PAIR2 is essential for homologous chromosome synapsis in rice meiosis I

Nonomura, Ken‐Ichi; Nakano, Michihiko; Eiguchi, Mitsugu; Suzuki, Tatsujiro; Kurata, Nori

doi:10.1242/jcs.02736

Cited by 133 publications

(152 citation statements)

References 49 publications

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“…RPA2c-containing RPA could bind to axis association sites at S/G2 phase determined by REC8. This is further supported by the overlapping localization of RPA2c and PAIR2, a rice ortholog of HOP1 (Nonomura et al, 2006). Because RPA is a ssDNA binding protein and loading of RPA2c on chromosomes depends on REC8, we further speculate that AT-rich axis association sites are easily accessed for the loading of REC8 on chromatin during premeiotic S-phase and then may produce ssDNA regions in situ, and this is the prerequisite for the consequent loading of RPA2c to this region.…”

Section: Role Of Rpa2c In Meiotic Recombinationsupporting

confidence: 51%

“…REC8 and PAIR2 are established markers of meiotic events in rice (Nonomura et al, 2006;Wang et al, 2009). At S/G2 phase, the discrete REC8 and RPA2c punctate foci appear simultaneously with nearly equal numbers, which almost all colocalize ( Figure 5A).…”

Section: Rpa2c Colocalizes With Rec8 and Pair2 At Premeiotic S/g2 Phasementioning

confidence: 99%

“…REC8 and HOP1 are conserved across diverse species, and their homologs (At-REC8/At-SYN1/At-DIF1 [three names for the same gene] and Os-REC8 for REC8; ASY1 and PAIR2 for HOP1) have also been identified in Arabidopsis and rice. Depletion of REC8 causes chromosome fragmentation and formation of univalents (Cai et al, 2003;Chelysheva et al, 2005;Shao et al, 2011), and mutation of ASY1 and PAIR2 also results in defects in synapsis and CO formation in Arabidopsis and rice, respectively (Caryl et al, 2000;Nonomura et al, 2006;SanchezMoran et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Replication Protein A2c Coupled with Replication Protein A1c Regulates Crossover Formation during Meiosis in Rice

Chang

Xin

et al. 2013

The Plant Cell

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Replication protein A (RPA) is a conserved heterotrimeric protein complex comprising RPA1, RPA2, and RPA3 subunits involved in multiple DNA metabolism pathways attributable to its single-stranded DNA binding property. Unlike other species possessing a single RPA2 gene, rice (Oryza sativa) possesses three RPA2 paralogs, but their functions remain unclear. In this study, we identified RPA2c, a rice gene preferentially expressed during meiosis. A T-DNA insertional mutant (rpa2c) exhibited reduced bivalent formation, leading to chromosome nondisjunction. In rpa2c, chiasma frequency is reduced by ;78% compared with the wild type and is accompanied by loss of the obligate chiasma. The residual ;22% chiasmata fit a Poisson distribution, suggesting loss of crossover control. RPA2c colocalized with the meiotic cohesion subunit REC8 and the axis-associated protein PAIR2. Localization of REC8 was necessary for loading of RPA2c to the chromosomes. In addition, RPA2c partially colocalized with MER3 during late leptotene, thus indicating that RPA2c is required for class I crossover formation at a late stage of homologous recombination. Furthermore, we identified RPA1c, an RPA1 subunit with nearly overlapping distribution to RPA2c, required for ;79% of chiasmata formation. Our results demonstrate that an RPA complex comprising RPA2c and RPA1c is required to promote meiotic crossovers in rice.

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Section: Role Of Rpa2c In Meiotic Recombinationsupporting

confidence: 51%

Section: Rpa2c Colocalizes With Rec8 and Pair2 At Premeiotic S/g2 Phasementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Replication Protein A2c Coupled with Replication Protein A1c Regulates Crossover Formation during Meiosis in Rice

Chang

Xin

et al. 2013

The Plant Cell

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“…PAIR3 is an axis-associated protein in rice, and it localizes to the chromosome core during prophase I and associates with both unsynapsed axial elements and synapsed lateral elements (Wang et al, 2011a). PAIR2 plays an essential role in promoting homologous chromosome synapsis, and its distribution can well reflect the distribution of axial elements in early stages as a protein that associates with axial elements in rice (Nonomura et al, 2006). OsMER3, a ZMM protein, has an important role in the formation of COs and may directly participate in double strand break repair in rice (Wang et al, 2009).…”

Section: Osrec8 Establishes a Platform For Homologous Recombination Amentioning

confidence: 99%

OsREC8 Is Essential for Chromatid Cohesion and Metaphase I Monopolar Orientation in Rice Meiosis

Shao

Tang²,

Wang³

et al. 2011

Plant Physiol.

