Palyno‐morphological investigations of subtropical endangered flora of Capparidaceae through light and scanning electron microscopy

et al. 2022

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Palynological characterization is considered to be one of the significant taxonomic tools for the delimitation and identification of morphologically complicated taxa.Hence, the pollen morphology of 12 species of spineless Astragalus L. was examined using light and scanning electron microscopy. Studied pollen were small to medium, monad, prolate to per-prolate and tricolporate type in all studied taxa. The exine sculpturing varied from reticulate to microreticulate whereas colpus ornamentation ranged from scabrate to granulate. Furthermore, maximum polar and equatorial diameter was recorded in Astragalus leucocephalus Bunge. (45.00 μm) and A. pyrrhotrichus Boiss. (22.91 μm) while minimum in A. amherstianus Benth. ex Royle (28.75 μm) and A. amherstianus Benth. ex Royle (15.00 μm), respectively. Similarly, the ratio of polar to equatorial diameter was recorded maximum in A. ophiocarpus Boiss. (2.05). The width of colpi was larger in A. hamosus L. (1.29 μm) and smaller in A. ophiocarpus Boiss. (0.62 μm). We have also found the maximum value of mesocolpium in A. retamocarpus Boiss. (2.08 μm) while minimum in A. oxyglottis Steven ex M.Bieb.(1.87 μm). The quantitative pollen attributes helped in the development of pollen keys for the accurate and quick identification of the studied species. Furthermore, ordination and cluster analysis were performed for the differentiation of the investigated taxa at species level. Based on our results, we conclude that pollen features can be used for the delimitation and identification of the studied taxa. Research Highlights• Pollen micromorphology is a useful tool for classifying complicated taxa.• The pollen micromorphology of 12 spineless species of Astragalus L. was studied using LM and SEM.• The observed pollen characteristics aided in Astragalus L. serve for the identification and classification of taxa at specific level.

Section: Scanning Electron Microscopymentioning

confidence: 99%

Light and scanning electron microscopic observation of palynological characteristics in spinelessAstragalusL. (Fabaceae) and its taxonomic significance

Khan

et al. 2022

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“…Morphology of pollen is of massive importance for the interpretation and documentation of relationship among different species at several different taxonomic levels (Khan et al, 2018). Palynologist investigated the significance of palynological features in different group of plants from time to time and inaugurate it highly effective in the process of identification of plant species (Ashfaq et al, 2020; Jamil Noor et al, 2016; Khan et al, 2018; Mir et al, 2019; Noor et al, 2017). Palynology of medicinally important plants has been carried out in Pakistan which inspected pollen morphology and medicinal properties of these plants (Qureshi et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Palyno‐morphological diversity of Asteraceous and Poaceous allergenic plant using microscopic techniques in lesser Himalaya‐Pakistan

Anar

et al. 2022

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In classification and identification of plant species, palyno morphological character has remain helpful for plant taxonomist. The aim to investigation this study was to identify the characteristic features of Asteraceae and Poaceae family member collected from lower Himalayas of Pakistan. Light microscope and Scanning electron microscope were used for feature study of allergenic pollen. Our study includes 10 species of Asteraceae and Poaceae family. The most dominant shape of pollen was Oblate-spheroidal, Prolate-Spheroidal, Prolate and Suboblate shape of pollen.Quantitative character of pollen includes maximum pollen diameter found in Dichanthium annulatum (46.0 μm) and minimum polar axis pollen diameter found in Parthenium hysterophorous (16.0 μm). Similarly highest value of P/E ratio was noted in Cenchrus echinatus (1.05) and smallest value of P/E ratio was observed in Erigeron bonariensis (0.88). Poaceae family member were monoporate. Character feature of exine sculpturing include Microechinate, granulate, echinate, conical base, lacunae Nanogammate, Aerolate-Scabratre, Verrucate, like variation found in exine of both family member. Highest value of pollen fertility was observed in P. hysterophorous 93%, Similarly maximum value of pollen sterility was noted in E. bonariensis and Brachiaria reptans 24%. This investigation provides the characteristic feature of allergenic pollen species and help to arrange them in define class.

“…In 1945 Williams and Hyde proposed the word “palynology” for the first time (Mir et al, 2019). Palynology assists in correct identification and classification of the problematic taxa at species and generic level, and also have vital role in other field of science like mellisoplaynology, aeropalynology, and pharmacopalynology (Gul et al, 2021; Khan et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…Various palynological features such as pollen size, ornamentation, shape, aperture, and sculpturing are important for the correct identification of plants species (Salamma, Kailas, Naik, Rao, & Hari, 2019). Pollen fertility and sterility percentages give us helpful information regarding penitential hybrids (Mir et al, 2019). The pollen fertility data for any plant species is vital for commercial scaled growers and plant breeders (Rigamoto & Tyagi, 2002).…”

Section: Introductionmentioning

confidence: 99%

Palyno‐anatomical characters and their systematic significance in the family Apiaceae from Chitral, eastern Hindu Kush, Pakistan

Birjees

et al. 2021

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The present study was performed to provide a detailed explanation of leaf epidermal anatomy and pollen micromorphological features of selected species of family Apiaceae from Chitral, eastern Hindu Kush region as the basis of forthcoming studies.In the present article pollen morphology of eight species and foliar epidermal of seven species of family Apiaceae have been examined through microscopic techniques. In results two types of pollen prolate (five species) and perprolate (three species) with three colpi have been recorded. The exine ornamentation was found to be regulate, striate, and cerebroid. Largest pollen was found in Heracleum leucocarpum with the polar diameter of 43.25 μm and equatorial diameter of 21.6 μm. Smallest pollen was observed in Elaeosticta chitralica with the polar diameter of 18.4 μm. The P/E ratio varied from 1.59 to 2.16. Regarding to foliar epidermal anatomy, three types of epidermal cells including rectangular, irregular, and polygonal with variation in anticlinal wall pattern were determined. In the selected species three kinds of stomata comprising anisocytic, anomocytic, and paracytic type were reported in the current research. The size of epidermal cells ranged from 106 Â 42.50 μm in Bupleurum falcatum subsp. cernuum and 77.25 Â 26.35 μm in Prangos pabularia in adaxial surface. Largest stomatal complex was found in Prangos pabularia both in adaxial 33.55 Â 20.05 μm and abaxial 50.25 Â 39.40 μm. All the observed quantitative and qualitative features of the species were proved to be useful in the delimitation of species at generic and species level.