Paenibacillus shirakamiensis sp. nov., isolated from the trunk surface of a Japanese oak (Quercus crispula)

Abstract

A novel bacterial strain designated P-1T was isolated from the trunk surface of a Japanese oak (Quercus crispula) growing in the Shirakami Mountains in Japan. Cells of strain P-1T were Gram-stain-negative, ellipsoidal endospore-forming, aerobic, slightly acidophilic rods, 0.8×2–5 µm, and motile by means of peritrichous flagella. Various carbohydrates could be used as growth substrates, but none of the organic acids tested were used. The major cellular fatty acid was anteiso-C15 : 0, which accounted for 64.2 % of the total fatty acids. The major respiratory quinone was menaquinone 7 (MK-7). Strain P-1T contained phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine, four unidentified aminolipids, an unidentified phospholipid and two unidentified polar lipids. Strain P-1T shared the highest 16S rRNA gene sequence similarity with Paenibacillus pini S22T (96.6 %), followed by Paenibacillus chibensis JCM 9905T (96.1 %) and Paenibacillus anaericanus MH21T (95.9 %). The DNA G+C content was 43.9 mol%. These data indicate that strain P-1T represents a novel species within the genus Paenibacillus , for which we propose the name Paenibacillus shirakamiensis sp. nov. The type strain is P-1T (NBRC 109471T = DSM 26806T = KCTC 33126T = CIP 110571T).

Paenibacillus oenotherae sp. nov. and Paenibacillus hemerocallicola sp. nov., isolated from the roots of herbaceous plants

“…The diamino acid in the peptidoglycan is meso -diaminopimelic acid, and the major cellular fatty acid is anteiso-C 15 : 0 (Ash et al , 1993; Shida et al , 1997). Novel species of the genus have been isolated recently from various sources, including compost (Ueda et al , 2013), food (Shimoyama et al , 2014), lake water (Wu et al , 2013), root nodules (Carro et al , 2013, 2014), plants (Kong et al , 2013; Tonouchi et al , 2014), the rhizosphere (Son et al , 2014; Zhang et al , 2013), rock (Guo et al , 2014), sediment (Lee et al , 2013) and soil (Dsouza et al , 2014; Li et al , 2014a, b; Xiang et al , 2014; Yao et al , 2014).…”

Paenibacillus zeisoli sp. nov., isolated from maize-cultivated soil artificially contaminated with cadmium

“…Table 2. Cellular fatty acid compositions of strain 3-5-3 T and type strains of related species Strains: 1, 3-5-3 T (this study); 2, Fontibacillus phaseoli LMG 27589 T (this study); 3, Paenibacillus anaericanus DSM 15890 T (this study); 4, Paenibacillus shirakamiensis P-1 T (data from [9]). Values are percentages of total fatty acids.…”

“…The predominant cellular fatty acid is anteiso-C 15 : 0 . At the time of writing, the genus Paenibacillus comprised more than 200 species and four subspecies in the List of Prokaryotic names with Standing in Nomenclature (LPSN; www.bacterio.net/paenibacillus.html) [6], which are widely distributed in a variety of ecological environments, such as soil [7], water [8], plant material [9, 10], animal material [11], faeces [12] and clinical samples [13]. The isolation source of some species in the genus Paenibacillus was also found to be associated with maize, for example, Paenibacillus brasilensis [14] and Paenibacillus rhizoplanae [15] (from maize rhizosphere), Paenibacillus radicis [16] and Paenibacillus wenxiniae [17] (from maize root), Paenibacillus nebraskensis [18] (from maize root surface), and Paenibacillus zeae [19] (from maize seeds).…”

“…Based on the data from the polyphasic taxonomy study, we conclude that strain 3-5-3 T represents a novel species of the Table 1. Differential characteristics of strain 3-5-3 T and its closely related species of the genus Fontibacillus and Paenibacillus Strains: 1, 3-5-3 T ; 2, Fontibacillus phaseoli LMG 27589 T ; 3, Paenibacillus anaericanus DSM 15890 T ; 4, Paenibacillus shirakamiensis P-1 T (data from [9]). +, Positive; À, negative; w, weakly positive.…”

Paenibacillus qingshengii sp. nov., isolated from a lead–zinc tailing

“…Based on 16S rRNA gene sequence analysis, Ash et al (1993/1994) separated the genus Paenibacillus , which is a member of a monophyletic group of endospore-forming bacteria in the family Paenibacillaceae , from the genus Bacillus . Species of the genus Paenibacillus have been isolated from diverse environmental habitats (Kishore et al , 2010; Baik et al , 2011; Kim et al , 2012; Zhou et al , 2012; Kong et al , 2013; Lee et al , 2013; Zhang et al , 2013; Guo et al , 2014; Tonouchi et al , 2014). At the time of writing, the genus Paenibacillus comprised 159 species and four subspecies (http://www.bacterio.net/paenibacillus.html).…”