119

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The successful transmission of chromosomes during mitosis and meiosis relies on the establishment and subsequent release of cohesion between replicated chromatids. Cohesion is mediated by a four-subunit structural maintenance of chromosome complex, called cohesins. REC8 is a key component of this meiotic cohesion complex in most model organisms studied to date. Here, we isolated and dissected the functions of OsREC8, a rice (Oryza sativa) REC8 homolog, using two null Osrec8 mutants. We showed that OsREC8 encodes a protein that localized to meiotic chromosomes from approximately meiotic interphase to metaphase I. Homologous pairing and telomere bouquet formation were abnormal in Osrec8 meiocytes. Furthermore, fluorescent in situ hybridization experiments on Osrec8 meiocytes demonstrated that the mutation eliminated meiotic centromeric cohesion completely during prophase I and also led to the bipolar orientation of the kinetochores during the first meiotic division and accordingly resulted in premature separation of sister chromatid during meiosis I. Immunolocalization analyses revealed that the loading of PAIR2, PAIR3, OsMER3, and ZEP1 all depended on OsREC8. By contrast, the presence of the OsREC8 signal in pair2, pair3, Osmer3, and zep1 mutants indicated that the loading of OsREC8 did not rely on these four proteins. These results suggest that OsREC8 has several essential roles in the meiotic processes.

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“…Antibodies against several other proteins involved in meiosis, including OsREC8, PAIR2, OsMER3 and ZEP1, were used to question whether their patterns were altered in Osam1-1. Among them, PAIR2 is the rice homolog of S. cerevisiae HOP1 and Arabidopsis ASY1, which associates with AEs at leptotene and zygotene and is removed from the AEs when homologous chromosomes have synapsed [39]. The distribution pattern of PAIR2 reflects the status of the chromosome axes.…”

Section: Meiotic Element Installation Is Disrupted In Osam1-1mentioning

confidence: 99%

OsAM1 is required for leptotene-zygotene transition in rice

et al. 2011

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The events occurring at the onset of meiosis have not been fully elucidated. In the present study, OsAM1 was identified in rice (Oryza sativa L.) by map-based cloning. OsAM1, a homolog of Arabidopsis SWI1 and maize AM1, encodes a protein with a coiled-coil domain in its central region. In the Osam1 mutant, pollen mother cells are arrested at leptotene, showing that OsAM1 is required for the leptotene-zygotene transition. Immunocytological analysis revealed that OsAM1 exists as foci in early prophase I meiocytes. Very faint OsREC8 foci persisted in the Osam1 mutant, indicating that OsAM1 is not required for the initial meiotic recruitment of OsREC8. In the absence of OsAM1, many other critical meiotic components, including PAIR2, ZEP1 and OsMER3, could not be correctly installed onto chromosomes. In contrast, in pair2, Osmer3 and zep1 mutants, OsAM1 could be loaded normally, suggesting that OsAM1 plays a fundamental role in building the proper chromosome structure at the beginning of meiosis.

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PAIR2 is essential for homologous chromosome synapsis in rice meiosis I

Abstract: SummaryPAIR2 is essential for homologous chromosome synapsis in rice meiosis I

Cited by 133 publications

References 49 publications

Replication Protein A2c Coupled with Replication Protein A1c Regulates Crossover Formation during Meiosis in Rice

Replication Protein A2c Coupled with Replication Protein A1c Regulates Crossover Formation during Meiosis in Rice

OsREC8 Is Essential for Chromatid Cohesion and Metaphase I Monopolar Orientation in Rice Meiosis

OsAM1 is required for leptotene-zygotene transition in rice

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