Palmitic Acid Curcumin Ester Facilitates Protection of Neuroblastoma against Oligomeric Aβ40 Insult

Abstract

Background/Aims: The generation of reactive oxygen species (ROS) caused by amyloid-β (Aβ) is considered to be one of mechanisms underlying the development of Alzheimer's disease. Curcumin can attenuate Aβ-induced neurotoxicity through ROS scavenging, but the protective effect of intracellular curcumin on neurocyte membranes against extracellular Aβ may be compromised. To address this issue, we synthesized a palmitic acid curcumin ester (P-curcumin) which can be cultivated on the cell membrane and investigated the neuroprotective effect of P-curcumin and its interaction with Aβ. Methods: P-curcumin was prepared through chemical synthesis. Its structure was determined via nuclear magnetic resonance (NMR) and highresolution mass spectrometry (HRMS). An MTT assay was used to assess Aβ cytotoxicity and the protective effect of P-curcumin on SH-SY5Y cells. The effect of P-curcumin on Aβ-induced ROS production in vitro and in vivo were assessed based on changes in dichlorofluorescein (DCF) fluorescence. A spectrophotometric method was employed to detect lipid peroxidation. To mimic the interaction of P-curcumin on cell membranes with Aβ, liposomes were prepared by thin film method. Finally, the interactions between free P-curcumin and P-curcumin cultivated on liposomes and Aβ were determined via spectrophotometry. Results: A novel derivative, palmitic acid curcumin ester was prepared and characterized. This curcumin, cultivated on the membranes of neurocytes, may prevent Aβ-mediated ROS production and may inhibit the direct interaction between Aβ and the cellular membrane. Furthermore, P-curcumin could scavenge Aβ-mediated ROS as curcumin in vitro and in vivo, and had the potential to prevent lipid peroxidation. Morphological analyses showed that P-curcumin was better than curcumin at protecting cell shape. To examine P-curcumin's ability to attenuate direct interaction between Aβ and cell membranes, the binding affinity of Aβ to curcumin and P-curcumin was determined. The association constants for free P-curcumin and curcumin were 7. M -1 and 7.61 × 10 5 M -1 , respectively. In the liposome-trapped state, the association constants were 3.71 × 10 5 M -1 for P-curcumin and 1.44× 10 6 M -1 for curcumin. With this data, the thermodynamic constants of P-curcumin association with soluble Aβ (ΔH, ΔS, and ΔG) were also determined. Conclusion: Cultivated curcumin weakened the direct interaction between Aβ and cell membranes and showed greater neuroprotective effects against Aβ insult than free curcumin.

Growth Inhibition of a Novel Iron Chelator, DpdtC, against Hepatoma Carcinoma Cell Lines Partly Attributed to Ferritinophagy‐Mediated Lysosomal ROS Generation

“…Lipid peroxidation analysis was performed based on spectrophotometry, in which the ferrous ion is oxidized by lipid hydroperoxides to the ferric ion and subsequently reacts with thiocyanate to form a colored complex [ 30 ]. The details were as previously described [ 48 ]. Briefly, the trypsinized cells were collected and treated with the DpdtC for 12 h; the supernatant was removed by centrifugation and washed with PBS.…”

Curcumin‑loaded PEG‑PDLLA nanoparticles for attenuating palmitate‑induced oxidative stress and cardiomyocyte apoptosis through AMPK pathway

“…CUR has been used as anti-inflammatory, antioxidant and anticancer agents over a long period of time for the treatments of a number of types of diseases, including varied types of cancers, cardiovascular diseases and autoimmune diseases (19-24). A new study reveals that curcumin has the ability to attenuate oxidative stress in the myocardium and can play a protective and therapeutic role in the protection of the myocardium against lipotoxic injury owing to its lipid-lowering properties (25). In another study involving diabetic rats, CUR shows the ability to reduce cardiomyocyte remodeling and relieve cardiac insufficiency (26).…”

Liposomal Curcumin Targeting Endometrial Cancer Through the NF-κB Pathway

“…Curcumin, a lipid-soluble active ingredient of curcuma in traditional Chinese medicine, has diverse pharmacological effects against inflammation, oxidation, bacteria, and tumors [22]. Because of its abundance, low cost, and availability, it has become a promising antitumor drug for further exploitation and application and has been widely applied to antitumor experiments worldwide, which have shown significant achievements in preventing the occurrence, development, and metastasis of tumors [7].…”