Paeonol exerts potential activities to inhibit the growth, migration and invasion of human gastric cancer BGC823 cells via downregulating MMP-2 and MMP-9

Abstract

Paeonol (Pae) is an herbal extract that has attracted extensive attention for its anti-cancer effects demonstrated by a number of studies, which have predominantly demonstrated inhibition of cell proliferation and induction of apoptosis. The influence of Pae on cancer cell metastasis has been less widely reported. The present study aimed to investigate the under-reported effects of Pae on the growth, invasion and migration of poorly differentiated BGC823 gastric cancer cells with strong invasive and metastatic abilities. The anti-proliferative and pro-apoptotic effects of Pae on BGC823 cells were verified by Cell Counting kit-8 and Annexin V-fluorescein isothiocyanate/propidium iodide assays. Cell scratch-wound healing and Transwell methods were applied, and it was demonstrated that Pae could exert inhibitory activities on the invasion and migration of BGC823 cells. Furthermore, it was indicated by western blot analysis that Pae could downregulate the protein expression levels of matrix metalloproteinase (MMP)-2 and −9 in a concentration-dependent manner, which may support a novel potential mechanism accounting for its anti-cancer effects on gastric cancer.

microRNA‑145 modulates migration and invasion of bladder cancer cells by targeting N‑cadherin

“…MMPs, known as proteolytic enzymes, were previously reported to be associated with tumor metastasis and invasion (45,46). Additionally, MMP9, an extracellular matrix-degrading enzyme, serves a role in metastasis and invasion of carcinoma cells (47,48). MMP9 was identified as a modulatory target of N-cadherin-dependent signaling (23).…”

<p>Paeonol Inhibits Pancreatic Cancer Cell Migration and Invasion Through the Inhibition of TGF-β1/Smad Signaling and Epithelial-Mesenchymal-Transition</p>

“…25,26 Furthermore, previous studies have shown that Paeonol inhibits invasion and migration of gastric cancer cells by down-regulating the MMPs. 9 However, the anti-metastatic effects of Paeonol, especially in PDAC, lack conclusive evidence. In the absence of other known studies on the anti-migration and anti-invasion activities of Paeonol, we aim to further investigate the activities of Paeonol on migration and invasion and its underlying mechanisms.…”

“…[9][10][11] Previous studies suggested that Paeonol possesses anti-proliferation, apoptosis induction, and cell cycle arresting effects in various types of cancers, such as breast, colorectal, lung, ovarian, gastric cancer, etc. 9,[12][13][14][15][16][17][18][19][20][21] More recent studies reported that Paeonol enhanced paclitaxel-induced apoptosis in breast cancer cells, 20,22 reversed Doxorubicin resistance in liver cancer, 23 and enhanced the radiosensitivity of lung cancer cells. 24 Studies also reported the anti-metastatic activities of Paeonol in human fibrosarcoma, chondrosarcoma and gastric cancer.…”

“…24 Studies also reported the anti-metastatic activities of Paeonol in human fibrosarcoma, chondrosarcoma and gastric cancer. 9,25,26 However, whether Paeonol exerts antimigration and anti-invasion effects in pancreatic cancer cells and the potential underlying mechanisms of action are yet to be elucidated. In the present study, Panc-1 and Capan-1 cell lines are used to study the effects of Paeonol on migration and invasion, and the potential role of TGF-β1-induced EMT as an underlying mechanism of Paeonol is investigated.…”

Cynanchum paniculatum and Its Major Active Constituents for Inflammatory‐Related Diseases: A Review of Traditional Use, Multiple Pathway Modulations, and Clinical Applications

“…In view of CM, the potential anticancer effect of Cynanchum paniculatum can be ascribed to one of the CM traits by clearing wind-damp or removing toxicity. Despite the lack of direct claims to the anticancer properties of Cynanchum paniculatum, the link between anticancer and anti-inflammation can be established after anti-inflammation, anticachectic, antioxidant, antiproliferation, and antiinvasion properties [99][100][101][102][103].…”