PAF-acetylhydrolase activity on Lp(a) before and during Cu2+-induced oxidative modification in vitro

Karabina, Sonia-Athena P.; Elisaf, Moses; Goudevenos, John; Siamopoulos, Kostas C.; Sideris, Dimitris A.; Tselepis, Alexandros D.

doi:10.1016/0021-9150(96)05872-8

Cited by 49 publications

(51 citation statements)

References 42 publications

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“…However, as the Lp(a) levels increase, there is proportionately less Lp-PLA 2 on apoB and more on Lp(a). This is consistent with the fact that on a molar basis, Lp(a) contains more Lp-PLA 2 mass (1.5-2 times) and activity (7 times) than LDL ( 13,14 ). However, as LDL is in excess of Lp(a) in most patients, there is globally more Lp-PLA 2 on LDL than Lp(a).…”

Section: Discussionsupporting

confidence: 85%

“…When all three Lp(a) groups were combined, there was a reduction in sPLA 2 mass (4.1 ± 7.7 versus 3.1 ± 8.4, P < 0.001) but not sPLA 2 activity (57.6 ± 30.1 versus 62.7 ± 33.7, P = 0.12). range of both small dense LDL, as previously shown for Lp-PLA 2 activity and Lp(a) ( 12,13,23 ). There appeared to be a similar amount of Lp-PLA 2 mass preapheresis in the low and intermediate Lp(a) groups, whereas the levels were lower in the high Lp(a) groups.…”

Section: Determination Of the Presence Of Oxpl And Lp-pla 2 Mass Diresupporting

confidence: 78%

“…In primary hypercholesterolemia, the LDL-associated Lp-PLA 2 is increased in parallel with the severity of hypercholesterolemia, the highest levels being present in homozygous familial hypercholesterolemia (FH) ( 11 ). Interestingly, in individuals with elevated Lp(a) levels, Lp-PLA 2 activity is higher on Lp(a) than on equimolar amounts of LDL ( 13,14 ). Lp-PLA 2 is present in vulnerable plaques, and increased Lp-PLA 2 levels are associated with increased CVD risk in both primary and secondary populations ( 15 ).…”

Section: Isolation Of Lipoprotein Density Fractionsmentioning

confidence: 99%

See 2 more Smart Citations

Acute impact of apheresis on oxidized phospholipids in patients with familial hypercholesterolemia

Arai

Orsoni

Mallat

et al. 2012

Journal of Lipid Research

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Section: Discussionsupporting

confidence: 85%

Section: Determination Of the Presence Of Oxpl And Lp-pla 2 Mass Diresupporting

confidence: 78%

Section: Isolation Of Lipoprotein Density Fractionsmentioning

confidence: 99%

See 1 more Smart Citation

Acute impact of apheresis on oxidized phospholipids in patients with familial hypercholesterolemia

Arai

Orsoni

Mallat

et al. 2012

Journal of Lipid Research

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“…The measurement of serum lipid parameters was performed by standard commercially available techniques as previously described (12). Lp(a) was measured using a monoclonal anti-Lp(a) antibody technique by the enzyme immunoassay Macra Lp(a) (Terumo Medical Corporation Diagnostic Division, Elkton, MD, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Positive samples were regarded as those which provided agglutination to a dilution of at least 1/400 and 1/640 respectively. Apo(a) isoform size was then determined by agarose gel electrophoresis followed by immunoblotting as described previously (12). A standard solution (Immuno-France, S.A.R.L., Rungis, France) containing six different isoforms corresponding to the F, B, S 1 , S 2 , S 3 , S 4 isoforms in the nomenclature of Utermann et al (11) was used in order to characterize our samples.…”

