Discontinuities in elastic constants are detected at the superconducting transition of layered organic conductors κ-(BEDT-TTF)2X by longitudinal and transverse ultrasonic velocity measurements. Symmetry arguments show that discontinuities in shear elastic constants can be explained in the orthorhombic compound only if the superconducting order parameter has a mixed character that can be of two types, either A1g + B1g or B2g + B3g in the classification of irreducible representations of the orthorhombic point group D 2h . Consistency with other measurements suggests that the A1g + B1g (dxy + d z(x+y) ) possibility is realized. Such clear symmetry-imposed signatures of mixed order parameters have not been observed in other superconducting compounds.PACS numbers: 74.70. Kn,74.25.Ld,74.20.Pp Unconventional, non s-wave, superconductors in solids seem ubiquitously associated with strong electronic correlations. This is the case in a wide variety of compounds that include heavy fermions, ruthenates, cuprates as well as quasi-two-dimensional half-filled organic charge transfer salts κ-(ET) 2 X (ET = BEDT-TTF) [1]. In most cases gaps with nodes are observed, but the exact symmetry of the unconventional superconducting order parameter is uncontroversial only in the cuprates.In this letter, we focus on the layered organics that exhibit antiferromagnetism and Mott insulating behavior, as the cuprates, and establish the two-component nature of the singlet order parameter in the orthorhombic compound κ-(ET) 2 Cu[N(CN) 2 ]Br. Previous studies suggest d-wave pairing with nodes, although s-wave symmetry is sometimes seen. Measurements sensitive to the k-space dispersion, such as scanning tunneling spectroscopy [2] and thermal conductivity [3], favor d xy symmetry, namely nodes along the nearest neighbor bonds (or equivalenty, between the orthorhombic axes). Moreover, theoretical calculations based either on spin-fluctuation mediated superconductivity [4,5,6,7] or on quantum cluster methods [8,9] and variational approaches [10] for the Hubbard model, support the anisotropic d-wave picture with a prevailing d x 2 −y 2 symmetry. Nevertheless, none of these calculations has considered interlayer hopping, which, as we will show, is necessary to explain the experimental data that we present.The ultrasonic probe is extremely sensitive to gap anisotropies as the attenuation and velocity depend on both the direction of wave propagation and the direction of polarization. Attenuation experiments on UPt 3 [11, 12] and of Sr 2 RuO 4 [13] perfectly illustrate how the unconventional gap structure can be unraveled by such a versatile technique. In organic charge transfer salts how- * Present address: Department of Physics and Astronomy, University of British Columbia, Vancouver, BC, Canada V6T 1Z4 ever, attenuation experiments are hampered by the small size and the shape of single crystals. Nevertheless, one experiment was successful for the κ-(ET) 2 Cu[N(CN) 2 ]Br compound [14], but the interpretation of the results was complicated by a phas...