a b s t r a c tAnti-self-dual (ASD) solutions to the Yang-Mills equation (or instantons) over an antiself-dual 4-manifold, which are invariant under an appropriate action of a threedimensional Lie group, give rise, via twistor construction, to isomonodromic deformations of connections on CP 1 having four simple singularities. As is well known, such deformations are governed by the sixth Painlevé equation Pvi (α, β, γ , δ). We work out the particular case of the SU 2 -action on S 4 , obtained from the irreducible representation on R 5 . In particular, we express the parameters (α, β, γ , δ) in terms of the instanton number. The present paper contains the proof of the result announced in [Richard Muñiz Manasliski, Painlevé VI equation from invariant instantons, in: Geometric and Topological Methods for