Paeonol induces apoptosis in human ovarian cancer cells

Yin, Juan Juan; Wu, Nanshun; Zeng, Fang; Cheng, Chao; Kang, Kai; Yang, Huan

doi:10.1016/j.acthis.2013.04.004

Cited by 26 publications

(27 citation statements)

References 28 publications

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“…Housing and all of the animal experiments were conducted according to the protocol approved by the Animal Care Committee of Nanjing University of Chinese Medicine. A549 cells (1-1.5×10 6 ) were harvested, washed, and resuspended in 200 μL serum-free medium, and were subcutaneously implanted into the right flanks of nude mice. Tumor volumes (mm 3 ) were defined using the following formula: V = (length × width 2 )/2.…”

Section: In Vivo Evaluation Of Pae-nps In a Xenograft Modelmentioning

confidence: 99%

“…[5][6][7][8][9][10][11][12][13] Pae has been reported to induce apoptosis in various cancer cells, including gastric cancer, esophageal cancer, ovarian cancer, and hepatocellular carcinoma. 5,6,8,12 One study reported that Pae had a radiosensitizing effect on lung adenocarcinoma cell both in vitro and in vivo. 9 This radiosensitizing effect is closely related to apoptotic induction, which is caused by the downregulation of the PI3K/Akt signaling pathway.…”

Section: Introductionmentioning

confidence: 99%

“…5,6,8,12 To examine the apoptotic effect of E-NPs, free Pae, and Pae-NPs, cells were treated with a dose of 50 μg/mL of these agents for 48 hours. Apoptosis was detected using flow cytometry assays with an annexin-V-FITC/PI kit.…”

mentioning

confidence: 99%

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Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects

Chen¹,

Feng²,

Hou³

et al. 2017

IJN

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Paeonol (Pae; 2′-hydroxy-4′-methoxyacetophenone) has attracted intense attention as a potential therapeutic agent against various cancers. However, the use of Pae is limited owing to its hydrophobicity. Recently, biodegradable polymeric nanoparticles with amphiphilic copolymers have been used as drug carriers; these have better bioavailability and are promising tumor-targeted drug delivery systems. In the current study, we prepared Pae-loaded nanoparticles (Pae-NPs) with amphiphilic block copolymers using nanoprecipitation. The physiochemical characteristics and antitumor effects of nanoparticles were evaluated in different cancer cells. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays showed substantial inhibition of cell growth by Pae-NPs. Moreover, lower doses of Pae-NPs inhibited cell growth more efficiently than the equivalent doses of free Pae. Inhibition was characterized by significant elevation of intracellular reactive oxygen species and subsequent inhibition of Akt and regulation of apoptotic proteins, which could be partly reversed by pretreatment with the antioxidant N-acetylcysteine. In vivo results also demonstrated that Pae-NPs could exert much stronger antitumor effects than free Pae. Therefore, Pae-NPs represent a promising delivery system to overcome the low solubility of Pae and enable its use in treating cancer.

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Section: In Vivo Evaluation Of Pae-nps In a Xenograft Modelmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects

Chen¹,

Feng²,

Hou³

et al. 2017

IJN

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“…Cells were cultured in 90% medium mixed with 10% serum at 37˝C in humidified atmosphere with 5% CO 2 and grown in T-75 flask with a feeding cycle of two to three days. The composition and procedures of preparing culture media for different cell lines were followed by instruction manual of suppliers [5,33].…”

Section: Cell Culturementioning

confidence: 99%

“…Moutan Cortex, the outer layer of the root of Moutan, is classified in the genus Paeonia and has been used for more than 1000 years. Paeonol is categorized as a flavonoid derivative and exhibits many remarkable biological effects, and it has been applied for anti-inflammatory [1,2], analgesic [2], antioxidant [3], antidiabetic [4], anticancer [5], and antiatherogenic purposes [6]. Paeonol was also found to protect against memory loss after ischemic stroke by reducing amyloid precursor protein (APP), beta-site APP cleaving enzyme (BACE), and apoptosis [7].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Evaluation of Aminothiazole-Paeonol Derivatives as Potential Anticancer Agents

