PAHs reduce DNA synthesis and delay cell division in the widespread primary producer Prochlorococcus

Cerezo, María Isabel; Agustı́, Susana

doi:10.1016/j.envpol.2014.09.023

Cited by 19 publications

(12 citation statements)

References 53 publications

(79 reference statements)

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“…S4), indicating a harmful effect of ADOC compounds to the community. For instance, PAHs have been identified as inducing phytotoxicity to Prochlorococcus by reducing DNA synthesis and delaying cell division (Cerezo and Agustí, 2015). For instance, PAHs have been identified as inducing phytotoxicity to Prochlorococcus by reducing DNA synthesis and delaying cell division (Cerezo and Agustí, 2015).…”

Section: Changes In Bacterioplankton Community Compositionmentioning

confidence: 99%

“…PAHs, particularly benzo[a]pyrene, and other hydrophobic chemicals, such as chlorinated aliphatic compounds, can inhibit cell growth through the formation of DNA-PAH adducts (Kabelitz et al, 2003;Ewa and Danuta, 2017). For instance, PAHs have been identified as inducing phytotoxicity to Prochlorococcus by reducing DNA synthesis and delaying cell division (Cerezo and Agustí, 2015). Other studies have found that PAHs cause DNA damage in E. coli (Kim et al, 2007).…”

Section: Changes In Bacterioplankton Community Compositionmentioning

confidence: 99%

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Microbial responses to anthropogenic dissolved organic carbon in the Arctic and Antarctic coastal seawaters

Cerro‐Gálvez

Casal

Lundin

et al. 2019

Environmental Microbiology

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Summary Thousands of semi‐volatile hydrophobic organic pollutants (OPs) reach open oceans through atmospheric deposition, causing a chronic and ubiquitous pollution by anthropogenic dissolved organic carbon (ADOC). Hydrophobic ADOC accumulates in cellular lipids, inducing harmful effects on marine biota, and can be partially prone to microbial degradation. Unfortunately, their possible effects on microorganisms, key drivers of global biogeochemical cycles, remain unknown. We challenged coastal microbial communities from Ny‐Ålesund (Arctic) and Livingston Island (Antarctica) with ADOC concentrations within the range of oceanic concentrations in 24 h. ADOC addition elicited clear transcriptional responses in multiple microbial heterotrophic metabolisms in ubiquitous groups such as Flavobacteriia, Gammaproteobacteria and SAR11. Importantly, a suite of cellular adaptations and detoxifying mechanisms, including remodelling of membrane lipids and transporters, was detected. ADOC exposure also changed the composition of microbial communities, through stimulation of rare biosphere taxa. Many of these taxa belong to recognized OPs degraders. This work shows that ADOC at environmentally relevant concentrations substantially influences marine microbial communities. Given that emissions of organic pollutants are growing during the Anthropocene, the results shown here suggest an increasing influence of ADOC on the structure of microbial communities and the biogeochemical cycles regulated by marine microbes.

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Section: Changes In Bacterioplankton Community Compositionmentioning

confidence: 99%

Section: Changes In Bacterioplankton Community Compositionmentioning

confidence: 99%

Microbial responses to anthropogenic dissolved organic carbon in the Arctic and Antarctic coastal seawaters

Cerro‐Gálvez

Casal

Lundin

et al. 2019

Environmental Microbiology

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“…They cover more than 70% of the earth's surface, hold 97% of the world's water, host some of the planet's most diverse ecosystems, and support economies in countries around the world [1,2]. Microscopic organisms in the seas are a major source of atmospheric oxygen [3,4,5,6]. By absorbing more than 90% of the excess heat released into the earth's environment and nearly one-third of carbon dioxide emissions, the oceans slow planetary warming and stabilize the global climate [7].…”

