“…Therefore, for the complete characterization of cancer, we need to characterize the whole spectrum of cell clones propelling collectively its growth -one cell clone at a time. The very recent attempts in this regard involved studies using fluorescent activated cell sorting and/or enrichment (FACS), magnetic activated cell sorting and/or enrichment (MACS), laser microdissection, morphological and immunocytochemical analysis, of proteomes on populations of single cancer cells by mass spectroscopy, transcriptome analysis through RTPCR and sequencing, nuclei and chromosome isolation, comparative genomic hybridization (CGH), whole genome amplification, genomic and nested sequencing (Fiegler et al, 2007;Fuhrmann et al, 2008;Gribble et al, 2004;Guillaud-Bataille et al, 2004;Klein et al, 1999;Ley et al, 2008;Navin et al, 2010;Pan et al, 2008;Puente et al, 2011;Roberts et al, 2004;Spits et al, 2006). However, those protocols, published to this date, did not address all the problems.…”