Paenibacillus polymyxa Purified Bacteriocin To Control Campylobacter jejuni in Chickens

Stern, Norman J.; Svetoch, E. A.; Eruslanov, Boris V.; Kovalev, Yuri N.; Volodina, L I; Perelygin, Vladimir V.; Mitsevich, E. V.; Mitsevich, Irina P.; Levchuk, Vladimir P.

doi:10.4315/0362-028x-68.7.1450

Cited by 100 publications

(62 citation statements)

References 18 publications

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“…Another example is the recent discovery of a class IIa bacteriocin (molecular mass, 3,864 Da) from P. polymyxa NRRL-B-30509, which has been used for the control of Campylobacter spp. in poultry (34,35). However, the coproduction of polymyxin and a lantibiotic by natural isolates has never been reported.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Identification of a Paenibacillus polymyxa Strain That Coproduces a Novel Lantibiotic and Polymyxin

He¹,

Kışla²,

Zhang³

et al. 2007

Appl Environ Microbiol

191

146

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A new bacterial strain, displaying potent antimicrobial properties against gram-negative and gram-positive pathogenic bacteria, was isolated from food. Based on its phenotypical and biochemical properties as well as its 16S rRNA gene sequence, the bacterium was identified as Paenibacillus polymyxa and it was designated as strain OSY-DF. The antimicrobials produced by this strain were isolated from the fermentation broth and subsequently analyzed by liquid chromatography-mass spectrometry. Two antimicrobials were found: a known antibiotic, polymyxin E1, which is active against gram-negative bacteria, and an unknown 2,983-Da compound showing activity against gram-positive bacteria. The latter was purified to homogeneity, and its antimicrobial potency and proteinaceous nature were confirmed. The antimicrobial peptide, designated paenibacillin, is active against a broad range of food-borne pathogenic and spoilage bacteria, including Bacillus spp., Clostridium sporogenes, Lactobacillus spp., Lactococcus lactis, Leuconostoc mesenteroides, Listeria spp., Pediococcus cerevisiae, Staphylococcus aureus, and Streptococcus agalactiae. Furthermore, it possesses the physico-chemical properties of an ideal antimicrobial agent in terms of water solubility, thermal resistance, and stability against acid/alkali (pH 2.0 to 9.0) treatment. Edman degradation, mass spectroscopy, and nuclear magnetic resonance were used to sequence native and chemically modified paenibacillin. While details of the tentative sequence need to be elucidated in future work, the peptide was unequivocally characterized as a novel lantibiotic, with a high degree of posttranslational modifications. The coproduction of polymyxin E1 and a lantibiotic is a finding that has not been reported earlier. The new strain and associated peptide are potentially useful in food and medical applications.

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Section: Discussionmentioning

confidence: 99%

Isolation and Identification of a Paenibacillus polymyxa Strain That Coproduces a Novel Lantibiotic and Polymyxin

He¹,

Kışla²,

Zhang³

et al. 2007

Appl Environ Microbiol

191

146

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“…P. polymyxa also produces pyrazine metabolites which was stimulated by valine supplementation [48]. In 2005, Stern et al [49] evaluated anti-Campylobacter activity of three P. polymyxa strains from poultry production environments. In this study, they performed bacteriocin-based treatment to reduce Campylobacter jejuni colonization in poultry.…”

Section: P Polymyxa As Antimicrobial Agentmentioning

confidence: 99%

Ecology and biotechnological potential of Paenibacillus polymyxa: a minireview

2009

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Microbial diversity is a major resource for biotechnological products and processes. Bacteria are the most dominant group of this diversity which produce a wide range of products of industrial signifi cance. Paenibacillus polymyxa (formerly Bacillus polymyxa), a non pathogenic and endospore-forming Bacillus, is one of the most industrially signifi cant facultative anaerobic bacterium. It occurs naturally in soil, rhizosphere and roots of crop plants and in marine sediments. During the last two decades, there has been a growing interest for their ecological and biotechnological importance, despite their limited genomic information. P. polymyxa has a wide range of properties, including nitrogen fi xation, plant growth promotion, soil phosphorus solubilisation and production of exopolysaccharides, hydrolytic enzymes, antibiotics, cytokinin. It also helps in biofl occulation and in the enhancement of soil porosity. In addition, it is known to produce optically active 2,3-butanediol (BDL), a potentially valuable chemical compound from a variety of carbohydrates. The present review article aims to provide an overview of the various roles that these microorganisms play in the environment and their biotechnological potential.

