Palladium(II) and Platinum(II) Bind Strongly to an Engineered Blue Copper Protein

McLaughlin, Matthew P.; Darrah, Thomas H.; Holland, Patrick L.

doi:10.1021/ic2017648

Cited by 22 publications

(10 citation statements)

References 57 publications

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“…During sample analysis sample lines were rinsed to reduce memory effects by washing sequentially with Milli-Q purified water for two minutes and 2% nitric acid for an additional two minutes between analyses. ICP-MS analyses were conducted as previously reported 43 , 44 . Throughout the analysis, CeO + /Ce + was<2.1%.…”

Section: Methodsmentioning

confidence: 99%

“…Calculated solution concentrations obtained from 140 Ce and 142 Ce varied by less than 3.1% on average. [Ce] quantification was obtained using a 7-point external calibration curve spiked with known quantities of Ce in a linear range from 0.050 ng g −1 to 100 ng g −1 43 , 44 . Known aliquots of Ce spikes were analyzed as unknowns to determine external precision as 2.7%.…”

Section: Methodsmentioning

confidence: 99%

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Estimating the effective density of engineered nanomaterials for in vitro dosimetry

et al. 2014

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The need for accurate in vitro dosimetry remains a major obstacle to the development of cost-effective toxicological screening methods for engineered nanomaterials. An important key to accurate in vitro dosimetry is the characterization of sedimentation and diffusion rates of nanoparticles suspended in culture media, which largely depend upon the effective density and diameter of formed agglomerates in suspension. Here we present a rapid and inexpensive method for accurately measuring the effective density of nano-agglomerates in suspension. This novel method is based on the volume of the pellet obtained by bench-top centrifugation of nanomaterial suspensions in a packed cell volume tube, and is validated against gold-standard analytical ultracentrifugation data. This simple and cost-effective method allows nanotoxicologists to correctly model nanoparticle transport, and thus attain accurate dosimetry in cell culture systems, which will greatly advance the development of reliable and efficient methods for toxicological testing and investigation of nano-bio interactions in vitro.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Estimating the effective density of engineered nanomaterials for in vitro dosimetry

et al. 2014

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“…Folate concentrations were measured using a commercial kit, ID-Vit Folic acid (Immundiagnostic-ALPCO; Salem, NH) [ 54 ]. Metal concentrations were measured as nanograms per gram ( ng / g ) using solution-based inductively coupled plasma mass spectrometry (ICP-MS) methods previously described [ 55 – 57 ]. All standards, including aliquots of the certified NIST 955c, and procedural blanks were prepared by the same process.…”

Section: Methodsmentioning

confidence: 99%

Maternal blood cadmium, lead and arsenic levels, nutrient combinations, and offspring birthweight

et al. 2017

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BackgroundCadmium (Cd), lead (Pb) and arsenic (As) are common environmental contaminants that have been associated with lower birthweight. Although some essential metals may mitigate exposure, data are inconsistent. This study sought to evaluate the relationship between toxic metals, nutrient combinations and birthweight among 275 mother-child pairs.MethodsNon-essential metals, Cd, Pb, As, and essential metals, iron (Fe), zinc (Zn), selenium (Se), copper (Cu), calcium (Ca), magnesium (Mg), and manganese (Mn) were measured in maternal whole blood obtained during the first trimester using inductively coupled plasma mass spectrometry. Folate concentrations were measured by microbial assay. Birthweight was obtained from medical records. We used quantile regression to evaluate the association between toxic metals and nutrients due to their underlying wedge-shaped relationship. Ordinary linear regression was used to evaluate associations between birth weight and toxic metals.ResultsAfter multivariate adjustment, the negative association between Pb or Cd and a combination of Fe, Se, Ca and folate was robust, persistent and dose-dependent (p < 0.05). However, a combination of Zn, Cu, Mn and Mg was positively associated with Pb and Cd levels. While prenatal blood Cd and Pb were also associated with lower birthweight. Fe, Se, Ca and folate did not modify these associations.ConclusionSmall sample size and cross-sectional design notwithstanding, the robust and persistent negative associations between some, but not all, nutrient combinations with these ubiquitous environmental contaminants suggest that only some recommended nutrient combinations may mitigate toxic metal exposure in chronically exposed populations. Larger longitudinal studies are required to confirm these findings.Electronic supplementary materialThe online version of this article (doi:10.1186/s12889-017-4225-8) contains supplementary material, which is available to authorized users.

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“…For the first 310 participants, one

aliquot was used to measure Cd using established solution-based inductively coupled plasma mass spectrometry (ICP-MS) methods that have been previously described ( Darrah et al. 2009 ; McLaughlin et al. 2011 ; Vidal et al.…”

Section: Methodsmentioning

confidence: 99%

Effects of Cadmium Exposure on DNA Methylation at Imprinting Control Regions and Genome-Wide in Mothers and Newborn Children

Cowley

Skaar

Jima

et al. 2018

Environ Health Perspect

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Background:Imprinted genes are defined by their preferential expression from one of the two parental alleles. This unique mode of gene expression is dependent on allele-specific DNA methylation profiles established at regulatory sequences called imprinting control regions (ICRs). These loci have been used as biosensors to study how environmental exposures affect methylation and transcription. However, a critical unanswered question is whether they are more, less, or equally sensitive to environmental stressors as the rest of the genome.Objectives:Using cadmium exposure in humans as a model, we aimed to determine the relative sensitivity of ICRs to perturbation of methylation compared to similar, nonimprinted loci in the genome.Methods:We assayed DNA methylation genome-wide using bisulfite sequencing of 19 newborn cord blood and 20 maternal blood samples selected on the basis of maternal blood cadmium levels. Differentially methylated regions (DMRs) associated with cadmium exposure were identified.Results:In newborn cord blood and maternal blood, 641 and 1,945 cadmium-associated DMRs were identified, respectively. DMRs were more common at the 15 maternally methylated ICRs than at similar nonimprinted loci in newborn cord blood (p=5.64×10−8) and maternal blood (p=6.22×10−14), suggesting a higher sensitivity for ICRs to cadmium. Genome-wide, Enrichr analysis indicated that the top three functional categories for genes that overlapped DMRs in maternal blood were body mass index (BMI) (p=2.0×10−5), blood pressure (p=3.8×10−5), and body weight (p=0.0014). In newborn cord blood, the top three functional categories were BMI, atrial fibrillation, and hypertension, although associations were not significant after correction for multiple testing (p=0.098). These findings suggest that epigenetic changes may contribute to the etiology of cadmium-associated diseases.Conclusions:We analyzed cord blood and maternal blood DNA methylation profiles genome-wide at nucleotide resolution in individuals selected for high and low blood cadmium levels in the first trimester. Our findings suggest that ICRs may be hot spots for perturbation by cadmium, motivating further study of these loci to investigate potential mechanisms of cadmium action. https://doi.org/10.1289/EHP2085

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Palladium(II) and Platinum(II) Bind Strongly to an Engineered Blue Copper Protein

Cited by 22 publications

References 57 publications

Estimating the effective density of engineered nanomaterials for in vitro dosimetry

Estimating the effective density of engineered nanomaterials for in vitro dosimetry

Maternal blood cadmium, lead and arsenic levels, nutrient combinations, and offspring birthweight

Effects of Cadmium Exposure on DNA Methylation at Imprinting Control Regions and Genome-Wide in Mothers and Newborn Children

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