Palmitic acid incorporation into intramuscular acylglycerols depends on both total and unbound to albumin palmitic acid concentration

Abstract

Palmitic acid incorporation into the intramuscular acylglycerols in rat skeletal muscles of different fiber types was investigated at various total and unbound to albumin concentrations by means of the hind-limb perfusion technique. It was found that at simultaneously increasing total and unbound to albumin palmitic acid concentrations in the perfusion medium the incorporation of palmitic acid into acylglycerols increased. However, when the concentration of palmitic acid not bound to albumin was kept constant and the total palmitic acid concentration was increased, the incorporation also increased although markedly less than under former conditions. The increase was most apparent in the muscles composed of slow-twitch oxidative and fast-twitch oxidative-glycolytic fibers where fatty acid uptake is the greatest. These findings suggest that fatty acid incorporation into intramuscular acylglycerols depends not only on the unbound to albumin fatty acid concentration but also, to some extent, on the total fatty acid concentration.

Non-esterified fatty acids in follicular fluid of dairy cows and their effect on developmental capacity of bovine oocytes in vitro

“…The importance of the albumin bound fatty acids in this process remains a matter of discussion. It does seem as though both forms of fatty acids are taken up by the cells, suggesting the physiological significance of the total NEFA concentration (McArthur et al 1999, Synak et al 2003. In preliminary experiments with fatty acid free albumin and with albumin bound OA, albumin itself exerted a negative effect on the oocyte's developmental competence (Leroy et al 2003).…”

Physiological and Pharmacological Regulation of Triacylglycerol Storage and Mobilization

“…11.1). Using the hindlimb perfusion technique it was demonstrated recently that FA incorporation into intramuscular TAG depends not only on the unbound to albumin FA concentration but also, to some extent, on the total FA concentration [19]. While the direct involvement of FA not bound to albumin in FA uptake by adipocytes is generally accepted [17], it is still debated whether, and if so to what extent, the albumin-bound FA pool is directly engaged in this process [18].…”