Section: Introductionmentioning

confidence: 99%

Human Health and Ocean Pollution

Landrigan

Stegeman

Fleming

et al. 2020

Annals of Global Health

353

170

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Methods: Topic-focused reviews that examine the effects of ocean pollution on human health, identify gaps in knowledge, project future trends, and offer evidence-based guidance for effective intervention. Environmental Findings: Pollution of the oceans is widespread, worsening, and in most countries poorly controlled. It is a complex mixture of toxic metals, plastics, manufactured chemicals, petroleum, urban and industrial wastes, pesticides, fertilizers, pharmaceutical chemicals, agricultural runoff, and sewage. More than 80% arises from land-based sources. It reaches the oceans through rivers, runoff, atmospheric deposition and direct discharges. It is often heaviest near the coasts and most highly concentrated along the coasts of low-and middle-income countries. Plastic is a rapidly increasing and highly visible component of ocean pollution, and an estimated 10 million metric tons of plastic waste enter the seas each year. Mercury is the metal pollutant of greatest concern in the oceans; it is released from two main sources-coal combustion and small-scale gold mining. Global spread of industrialized agriculture with increasing use of chemical fertilizer leads to extension of Harmful Algal Blooms (HABs) to previously unaffected regions. Chemical pollutants are ubiquitous and contaminate seas and marine organisms from the high Arctic to the abyssal depths. Ecosystem Findings: Ocean pollution has multiple negative impacts on marine ecosystems, and these impacts are exacerbated by global climate change. Petroleum-based pollutants reduce photosynthesis in marine microorganisms that generate oxygen. Increasing absorption of carbon dioxide into the seas causes ocean acidification, which destroys coral reefs, impairs shellfish development, dissolves calcium-containing microorganisms at the base of the marine food web, and increases the toxicity of some pollutants. Plastic pollution threatens marine mammals, fish, and seabirds and accumulates in large mid-ocean gyres. It breaks down into microplastic and nanoplastic particles containing multiple manufactured chemicals that can enter the tissues of marine organisms, including species consumed by humans. Industrial releases, runoff, and sewage increase frequency and severity of HABs, bacterial pollution, and anti-microbial resistance. Pollution and sea surface warming are triggering poleward migration of dangerous pathogens such as the Vibrio species. Industrial discharges, pharmaceutical wastes, pesticides, and sewage contribute to global declines in fish stocks. Human Health Findings: Methylmercury and PCBs are the ocean pollutants whose human health effects are best understood. Exposures of infants in utero to these pollutants through maternal consumption of contaminated seafood can damage developing brains, reduce IQ and increase children's risks for autism, ADHD and learning disorders. Adult exposures to methylmercury increase risks for cardiovascular disease and dementia. Manufactured chemicals-phthalates, bisphenol A, flame retardants, and perfluorinated chemicals...

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“…Phytoplankton are particularly vulnerable to pollutants, and exposure to toxic PAHs is among the most severe threats to their abundance and physiology, especially in coastal habitats. Adverse effects of PAHs in marine phytoplankton include photosynthetic inhibition (Othman et al, 2012), DNA damage (Cerezo & Agustí, 2015), reduced gene expression (Bopp & Lettieri, 2007), oxidative stress (Vega-López et al, 2013), influence on abundance and distribution (Echeveste et al, 2016;Kottuparambil & Agusti, 2018), and trophic transfer to consumers (Froehner et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Cell-by-cell estimation of PAH sorption and subsequent toxicity in marine phytoplankton

Kottuparambil

Agustı́

2020

Chemosphere

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Polycyclic Aromatic Hydrocarbons (PAHs) have elicited increasing concern due to their ubiquitous occurrence in coastal marine environments and resultant toxicity in organisms. Due to their lipophilic nature, PAHs tend to accumulate in phytoplankton cells and thus subsequently transfer to other compartments of the marine ecosystem. The intrinsic fluorescence properties of PAHs in the ultraviolet (UV)/blue spectral range have recently been exploited to investigate their uptake modes, localization, and aggregation in various biological tissues. Here, we quantitatively evaluate the sorption of two model PAHs (phenanthrene and pyrene) in three marine phytoplankton species (Chaetoceros tenuissimus, Thalassiosira sp. and Proteomonas sp.) using a combined approach of UV excitation flow cytometry and fluorescence microscopy. Over a 48-h exposure to a gradient of PAHs, Thalassiosira sp. showed the highest proportion of PAH-sorbed cells (29% and 97% of total abundance for phenanthrene and pyrene, respectively), which may be attributed to its relatively high total lipid content (33.87 percent dry weight). Moreover, cell-specific pulse amplitude modulation (PAM) microscope fluorometry revealed that PAH sorption significantly reduced the photosynthetic quantum efficiency (Fv/Fm) of individual phytoplankton cells. We describe a rapid and precise hybrid method for the detection of sorption of PAHs on phytoplankton cells. Our results emphasize the ecologically relevant sub-lethal effects of PAHs in phytoplankton at the cellular level, even at concentrations where no growth inhibition was apparent. This work is the first study to address the cellspecific impacts of fluorescent toxicants in a more relevant toxicant-sorbed subpopulation; these cell-specific impacts have to date been unidentified in traditional population-based phytoplankton toxicity assays.

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PAHs reduce DNA synthesis and delay cell division in the widespread primary producer Prochlorococcus

Cited by 19 publications

References 53 publications

Microbial responses to anthropogenic dissolved organic carbon in the Arctic and Antarctic coastal seawaters

Microbial responses to anthropogenic dissolved organic carbon in the Arctic and Antarctic coastal seawaters

Human Health and Ocean Pollution

Cell-by-cell estimation of PAH sorption and subsequent toxicity in marine phytoplankton

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