PagERF81 regulates lignin biosynthesis and xylem cell differentiation in poplar

Zhao, Xinwei; Wang, Qiao; Wang, Dian; Guo, Wei; Hu, Meng-Xuan; Liu, Ying-Li; Zhou, Gongke; Chai, Guohua; Zhao, Shutang; Lu, Mengzhu

doi:10.1111/jipb.13453

Cited by 11 publications

(3 citation statements)

References 54 publications

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“…Lignin biosynthesis has been studied intensively in recent decades and many studies reported that the TF-regulated lignin biosynthesis pathways were correlated with stress resistance in plants [ 35 ]. Previous studies showed that AP2/ERF family members could regulate the lignin biosynthesis genes in response to stress [ 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 ]. In the current study, RNA-Seq data indicated that the phenylpropanoid biosynthesis pathway was enriched in overexpression- ScDREB10 plants under osmotic stress, which is in accordance with the high-lignin-biosynthesis-related gene expression level in OE plants under osmotic stress ( Figure 4 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Profiles Reveals ScDREB10 from Syntrichia caninervis Regulated Phenylpropanoid Biosynthesis and Starch/Sucrose Metabolism to Enhance Plant Stress Tolerance

Liang,

Li,

Lei

et al. 2024

Plants

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Desiccation is a kind of extreme form of drought stress and desiccation tolerance (DT) is an ancient trait of plants that allows them to survive tissue water potentials reaching −100 MPa or lower. ScDREB10 is a DREB A-5 transcription factor gene from a DT moss named Syntrichia caninervis, which has strong comprehensive tolerance to osmotic and salt stresses. This study delves further into the molecular mechanism of ScDREB10 stress tolerance based on the transcriptome data of the overexpression of ScDREB10 in Arabidopsis under control, osmotic and salt treatments. The transcriptional analysis of weight gene co-expression network analysis (WGCNA) showed that “phenylpropanoid biosynthesis” and “starch and sucrose metabolism” were key pathways in the network of cyan and yellow modules. Meanwhile, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of differentially expressed genes (DEGs) also showed that “phenylpropanoid biosynthesis” and “starch and sucrose metabolism” pathways demonstrate the highest enrichment in response to osmotic and salt stress, respectively. Quantitative real-time PCR (qRT-PCR) results confirmed that most genes related to phenylpropanoid biosynthesis” and “starch and sucrose metabolism” pathways in overexpressing ScDREB10 Arabidopsis were up-regulated in response to osmotic and salt stresses, respectively. In line with the results, the corresponding lignin, sucrose, and trehalose contents and sucrose phosphate synthase activities were also increased in overexpressing ScDREB10 Arabidopsis under osmotic and salt stress treatments. Additionally, cis-acting promoter element analyses and yeast one-hybrid experiments showed that ScDREB10 was not only able to bind with classical cis-elements, such as DRE and TATCCC (MYBST1), but also bind with unknown element CGTCCA. All of these findings suggest that ScDREB10 may regulate plant stress tolerance by effecting phenylpropanoid biosynthesis, and starch and sucrose metabolism pathways. This research provides insights into the molecular mechanisms underpinning ScDREB10-mediated stress tolerance and contributes to deeply understanding the A-5 DREB regulatory mechanism.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Profiles Reveals ScDREB10 from Syntrichia caninervis Regulated Phenylpropanoid Biosynthesis and Starch/Sucrose Metabolism to Enhance Plant Stress Tolerance

Liang,

Li,

Lei

et al. 2024

Plants

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“…pyramidalis [9], etc. On this basis, the mechanisms of functional genes involved in various biological processes of poplar have also been studied, such as growth and development [10,11], stress response [12,13], wood formation [14][15][16], and sexual differentiation. However, as a woody plant, poplar has some unique features in terms of life cycle, morphological structure, and environmental adaptability [6,12].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Expression Profiling of Velvet Complex Transcription Factors in Populus alba × Populus glandulosa

Hao,

Yan,

2024

IJMS

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The discovery of new genes with novel functions is a major driver of adaptive evolutionary innovation in plants. Especially in woody plants, due to genome expansion, new genes evolve to regulate the processes of growth and development. In this study, we characterized the unique VeA transcription factor family in Populus alba × Populus glandulosa, which is associated with secondary metabolism. Twenty VeA genes were characterized systematically on their phylogeny, genomic distribution, gene structure and conserved motif, promoter binding site, and expression profiling. Furthermore, through ChIP-qPCR, Y1H, and effector-reporter assays, it was demonstrated that PagMYB128 directly regulated PagVeA3 to influence the biosynthesis of secondary metabolites. These results provide a basis for further elucidating the function of VeAs gene in poplar and its genetic regulation mechanism.

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“…Despite the well‐established correlation between vessel traits and hydraulic conductance, the precise mechanisms by which trees coordinate vessel development in response to drought remain incompletely understood. Notably, transgenic poplars overexpressing PtrERF139 , PtaERF194 , and PagERF81 have shown altered vessel characteristics, including reduced vessel size, increased vessel frequency, and enhanced lignin deposition (Wessels et al ., 2019; Wang et al ., 2022; X. W. Zhao et al ., 2023). These findings suggest the potential involvement of ERF transcription factors in regulating adaptive vessel development in response to drought in Populus .…”

Section: Introductionmentioning

confidence: 99%

The AP2/ERF transcription factor PtoERF15 confers drought tolerance via JA‐mediated signaling in Populus

Kong,

Song,

Wei

et al. 2023

New Phytologist

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Summary Drought stress is one of the major limiting factors for the growth and development of perennial trees. Xylem vessels act as the center of water conduction in woody species, but the underlying mechanism of its development and morphogenesis under water‐deficient conditions remains elucidation. Here, we identified and characterized an osmotic stress‐induced ETHYLENE RESPONSE FACTOR 15 (PtoERF15) and its target, PtoMYC2b, which was involved in mediating vessel size, density, and cell wall thickness in response to drought in Populus tomentosa. PtoERF15 is preferentially expressed in differentiating xylem of poplar stems. Overexpression of PtoERF15 contributed to stem water potential maintaining, thus promoting drought tolerance. RNA‐Seq and biochemical analysis further revealed that PtoERF15 directly regulated PtoMYC2b, encoding a switch of JA signaling pathway. Additionally, our findings verify that three sets of homologous genes from NAC (NAM, ATAF1/2, and CUC2) gene family: PtoSND1‐A1/A2, PtoVND7‐1/7‐2, and PtoNAC118/120, as the targets of PtoMYC2b, are involved in the regulation of vessel morphology in poplar. Collectively, our study provides molecular evidence for the involvement of the PtoERF15‐PtoMYC2b transcription cascade in maintaining stem water potential through the regulation of xylem vessel development, ultimately improving drought tolerance in poplar.

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PagERF81 regulates lignin biosynthesis and xylem cell differentiation in poplar

Cited by 11 publications

References 54 publications

Transcriptome Profiles Reveals ScDREB10 from Syntrichia caninervis Regulated Phenylpropanoid Biosynthesis and Starch/Sucrose Metabolism to Enhance Plant Stress Tolerance

Transcriptome Profiles Reveals ScDREB10 from Syntrichia caninervis Regulated Phenylpropanoid Biosynthesis and Starch/Sucrose Metabolism to Enhance Plant Stress Tolerance

Genome-Wide Identification and Expression Profiling of Velvet Complex Transcription Factors in Populus alba × Populus glandulosa

The AP2/ERF transcription factor PtoERF15 confers drought tolerance via JA‐mediated signaling in Populus

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