Paired Ion Reversed-Phase HPLC Assay for the Simultaneous Determination of Iothalamic Acid and Para Aminohippuric Acid in Plasma

Jayewardene, Anura L.; Seneviratne, Ananda K.; Gambertoglio, John G.

doi:10.1080/10826079408013488

Cited by 5 publications

(2 citation statements)

References 11 publications

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“…Iothalamate concentrations were determined using a modified high performance liquid chromatography method adapted from that of Jayewardene et al 11 Separation was achieved with a C Se , 4-mm (250 ϫ 4.6 mm) column at 254 nm. The mobile phase was composed of 22% methanol, 3% acetonitrile, and 1.75 mM tetrabutylammonium chloride in 12 mM potassium phosphate (pH 7.5).…”

Section: Methodsmentioning

confidence: 99%

Effects of Renal Impairment on the Disposition of Orally Administered Enalapril, Benazepril, and Their Active Metabolites

Lefebvre

Laroute

Concordet

et al. 1999

Veterinary Internal Medicne

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The pharmacokinetics of benazepril, enalapril, and their active metabolites (benazeprilat and enalaprilat) were compared after a single administration of each product by the oral route at the recommended dosage (0.5 mg/kg for both drugs) in the dog before and after moderate experimental renal impairment. Ten dogs were randomly assigned to 2 groups of 5 animals in a 2-period crossover design for angiotensin-converting enzyme inhibitor administration. Renal failure was surgically induced by right nephrectomy and electrocoagulation of the remaining kidney. Renal mass reduction induced a significant decrease (P Ͻ .001) in glomerular filtration rate (GFR) (1.7 Ϯ 0.3 versus 3.3 Ϯ 0.7 mL/kg/minute). No significant differences before and after surgery were observed for enalapril and benazepril kinetics. The area under the curve (AUC) for enalaprilat increased after surgery from 23.6 Ϯ 14.7 to 42.4 Ϯ 20.9 g·minute/mL (P Ͻ .01). Mean peak plasma concentration (C max ) was increased in the impaired dogs (59.1 Ϯ 23.3 versus 43.9 Ϯ 32.9 ng/mL), but this variation was not significant (P Ͼ .05). Renal failure had no significant effect on AUC for benazeprilat (13.8 Ϯ 9.8 versus 14.9 Ϯ 5.0 g·minute/mL) (P Ͼ .05), but C max decreased significantly (from 55.0 Ϯ 26.4 to 31.9 Ϯ 17.7 ng/mL) (P Ͻ .05). Multiple regression analysis showed that both GFR and AUC for enalapril were highly significant variables that explained the variation in AUC for enalaprilat (R 2 ϭ .86, P Ͻ .001) but not for benazeprilat (R 2 ϭ .12, P Ͼ .05). The results of this study indicate that exposure to enalaprilat, but not to benazeprilat, is increased in dogs with subclinical renal impairment.

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Section: Methodsmentioning

confidence: 99%

Effects of Renal Impairment on the Disposition of Orally Administered Enalapril, Benazepril, and Their Active Metabolites

Lefebvre

Laroute

Concordet

et al. 1999

Veterinary Internal Medicne

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“…Plasma iothalamate, acetaminophen, and sulfapyridine concentrations were measured by described validated HPLC methods (24,34). Plasma xylose concentrations were determined by spectrophotometry (33).…”

Section: Assaysmentioning

confidence: 99%

Small bowel motility and colonic transit are altered in dogs with moderate renal failure

Lefebvre

Ferré

Watson

et al. 2001

American Journal of Physiology-Regulatory, Integrative and Comp

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Although gastrointestinal complications are common in patients with renal disease, the effects of renal dysfunction on bowel motility and gut transit times are not well known. We assessed gastrointestinal electromyographic activity, gastric emptying rate, orocolonic transit time, oroanal transit time, and xylose absorption before and after surgically inducing a 66% decrease in glomerular filtration rate in dogs. Moderate renal failure induced no gross or microscopic gastrointestinal lesions but caused a 16-42% increase in gastrointestinal motility indexes. We found a 24% decrease in the propagation velocity of the myoelectrical migrating complex in the duodenojejunal segment, a 30% decrease in phase I duration in duodenal and jejunal regions, a 20% increase in the total irregular electrical activity of the small intestine, and a 22% increase in duration of the meal response in the duodenum and jejunum. Renal failure did not change xylose absorption, gastric emptying rate, and orocolonic transit time but decreased colonic transit time by 38%. The mean weight of feces was increased. These results indicate that moderate renal failure alters duodenojejunal motility and decreases colonic transit time.

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Rapid determination of p-aminohippuric acid in serum and urine by high-performance liquid chromatography

Marsilio

Dall’Amico

Montini

et al. 1997

Journal of Chromatography B: Biomedical Sciences and Applicatio

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Paired Ion Reversed-Phase HPLC Assay for the Simultaneous Determination of Iothalamic Acid and Para Aminohippuric Acid in Plasma

Cited by 5 publications

References 11 publications

Effects of Renal Impairment on the Disposition of Orally Administered Enalapril, Benazepril, and Their Active Metabolites

Effects of Renal Impairment on the Disposition of Orally Administered Enalapril, Benazepril, and Their Active Metabolites

Small bowel motility and colonic transit are altered in dogs with moderate renal failure

Rapid determination of p-aminohippuric acid in serum and urine by high-performance liquid chromatography

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