Paeoniflorin protects Schwann cells against high glucose induced oxidative injury by activating Nrf2/ARE pathway and inhibiting apoptosis

Yang, Xinwei; Yao, Weijie; Shi, Haotian; Liu, Haolong; Li, Yangfan; Gao, Yanbin; Liu, Renhui; Xu, Liping

doi:10.1016/j.jep.2016.03.031

Cited by 83 publications

(55 citation statements)

References 35 publications

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“…In our previous study, TLN was found to improve the deficiency of motor nerve conductivity, sensory nerve conductivity and thresholds of thermal perception which were accompanied by lesser demyelination and a milder axonal atrophy in DPN rats 23 . It was also found to induce apoptosis in hyperglycemic media 32 . Based on these previous studies, we endeavored to investigate the effect of TLN on oxidative stress and ER stress in DPN rats and high glucose treated-SCs.…”

Section: Discussionmentioning

confidence: 98%

“…Assay analysis was performed as previously described 32 . Briefly, 20 μL of MTT solution (5 mg/mL) was added and incubated for 4 h at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

“…Table 3) at 4 °C overnight. After washing, cells were incubated with FITC-conjugated goat anti-rabbit IgG (1:200 dilution) at room temperature for 1 h and counterstained with DAPI for 5 min 32, 48 .…”

Section: Methodsmentioning

confidence: 99%

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Tangluoning, a traditional Chinese medicine, attenuates in vivo and in vitro diabetic peripheral neuropathy through modulation of PERK/Nrf2 pathway

Yang

Yao

Liu

et al. 2017

Sci Rep

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Prolonged hyperglycemia-induced oxidative stress and endoplasmic reticulum stress have been demonstrated to play a key role in progression of diabetic peripheral neuropathy (DPN). PERK/ Nrf2 pathway plays a predominant role in oxidative and endoplasmic reticulum (ER) stress which is associated with cell survival. This study examined the modulation of the PERK/Nrf2 pathway and apoptosis by a traditional Chinese medicine Tangluoning (TLN) in streptozotocin-induced DPN rat models and the effects of serum TLN on the PERK/Nrf2 pathway, apoptosis, intracellular reactive oxygen species and mitochondrial membrane potential in Schwann cells cultured in 150 mM glucose. It is found that TLN attenuated oxidative and ER stress and apoptosis through the PERK/Nrf2 pathway by upregulating p-PERK, Nrf2/ARE pathways and downregulating the CHOP-related apoptosis pathways in the experimental DPN models both in vivo and in vitro.

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Section: Discussionmentioning

confidence: 98%

“…Assay analysis was performed as previously described 32 . Briefly, 20 μL of MTT solution (5 mg/mL) was added and incubated for 4 h at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Tangluoning, a traditional Chinese medicine, attenuates in vivo and in vitro diabetic peripheral neuropathy through modulation of PERK/Nrf2 pathway

Yang

Yao

Liu

et al. 2017

Sci Rep

Self Cite

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“…The atractylon in Atractylodes macrocephala Koidz was reported to have strong antioxidative abilities [59, 60]. The Paeoniflorin in Radix Paeoniae Alba targets the Nrf2/ARE signal pathway to inhibit the oxidative stress in the high-glucose-induced Schwann cells by reduction of the amounts of ROS and MDA and by the increased expressions of GST and GPX [61]. Fruits of Fructus Schisandrae Chinensis can improve the oxidative damage in AD by inhibition of RAGE/NF- κ B/MAPK and upregulation of HSP/Beclin [62].…”

Section: Discussionmentioning

confidence: 99%

Recuperating Lung Decoction Attenuates the Oxidative Stress State of Chronic Obstructive Pulmonary Disease by Inhibiting the MAPK/AP‐1 Signal Pathway and Regulating γ‐GCS