et al. 2016

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Abstract:In this study, novel aminothiazole-paeonol derivatives were synthesized and characterized using 1 H-NMR, 13 C-NMR, IR, mass spectroscopy, and high performance liquid chromatography. All the new synthesized compounds were evaluated according to their anticancer effect on seven cancer cell lines. The experimental results indicated that these compounds possess high anticancer potential regarding human gastric adenocarcinoma (AGS cells) and human colorectal adenocarcinoma (HT-29 cells). Among these compounds, N-[4-(2-hydroxy-4-methoxyphenyl)thiazol-2-yl]-4-methoxybenzenesulfonamide (13c) had the most potent inhibitory activity, with IC 50 values of 4.0 µM to AGS, 4.4 µM to HT-29 cells and 5.8 µM to HeLa cells. The 4-fluoro-N-[4-(2-hydroxy-4-methoxyphenyl)thiazol-2-yl]benzenesulfonamide (13d) was the second potent compound, showing IC 50 values of 7.2, 11.2 and 13.8 µM to AGS , HT-29 and HeLa cells, respectively. These compounds are superior to 5-fluorouracil (5-FU) for relatively higher potency against AGS and HT-29 human cancer cell lines along with lower cytotoxicity to fibroblasts. Novel aminothiazole-paeonol derivatives in this work might be a series of promising lead compounds to develop anticancer agents for treating gastrointestinal adenocarcinoma.

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Paeonol inhibits B16F10 melanoma metastasis In vitro and In Vivo via disrupting proinflammatory cytokines‐mediated NF‐κB and STAT3 pathways

Zhang

Shi

et al. 2015

IUBMB Life

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Cancer related inflammation (CRI) is now recognized as the seventh hallmark in the pathogenesis of many types of malignancies. Paeonol, a natural phenolic component isolated from the root bark of Paeonia moutan, has significant antiinflammatory activity. Recently, accumulating body of research has revealed potent anti-tumor effects mediated by paeonol. However, little is known about its anticancer mechanism on the basis of CRI. In this study, we observed that paeonol exerted direct anticancer activity through inhibition of cell proliferation, induction of apoptosis, and evident antiinflammatory effects by reducing proinflammatory cytokines secretion (TNF-a, IL-1b, IL-6, and TGF-b) in the conditioned medium of B16F10 mouse melanoma cells. Interestingly, we found that paeonol significantly reversed motility phenotypes in TNF-a-or IL-6-induced B16F10 singe cell and collective migration and invasion in vitro, which were related to affecting epithelial-to-mesenchymal transition (EMT) makers and MMPs expression. In particular, paeonol disrupted both TNFa-activated NF-jB and IL-6-activated STAT3 signaling pathways in B16F10 cells. EMSA and luciferase assays showed that paeonol abrogated NF-jB binding and NF-jB-driven promoter activity in the presence of TNF-a. Finally, we showed that paeonol attenuated B16F10 spontaneous lung metastases in C57/BL6J mice with down-regulated levels of serum proinflammatory cytokines. Therefore, paeonol possessed antitumor activity in melanoma cells and mice model by 778IUBMB Life interruption of the aggressive feedback through proinflammatory cytokines mediated NF-jB and STAT3 signaling activation. These findings provide a novel treatment strategy that paeonol might be a promising versatile adjuvant therapy for cancer related inflammation. V C 2015 IUBMB Life, 67(10): [778][779][780][781][782][783][784][785][786][787][788] 2015

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Paeonol induces apoptosis in human ovarian cancer cells

Cited by 26 publications

References 28 publications

Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects

Delivery of paeonol by nanoparticles enhances its in vitro and in vivo antitumor effects

Synthesis and Evaluation of Aminothiazole-Paeonol Derivatives as Potential Anticancer Agents

Paeonol inhibits B16F10 melanoma metastasis In vitro and In Vivo via disrupting proinflammatory cytokines‐mediated NF‐κB and STAT3 pathways

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