Palmatine antioxidant and anti-acetylcholinesterase activities: A pre-clinical assessment

Abstract

There is evidence that palmatine (PA), an alkaloid isolated from the Guatteria friesiana plant, has some important biological activities, including antiinflammatory and antidepressant effects. In this study, the antioxidant and anti-acetylcholinesterase (AChE) effects of PA were assessed. The antioxidant capacity was evaluated in vitro and in vivo through 7 distinct assays, and the anti-AChE activity was determined in vitro. The standards, trolox and ascorbic acid were used for the in vitro antioxidant test, while hydrogen peroxide was selected as a stressor for the Saccharomyces cerevisiae test. Additionally, PA was also combined with trolox and ascorbic acid to determine the likelihood of synergistic effects occurrence to what concerns to antioxidant potential. PA exhibited a potent and concentration-dependent antioxidant potential, although a stronger antioxidant activity was stated using the PA + trolox combination. PA was also found to inhibit AChE activity when compared to the negative control. Thus, PA may be viewed as a promissory phytotherapeutic agent to manage oxidative stress-mediated neurological diseases, especially the Alzheimer's and Parkinson's diseases.

Protective Effects of <i>Coptis chinensis</i> Rhizome Extract and Its Constituents (Berberine, Coptisine, and Palmatine) against α-Synuclein Neurotoxicity in Dopaminergic SH-SY5Y Cells

“…21) Palmatine also exhibits potent, concentration-dependent antioxidative activity, contributing to the management of oxidative stress-induced PD. 61) In the α-synuclein-expressing cells used in the present study, palmatine reduced α-synuclein aggregation and promoted neurite outgrowth by upregulating GBA expression and enhancing autophagy; to the best of our knowledge, the present study is the first to report this finding. Among the three examined constituents of C. chinensis rhizome extract, palmatine was most effective in reducing caspase-3 activity (Fig.…”

Berberis vulgaris L. Root Extract as a Multi-Target Chemopreventive Agent against Colon Cancer Causing Apoptosis in Human Colon Adenocarcinoma Cell Lines

“…El Khalki et al 2018 reported an EC50 value of 69.65 µg/mL for the root ethanolic extract and 77.75 µg/mL for the root acetone extract [22]. Based on the chromatographical results obtained, it can be concluded that this antioxidant potential is caused by alkaloids with established antioxidant activity, which were determined in this study in BVR: berberine [26], palmatine [27], jatrorrhizine [28], and magnoflorine [29]. However, it is probable that there are other metabolites with antioxidant activity that have not yet been identified in BVR, which requires further research.…”

<em>Berberis vulgaris</em> L. Root Extract as a Multi-target Chemopreventive Agent against Colon Cancer

“…El Khalki et al 2018 reported an EC50 value of 69.65 μg/mL for the root ethanolic extract and 77.75 μg/mL for the root acetone extract [22]. Based on the chromatographical results obtained, it can be concluded that the antioxidant potential is caused by alkaloids with established antioxidant activity and determined in this study in BVR: berberine [30], palmatine [31], jatrorrhizine [25], magnoflorine [32]. However, probable there are other metabolites with antioxidant activity that have not yet been identified in BVR, which requires further research.…”