Shi

Yan

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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Purpose/Objective. To evaluate the effects of Recuperating Lung Decoction (RLD) on the indices of oxidative stress in a rat model of COPD and detect the indices of the MAPK/AP-1/γ-GCS signal pathway for a further survey of the possible targeting site of RLD. Methods/Materials. The rats of COPD were treated with RLD. The protein levels of glutathione (GSH), oxidized glutathione (GSSG), 8-hydroxy-2-deoxyguanosine (8-OHdG), and 4-hydroxynonenal (4-HNE) were measured. In addition, the levels of key signaling molecules (extracellular signal-regulated kinases [ERK], the c-jun N-terminal kinase [JNKs signal pathway], and p38 MAP kinase [p38MAPK], AP-1 proteins [C-fos, C-jun], and γ-glutamyl-cysteine synthetase [γ-GCS-h]) of the MAPK/AP-1/γ-GCS-h signal pathway were assessed. Results. After treatment, the protein level of GSH and the ratio of GSH/GSSG were increased and the amounts of 8-OHdG and 4-HNE were decreased significantly in lung tissues when compared with the nontreated COPD group. Further results showed that the RLD could effectively inhibit the MAPK pathway by inactivation of p38MAPK and ERK and could also downregulate the AP-1 and the γ-GCS-h genes expressions in both protein and mRNA levels. Conclusion. RLD might improve the state of oxidative stress by downregulation of the expression of γ-GCS-h gene by inhibition of the MAPK/AP-1 pathway, thereafter enhancing the ability of antioxidation in COPD.

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“…Yang et al have reported that this compound can significantly reduce hyperglycemia resulting oxidative stress in Schwann cells. Moreover, paeoniflorin has been shown to stimulated nuclear translocation of the Nrf2 factor and subsequently to upregulate the Nrf2/ARE pathway (Yang et al, ).…”

Section: Neurotoxicitymentioning

confidence: 99%

The neuroprotective activities of natural products through the Nrf2 upregulation

Tavakkoli

Iranshahi

Hasheminezhad

et al. 2019

Phytotherapy Research

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Nuclear factor (erythroid‐derived 2)‐like 2 (Nrf2) is a basic‐region leucine zipper transcription factor that regulates the expression of many Phase II detoxifying/antioxidant enzymes. Many natural products act on the Nrf2 pathway and exhibit their preventive and/or therapeutic effects by activating Nrf2 and subsequently antioxidant enzymes. In this article, we reviewed the research literature that described Nrf2 activation as a mechanism for the neuroprotective effect of natural products. Our results showed that the inhibition of Kelch‐like ECH‐associated protein 1 and the activation of different intracellular kinases that lead to Nrf2 phosphorylation were the main mechanisms of Nrf2 upregulation suggested for these the natural products. Although some natural compounds such as terpenoids and alkaloids were reported to protect the nervous system by Nrf2 activating, flavonoids and phenolic compounds represent the majority of the reported neuroprotective natural substances that activate Nrf2. We also evaluated the physiochemical properties of some of the same substances to predict their oral bioavailability and central nervous system penetration. The results showed that sulforaphane and berberine have good oral bioavailability and would be predicted to cross through blood–brain barrier (BBB) easily, whereas the flavonoids in general would be predicted to be less effective in BBB transition because of their relatively high polar surface area.

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Paeoniflorin protects Schwann cells against high glucose induced oxidative injury by activating Nrf2/ARE pathway and inhibiting apoptosis

Cited by 83 publications

References 35 publications

Tangluoning, a traditional Chinese medicine, attenuates in vivo and in vitro diabetic peripheral neuropathy through modulation of PERK/Nrf2 pathway

Tangluoning, a traditional Chinese medicine, attenuates in vivo and in vitro diabetic peripheral neuropathy through modulation of PERK/Nrf2 pathway

Recuperating Lung Decoction Attenuates the Oxidative Stress State of Chronic Obstructive Pulmonary Disease by Inhibiting the MAPK/AP‐1 Signal Pathway and Regulating γ‐GCS

The neuroprotective activities of natural products through the Nrf2 upregulation

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