Paeonol inhibits inflammatory response and protects chondrocytes by upregulating sirtuin 1

Abstract

Paeonol is the bioactive component in Paeonia lactiflora Pall., Cynanchum paniculatum and Paeonia × suffruticosa Andr. Paeonol has been previously demonstrated to inhibit the release of tumor necrosis factor α (TNF-α) and interluekin 6 (IL-6) in chondrocytes. Sirtuin 1 (SIRT1) is downregulated in degraded cartilage and paeonol could induce nuclear accumulation of SIRT1. Therefore, the present study aims to investigate the possible role of paeonol in chondrocyte inflammation and cartilage protection in osteoarthritis (OA) as well as its regulation of SIRT1. Primary chondrocytes from rat knee joints were transfected with short hairpin (sh) – SIRT1 and (or) paeonol prior to IL-1β exposure, and then inflammatory response, apoptosis, and extracellular matrix (ECM) degradation in the cells were evaluated concurrent with the activation of the nuclear factor κβ (NF-κβ) signaling pathway. Increased levels of TNF-α, IL-17, IL-6, matrix metalloproteinase 1 (MMP-1), MMP-3, and MMP-13 along with decreased tissue inhibitor of metalloproteinases 1 and type II collagen levels were found in IL-1β-stimulated chondrocytes. Chondrocyte apoptosis was elevated and the NF-κβ signaling pathway was activated in response to IL-1β treatment. Paeonol enhanced SIRT1 expression to inactivate the NF-κβ signaling pathway, thereby ameliorating inflammatory cytokine secretion, ECM degradation, and chondrocyte apoptosis. In conclusion, the results of the present study confirm the potential of paeonol as a candidate OA drug.

Huangqi Guizhi Wuwu decoction promotes M2 microglia polarization and synaptic plasticity via Sirt1/NF-κB/NLRP3 pathway in MCAO rats

“…Isoflavonoid formononetin was reported to attenuate renal tubular injury by upregulating Sirt1/PGC-1α pathway in diabetic nephropathy [ 44 ]. Paeonol form Radix paeoniae alba was also reported to inhibit inflammatory response in chondrocytes by upregulating Sirt1 [ 45 ]. Besides, bioactive chemicals in HGWD including calycosin can modulate NLRP3 and inhibit pyroptotic cell death [ 46 ].…”

Sirtuins in intervertebral disc degeneration: current understanding

“…Notably, safranal has the same effect (Liu et al 2022). Melatonin (Zhao et al 2022) and paeonol (Shang et al 2022) enhanced SIRT1 expression to inactivate the NF-κB signaling pathway, which ameliorated inflammatory cytokine secretion and extracellular matrix degradation.…”

Natural-derived acetophenones: chemistry and pharmacological activities

“…The concentration of 41, also, peaked in the dead tissue [53]. Four novel prenylated acetophenone compounds were isolated from Bosistoa selwynii identified as selwynone (121), pyranoselwynone (122), furanoselwynone (123), and isofuranoselwynone (124) [54]. Quader et alextracted and characterized acetophenones 41 from Acradenia frankliniae [55].…”