Palytoxin toxicity after acute oral administration in mice

Sosa, Silvio; Favero, Giorgia Del; Bortoli, Marco; Vita, Francesca; Soranzo, Maria Rosa; Beltramo, D.; Ardizzone, Michele; Tubaro, Aurelia

doi:10.1016/j.toxlet.2009.09.009

Cited by 51 publications

(57 citation statements)

References 29 publications

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“…The mouse model experiment was designed according to the method of Sosa et al [7] with some modifications. Thirty ICR mice were divided into five groups randomly and fasted overnight (12 h) before treatment.…”

Section: Animalsmentioning

confidence: 99%

Investigation of the hazardous substance causing crayfish-induced rhabdomyolysis via a mouse model, a hemolysis assay, and a cytotoxicity assay

et al. 2015

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Section: Animalsmentioning

confidence: 99%

Investigation of the hazardous substance causing crayfish-induced rhabdomyolysis via a mouse model, a hemolysis assay, and a cytotoxicity assay

et al. 2015

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“…In view of the >90 structural analogues and the dearth of information as to their individual toxicities and thus lack of reliable teF values, eFSA employed a safety factor of 2 to allow for analogue differences, in addition to a safety factor of 100 to account for intra-and inter-species differences, finally arriving at an ARfD of 25 µg Ytxequiv./kg b.w. When employing the 400 g shellfish meat portion size and the currently permissible levels of Ytx of 1 mg Ytxequiv./kg shellfish meat, the resulting 651 µg/kg, (Munday, 2006;Sosa et al, 2009;tubaro et al, 2011a) in mice. Ostreocin-D exhibits effects similar to that of Pltx, but somewhat less severe at similar doses, after a single oral administration in mice (Ito and Yasumoto, 2009).…”

Section: Yessotoxin Shellfish Poisoning (Ysp)mentioning

confidence: 99%

A roadmap for hazard monitoring and risk assessment of marine biotoxins on the basis of chemical and biological test systems

Daneshian

2013

ALTEX

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Summary Aquatic food accounts for over 40% of global animal food products, and the potential contamination with toxins of algal origin -marine biotoxins -poses a health threat for consumers. The gold standards to assess toxins in aquatic food have traditionally been in vivo methodssingle marine biotoxins or combinations of marine biotoxins and intoxication symptomatology as well as monitoring the "success" of controls implemented during fishery and aquaculture production, processing, and retailing. For this reason, a workshop was organized in ermatingen, Switzerland by the Center for Alternatives to Animal testing -europe (CAAteurope) within the framework of the transatlantic think tank for toxicology (t 4 ).In contrast to other food products where hygiene and potential contamination with microbes or mold toxins is the prime issue, the emphasis in aquatic products (shellfish and finfish) is, beyond viral and bacterial contaminations, primarily placed on potential contamination with marine biotoxins owing to the numerous and recurring human intoxications.The gold standards to assess toxins in aquatic food have traditionally been in vivo methods, i.e., the mouse and the rat bioassays. Besides the ethical issues of in vivo bioassays there are specific difficulties, e.g., different exposure route (i.p. versus oral), low inter-species comparability, high intra-species variability, and questionable extrapolation of quantitative risk to humans, thus highlighting the need for the development of more reliable detection and quantification methods. Based on this reasoning, the european Food Safety Authority (eFSA) advocates the use of an analytical method, i.e., LC-MS (Liquid Chromatography coupled Mass Spectrometry), as a substitute for in vivo bioassays for almost all classes of marine toxins. However, although lC-MS is a very sensitive method that has the advantages of being able to detect multiple toxins in a single analysis and being good for confirming the identity of toxins, the quality of quantitative analysis is dependent on the availability of calibration standards. While many new toxin structural analogues have been detected and identified using LC-MS, this technique -as with most other methods -is not good at detecting new toxin types. When new toxin analogues are detected but accurate standards are not yet available, only an approximate quantitation is possible using estimated response factors.For the purpose of risk assessment of food, however, it is imperative to focus on the possible adverse effects, independent of whether they stem from one toxin or a combination of toxins. As toxicity is defined by functional and structural changes of biological systems, the development of a human-relevant in vitro system for appropriate risk assessment of marine biotoxins in seafood stands to reason. Moreover, poor correlation of human intoxications, of acute symptomatology and long-term adverse effects, and of predictive in vitro, in vivo, and analytical detection methodologies of marine biotoxins stems not least from a...

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“…histological and hematoclinical analyses). The toxicity was initially evaluated, within 24 h after the administration, on the parent compound PLTX and it was found to be strictly dose-related [70]. Later, the study was repeated on 42-hydroxy-PLTX [71], chemically characterized in 2009 [72] (Figure 2).…”

Section: Potentialexposuretoostreopsissppand Relatedtoxinsviaseafoodmentioning

confidence: 99%

“…Histological analysis revealed decreased glycogen content in hepatocytes. Mice that survived the treatment exhibited several degrees of inflammation of the mucosa in the forestomach [70,71].…”

Section: Potentialexposuretoostreopsissppand Relatedtoxinsviaseafoodmentioning

confidence: 99%

“…In animals treated from the dose of 600 µg/kg, hematochemical analysis revealed alteration in plasma levels of creatine phosphokinase (CPK), lactate dehydrogenase (LDH) and aspartate transaminase (AST), suggesting involvement of the muscular tissue in the toxicity of PLTX and 42-hydroxy-PLTX [70,71]. In animals treated with PLTX, dose-dependent ultrastructural alterations of skeletal and cardiac muscle were also observed.…”

Section: Potentialexposuretoostreopsissppand Relatedtoxinsviaseafoodmentioning

confidence: 99%

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Sanitary problems related to the presence of Ostreopsis spp. in the Mediterranean Sea: a multidisciplinary scientific approach

Favero

Sosa

Pelin

et al. 2012

Annali Dell'Istituto Superiore Di Sanità

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Abstract. The increased presence of potentially toxic microalgae in the Mediterranean area is a matter of great concern. Since the end of the last century, microalgae of the genus Ostreopsis have been detected more and more frequently in the Italian coastal waters. The presence of Ostreopsis spp. has been accompanied by the presence of previously undetected marine biotoxins (palytoxins) into the ecosystem with the increased possibility of human exposure. In response to the urgent need for toxicity characterization of palytoxin and its congeners, an integrated study encompassing both in vitro and in vivo methods was performed.

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Palytoxin toxicity after acute oral administration in mice

Cited by 51 publications

References 29 publications

Investigation of the hazardous substance causing crayfish-induced rhabdomyolysis via a mouse model, a hemolysis assay, and a cytotoxicity assay

Investigation of the hazardous substance causing crayfish-induced rhabdomyolysis via a mouse model, a hemolysis assay, and a cytotoxicity assay

A roadmap for hazard monitoring and risk assessment of marine biotoxins on the basis of chemical and biological test systems

Sanitary problems related to the presence of Ostreopsis spp. in the Mediterranean Sea: a multidisciplinary scientific approach

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