Paeoniflorin, the main active constituent of Paeonia lactiflora roots, attenuates bleomycin-induced pulmonary fibrosis in mice by suppressing the synthesis of type I collagen

Ji, Yu; Wang, Ting; Wei, Zhifeng; Lu, Guo-Xun; Jiang, Si-de; Xia, Yufeng; Dai, Yue

doi:10.1016/j.jep.2013.08.017

Cited by 62 publications

(26 citation statements)

References 25 publications

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“…PF exhibits pharmacological activity, including antioxidant, anti-inflammatory and neuroprotective effects, on various types of cells (17)(18)(19) proliferation of annulus fibrosus cells and inhibited FasL-induced apoptosis of annulus fibrosus cells; the results were consistent with a previous study on the cytoprotective effects of PF (20).…”

Section: Discussionsupporting

confidence: 90%

Protective effects of paeoniflorin against FasL-induced apoptosis of intervertebral disc annulus fibrosus cells via Fas-FasL signalling pathway

Chen

Lin

Zheng

et al. 2015

Experimental and Therapeutic Medicine

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Abstract. In the present study, we demonstrate that the degeneration of intervertebral discs is caused by ageing and apoptosis of matrix cells. Apoptosis is as essential as the function of proteoglycan synthesis in assessing the possible degeneration of intervertebral discs; paeoniflorin (PF) induces cytoprotective effects on various types of cells. In this study, the function of PF in inhibiting Fas ligand (FasL)-induced apoptosis in annulus fibrosus cells was assessed, and the correlation between apoptosis and the Fas-FasL pathway was determined. Annulus fibrosus cells were derived from the intervertebral discs of 1-month-old Sprague Dawley rats; the cells were characterised by toluidine blue staining and subjected to apoptosis with FasL. PF was diluted to various concentrations and added to annulus fibrosus cells at various times. The impact of PF and FasL on cell apoptosis of annulus fibrosus cells was determined by flow cytometry. Western blot analysis was performed to determine the protein expression levels of Fas and caspase-3. The percentages of apoptotic annulus fibrosus cells as well as the expression levels of caspase-3 and Fas were significantly reduced following treatment with 208, 20.8 or 2.08 µM PF. PF inhibits the activation of the Fas-FasL signal pathway and decreases FasL-induced apoptosis of annulus fibrosus cells.

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Section: Discussionsupporting

confidence: 90%

Protective effects of paeoniflorin against FasL-induced apoptosis of intervertebral disc annulus fibrosus cells via Fas-FasL signalling pathway

Chen

Lin

Zheng

et al. 2015

Experimental and Therapeutic Medicine

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“…The present study demonstrated that MMP-9 activity of human pancreatic cancer BXPC-3 cells was significantly reduced by paeoniflorin treatment, which is in agreement with the results of Lu et al (20) which indicated paeoniflorin inhibited the tumor invasion and metastasis of human hepatocellular cancer cells via suppression of MMP-9 and ERK. Ji et al (24) reported that paeoniflorin suppressed type I collagen synthesis via the inhibition of MMP-1 mRNA expression.…”

Section: Discussionmentioning

confidence: 99%

Paeoniflorin inhibits human pancreatic cancer cell apoptosis via suppression of MMP-9 and ERK signaling

et al. 2016

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Abstract. Paeoniflorin exhibits anticancer, anti-inflammatory and antioxidation effects, as well as specific pharmacological effects on smooth muscle and the immune, cardiovascular and central nervous systems. The present study aimed to investigate the anticancer effects of paeoniflorin on pancreatic cancer cells and to elucidate the mechanisms by which these effects occur. In the present study, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase assays were performed to assess cell viability and cell cytotoxicity of BXPC-3 human pancreatic cancer cells, respectively. Cellular apoptosis and caspase-3/9 activities were analyzed using an Annexin V-fluorescein isothiocyanate/propidium iodide Apoptosis Detection kit, a DAPI staining assay and colorimetric kits, respectively. Matrix metalloproteinase-9 (MMP-9) and extracellular signal-regulated kinases (ERK) protein expression in BXPC-3 cells were also investigated using gelatin zymography assays and western blot analysis, respectively. In the present study, paeoniflorin was found to inhibit the cell viability and increase cell cytotoxicity of BXPC-3 cells in a dose-and time-dependent manner. In addition, cellular apoptosis, as well as caspase-3 and -9 activity of BXPC-3 cells was increased following paeoniflorin treatment. Notably, paeoniflorin reduced MMP-9 and ERK protein expression in BXPC-3 cells. These results indicate that paeoniflorin exhibits a potential anticancer effect by enhancing human pancreatic cancer cell apoptosis via the suppression of MMP-9 and ERK signaling.

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“…It does appear, based on the findings in this study that PF might be the major phytochemical entity in Paeonia lactiflora and Astragalus membranaceus extracts responsible for the anti-fibrotic bioactivity. This views is supported by the fact that it had similar effects on both pulmonary [5,6] and renal interstitial fibrosis [7].…”

Section: Discussionmentioning

confidence: 81%

“…Paeoniflorin (PF), isolated from Corte moutan, is a major bioactive compound with antiinflammatory and immune-regulatory effects [3,4]. The anti-fibrotic effect of PF has been studied on both pulmonary [5,6] and renal interstitial fibrosis [7]. Studies of the effect of PF'on Schistosomiasis japonica-induced liver fibrosis have also been carried out [8,9].…”

Section: Introductionmentioning

confidence: 99%

Mitigation of TGF-β/Smad signaling pathway-associated liver fibrosis by paeoniflorin

Li¹,

Wei²

2017

Trop. J. Pharm Res

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Paeoniflorin, the main active constituent of Paeonia lactiflora roots, attenuates bleomycin-induced pulmonary fibrosis in mice by suppressing the synthesis of type I collagen

Cited by 62 publications

References 25 publications

Protective effects of paeoniflorin against FasL-induced apoptosis of intervertebral disc annulus fibrosus cells via Fas-FasL signalling pathway

Protective effects of paeoniflorin against FasL-induced apoptosis of intervertebral disc annulus fibrosus cells via Fas-FasL signalling pathway

Paeoniflorin inhibits human pancreatic cancer cell apoptosis via suppression of MMP-9 and ERK signaling

Mitigation of TGF-β/Smad signaling pathway-associated liver fibrosis by paeoniflorin

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