Palladium(II) Complexes of Aminopolycarboxylic Ligands in Aqueous Solution

Stefano, Concetta De; Gianguzza, Antonio; Pettignano, Alberto; Sammartano, Silvio

doi:10.1021/je200759g

Cited by 12 publications

(21 citation statements)

References 42 publications

(84 reference statements)

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“…Conversely, the loss of antigen-binding specificity does not appear to be an issue when using monomeric BFCAs that have smaller molecular structures, e.g., isothiocyanobenzyl-EDTA (SCN-Bn-EDTA, molecular weight (MW) 439 Da) was successfully used to conjugate CD45 antibodies with palladium isotopic cations for barcoding assays of live cells 25 . A previous study of palladium complexes of aminopolycarboxylic ligands in aqueous solution reported that DTPA complex with Pd 2+ is more stable (log K of 36.31) than EDTA complex with Pd 2+ (log K of 23.60) 48 . Therefore, in this presented protocol, monomeric BFCA of maleimidefunctionalized-DOTA was used to chelate Pd 2+ isotopic cations and was conjugated to the monoclonal antibody, as illustrated in Fig.…”

Section: Comparison Of Metal Isotope Chelation Methodsmentioning

confidence: 94%

Metal-isotope-tagged monoclonal antibodies for high-dimensional mass cytometry

et al. 2018

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Advances in single-cell mass cytometry have increasingly improved highly multidimensional characterization of immune cell heterogeneity. The immunoassay multiplexing capacity relies on monoclonal antibodies labeled with stable heavy-metal isotopes. To date, a variety of rare-earth elements and noble and post-transition metal isotopes have been used in mass cytometry; nevertheless, the methods used for antibody conjugation differ because of the individual metal coordination chemistries and distinct stabilities of various metal cations. Herein, we provide three optimized protocols for conjugating monoclonal IgG antibodies with 48 high-purity heavy-metal isotopes: (i) 38 isotopes of lanthanides, 2 isotopes of indium, and 1 isotope of yttrium; (ii) 6 isotopes of palladium; and (iii) 1 isotope of bismuth. Bifunctional chelating agents containing coordinative ligands of monomeric DOTA (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid) or polymeric pentetic acid (DTPA) were used to stably sequester isotopic cations in aqueous solutions and were subsequently coupled to IgG antibodies using site-specific biorthogonal reactions. Furthermore, quantification methods based on antibody inherent absorption at 280 nm and on extrinsic absorption at 562 nm after staining with bicinchoninic acid (BCA) are reported to determine metal-isotope-tagged antibodies. In addition, a freeze-drying procedure to prepare Reprints and permissions information is available at www.nature.com/reprints.

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Section: Comparison Of Metal Isotope Chelation Methodsmentioning

confidence: 94%

Metal-isotope-tagged monoclonal antibodies for high-dimensional mass cytometry

et al. 2018

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“…Palladium concentration was checked by ICP-AES technique against Pd(NO 3 ) 2 standard solutions (Fluka) or gravimetrically using the dimethylglyoxime method. 7,8 NTA, EGTA, DTPA, HEDP, and DTPP were supplied by Fluka with analytical grade media. Both kind of measurements were done using an 809 Metrohm Titrando apparatus equipped with a combined Orion glass electrode Ross type 8102.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…In the last years our research group carried out a systematic study on the binding ability of several ligand classes having different kind of binding groups toward Pd 2+ ion and some stability data on palladium(II)−ligand complexes have already been published. 7,8 Among these ligands, five aminopolycarboxylates (APCs) having different number of amino and carboxylic groups were taken into account. The interest for this ligand class arises from their strong binding ability toward metal ions and for this reason these compounds find interesting applications in different cases in which the sequestration/ removal of some metal ions is requested.…”

Section: ■ Introductionmentioning

confidence: 99%

“…For this purpose, iodide (I − ) and ammonia (NH 3 ) were used and the stability of the various PdI k or Pd(NH 3 ) k species has been taken from the literature. 7,22 The speciation study of the palladium/ligand systems was made also by spectrophotometric technique in NaClO 4 medium, at different ionic strengths, in the temperature range 283.15 ≤T/K ≤ 318. 15 and was preceded by the potentiometric determination of the ligand protonation constants in NaNO 3 medium.…”

Section: ■ Introductionmentioning

confidence: 99%

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Sequestering Ability of Aminopolycarboxylic (APCs) and Aminopolyphosphonic (APPs) Ligands Toward Palladium(II) in Aqueous Solution

et al. 2014

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The binding capacity of three aminopolycarboxylates [nitrilotriacetic acid (NTA), ethylene-glycol-bis(2-aminoethyl ether)- N,N,N′,N′-tetraacetic acid (EGTA), and diethylenetriamine- N,N,N′,N″,N″-pentaacetic acid (DTPA)] and two aminopolyphosphonates {(1-hydroxyethane-1,1-diyl)bis(phosphonic acid) (HEDP) and [[(phosphonomethyl)imino]bis[2,1-ethanediylnitrilobis(methylene)]] tetrakis-phosphonic acid (DTPP)} toward palladium(II) ion was studied by potentiometric and spectrophotometric titrations at different temperatures (283.15 ≤ T/K ≤ 318.15) and ionic strengths (0.1 ≤ I/mol·dm–3 ≤ 1.0) in NaClO4. The hydrolysis of Pd2+ and the protonation of ligands were always taken into account in the speciation models of Pd2+/L systems investigated. Equilibrium reaching experiments were performed to check and confirm the reaching of the equilibrium state. Owing to the high stability of the PdL species (K > 1020), for EGTA, HEDP, and DTPP it was determined using exchange measurements with auxiliary ligands, such as iodide (I–) and ammonia (NH3). For the other ligands the stability of the PdL species was reported in the literature. The general speciation scheme consisted of mononuclear differently protonated species with general formula PdHiL and only in the case of the NTA ligand the formation of the PdL2 species was found. The stability of the PdL species is high: as an example we have log K ML = 17.82, 22.60, 36.31, 23.49, and 27.27 for NTA, EGTA, DTPA, HEDP, and DTPP, respectively. Among the ligands, DTPA shows the highest formation constants and sequestering ability, evaluated using the pL0.5 parameter, as well. The complex formation reaction is always exothermic and in general the entropic contribution to the stability is dominant. Some empirical relationships were found to model the dependence of the formation constants on the number of protons and of the sequestering ability on pH.

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“…In the last years, our research group undertook a systematic study on the sequestering ability of several APCs and APPs towards different metal ions of environmental and biological interest [13][14][15][16]. As a further contribution, a study on the interaction of Ag + with four APCs [nitrilotriacetic acid (NTA), ethylene-glycol-bis (2-aminoethylether Silver is a rare metal usually present in nature as mineral ore, and the most abundant is argentite.…”

Section: Introductionmentioning

confidence: 99%