Palmitoylation of CYSTM (CYSPD) proteins in yeast

Genome-wide conditional degron libraries for functional genomics

“…The relationship between terminal localization signals and differential fitness of N- and C-terminally tagged strains was examined using a list of 998 validated or predicted localization signals obtained through literature curation or retrieved from the UniProt database (Bateman et al, 2023) (Table S2). The list consists of proteins with a glycosylphosphatidylinositol (GPI) anchor (De Groot et al, 2003; Gíslason et al, 2021), tail-anchored (TA) proteins (Beilharz et al, 2003; Burri and Lithgow, 2004; Lewis and Pelham, 2002; Schuldiner et al, 2008; Šimočková et al, 2008), proteins with a cysteine-rich transmembrane module (CYSTM) (Giolito et al, 2024; Venancio and Aravind, 2010; Zvonarev et al, 2023), acylated proteins (UniProt as of 19.02.2024, search for proteins with lipidation, including N-myristoylation, palmitoylation, GPI-anchor addition and prenylation), proteins with a PTS1 peroxisomal targeting signal (Nötzel et al, 2016) (UniProt as of 24.04.2024, search for proteins with motif: microbody targeting signal, C-terminal peroxisome targeting signal (PTS1), peroxisome targeting signal (PTS1), SKL peroxisome targeting motif, peroxisomal target signal 1 (PTS1) or peroxisomal targeting signal type 1), proteins with a signal peptide (SP) or a mitochondrial targeting signal (MTS) (Somashekara and Muniyappa, 2022; Weill et al, 2018; Yofe et al, 2016), proteins with nuclear localization signals (Bordonné, 2000; Hahn et al, 2008; Lee et al, 2023; Somashekara and Muniyappa, 2022) and proteins with HDEL, KKXX or KXKXX motifs identified by pattern match or retrieved from Uniprot (UniProt as of 24.04.2024, search for proteins with motif: prevents secretion from ER or di-lysine motif).…”