Section: Methodsmentioning

confidence: 99%

Lipoprotein (a) levels, apolipoprotein (a) phenotypes and thyroid autoimmunity

Bairaktari

Tselepis²,

Millionis³

et al. 1999

European Journal of Endocrinology

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It has been reported that euthyroid normolipidemic males and postmenopausal females exhibit significantly higher serum lipoprotein (a) (Lp(a)) levels compared with age-and sex-matched normolipidemic controls. However, it is well known that there is an inverse correlation between Lp(a) concentration and apolipoprotein (a) (apo(a)) isoform size. Thus, it is imperative to exclude differences in apo(a) isoform frequencies between subjects with or without thyroid autoimmunity in order to verify if there is an association between thyroid autoimmunity and increased Lp(a) concentration. To exclude such an effect of different apo(a) isoform frequencies, we determined apo(a) phenotypes in 22 patients (9 males and 13 postmenopausal females) with thyroid autoimmunity and in 64 (29 males and 35 females) age-and sex-matched individuals without thyroid autoimmunity (control group). There were no significant differences in the values of lipid parameters between the two groups, including Lp(a). We did not detect any significant differences in the apo(a) phenotype frequencies between the two groups. Additionally, in neither of the subgroups formed according to the presence of low molecular vs high molecular weight apo(a) isoforms were there any significant differences in median serum Lp(a) levels between patients with and without thyroid autoimmunity. Thus, our results contradict the previously reported association between thyroid autoimmunity and Lp(a) concentrations.

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Oxidized phospholipids and lipoprotein‐associated phospholipase A₂ (Lp‐PLA₂) in atherosclerotic cardiovascular disease: An update

2022

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Inflammation and oxidative stress conditions lead to a variety of oxidative modifications of lipoprotein phospholipids implicated in the occurrence and development of atherosclerotic lesions. Lipoprotein-associated phospholipase A2 (Lp-PLA 2 ) is established as an independent risk biomarker of atherosclerosis-related cardiovascular disease (ASCVD) and mediates vascular inflammation through the regulation of lipid metabolism in the blood and in atherosclerotic lesions. Lp-PLA 2 is associated with low-and high-density lipoproteins and Lipoprotein (a) in human plasma and specifically hydrolyzes oxidized phospholipids involved in oxidative stress modification. Several oxidized phospholipids (OxPLs) subspecies can be detoxified through enzymatic degradation by Lp-PLA 2 activation, forming lysophospholipids and oxidized nonesterified fatty acids (OxNEFAs). Lysophospholipids promote the expression of adhesion molecules, stimulate cytokines production (TNF-α, IL-6), and attract macrophages to the arterial intima. The present review article discusses new data on the functional roles of OxPLs and Lp-PLA 2 associated with lipoproteins.

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PAF-acetylhydrolase activity on Lp(a) before and during Cu2+-induced oxidative modification in vitro

Cited by 49 publications

References 42 publications

Acute impact of apheresis on oxidized phospholipids in patients with familial hypercholesterolemia

Acute impact of apheresis on oxidized phospholipids in patients with familial hypercholesterolemia

Lipoprotein (a) levels, apolipoprotein (a) phenotypes and thyroid autoimmunity

Oxidized phospholipids and lipoprotein‐associated phospholipase A₂ (Lp‐PLA₂) in atherosclerotic cardiovascular disease: An update

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PAF-acetylhydrolase activity on Lp(a) before and during Cu2+-induced oxidative modification in vitro

Cited by 49 publications

References 42 publications

Acute impact of apheresis on oxidized phospholipids in patients with familial hypercholesterolemia

Acute impact of apheresis on oxidized phospholipids in patients with familial hypercholesterolemia

Lipoprotein (a) levels, apolipoprotein (a) phenotypes and thyroid autoimmunity

Oxidized phospholipids and lipoprotein‐associated phospholipase A2 (Lp‐PLA2) in atherosclerotic cardiovascular disease: An update

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Product

Resources

About

Oxidized phospholipids and lipoprotein‐associated phospholipase A₂ (Lp‐PLA₂) in atherosclerotic cardiovascular disease: An update