracea-Alternaria Brassicicola Susceptible Interaction Reveals Down-regulation of Photosynthesis at Ultrastructural, Transcriptional and Physiological Levels

Contact Info

Product

Resources

About