Background:
Phonotimpus pennimani
(Araneae, Phrurolithidae) is a
small-sized (3-5 mm) spider endemic to the Tacaná volcano in Chiapas,
Mexico, where it is found in soil litter of cloud forests and coffee
plantations. Its venom composition has so far not been investigated, partly
because it is not a species of medical significance. However, it does have
an important impact on the arthropod populations of its natural habitat.
Methods:
Specimens were collected in Southeastern Mexico (Chiapas) and identified
taxonomically by morphological characteristics. A partial sequence from the
mitochondrial gene
coxI
was amplified. Sequencing on the
Illumina platform of a transcriptome library constructed from 12 adult
specimens revealed 25 toxin or toxin-like genes. Transcripts were validated
(RT-qPCR) by assessing the differential expression of the toxin-like
PpenTox1 transcript and normalising with housekeeping genes.
Results:
Analysis of the
coxI
-gene revealed a similarity to other
species of the family Phrurolithidae. Transcriptome analysis also revealed
similarity with venom components of species from the families Ctenidae,
Lycosidae, and Sicariidae. Expression of the toxin-like PpenTox1 gene was
different for each developmental stage (juvenile or adult) and also for both
sexes (female or male). Additionally, a partial sequence was obtained for
the toxin-like PpenTox1 from DNA.
Conclusion:
Data from the amplification of the mitochondrial
coxI
gene
confirmed that
P. pennimani
belongs to the family
Phrurolithidae. New genes and transcripts coding for venom components were
identified.