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“…The recombinant Pseudomonas fluorescens P3/pME6863 strain and the derivate P. fluorescens P3/ pME6000 were used as degrader and nondegrader reference strains, respectively (18 (21,23); however, to the best of our knowledge no reports on the AHL degradation ability of this microorganism have been presented so far.…”

mentioning

confidence: 99%

Degradation of N -Acyl- l -Homoserine Lactones by Bacillus cereus in Culture Media and Pork Extract

Medina-Martínez

Uyttendaele

Rajković

et al. 2007

Appl Environ Microbiol

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Degradation of the quorum-sensing signal molecule N-acyl-L-homoserine lactone (AHL) in cocultures was verified with Bacillus cereus and Yersinia enterocolitica in culture medium and in pork extract. Results showed evidence of microbial interaction when the AHL-degrading bacterium and AHL-producing bacterium were cocultured in a food-simulating condition.The term "quorum sensing" has been proposed to describe the ability of bacteria to monitor their own population density and modulate gene expression accordingly (17). This communication system uses chemical signal molecules called autoinducers, which are produced and released by the bacterial cell. Intracellular response occurs when the concentration of autoinducers rises above the minimum concentration threshold. Some gram-negative food-associated bacteria can produce Nacyl-L-homoserine lactones (AHLs) as signal molecules. Examples include Aeromonas hydrophila, Pseudomonas aeruginosa, and Yersinia enterocolitica (11,12,20,24,26). In these bacteria, a relationship between AHL production and expression of some mechanisms such as biofilm formation, motility, and exoprotease production has been described previously (2,15,17,25).The signal molecule AHL is the key factor in this bacterial communication process. Degradation of the molecule prevents its sufficient accumulation in the immediate vicinity of the bacterial cell and will consequently lead to a disruption of the communication system. In several fields, blocking of quorum sensing by degradation of AHL molecules has been proposed as a promising alternative to diminish bacterial virulence (4,5,6,9,18). In food systems quorum sensing may have a role in food spoilage, the growth and/or toxin production of pathogens present in food, biofilm formation, bacteriocin production, virulence responses, etc. A possible role of some bacteria in biological degradation of AHLs has been suggested (5,6,7,10,13,14,19,22). Most of the reports on quorum-sensing degradation have been focused on plant pathogens (5, 6, 18). Although it is well known that the lactonase gene is widely spread among certain strains of food-borne Bacillus spp., no actual data are available on the interaction of mixed populations of AHL-producing and AHL-degrading bacteria in food-simulating conditions.The aim of the present study was to screen for the AHLdegrading capacity of Bacillus cereus and some other current and former Bacillus spp. and to establish whether degradation capacity is noted in the coculture environment. The AHLdegrading capacity of Bacillus sp. strains was evaluated with both synthetic AHL molecules and AHL produced by Y. enterocolitica. The AHL degradation ability was further assessed in one B. cereus strain inoculated in coculture with a Y. enterocolitica strain in culture medium and pork extract. Screening of synthetic-AHL degradation at 30°C. Twenty B. cereus and 29 other current and former Bacillus isolates were screened for their AHL degradation capacities. N-Hexanoyl-L-homoserine lactone (C 6 -HSL) (Biochemika Sigma-Aldrich) and ...

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Paenibacillus polymyxa Purified Bacteriocin To Control Campylobacter jejuni in Chickens

Cited by 100 publications

References 18 publications

Isolation and Identification of a Paenibacillus polymyxa Strain That Coproduces a Novel Lantibiotic and Polymyxin

Isolation and Identification of a Paenibacillus polymyxa Strain That Coproduces a Novel Lantibiotic and Polymyxin

Ecology and biotechnological potential of Paenibacillus polymyxa: a minireview

Degradation of N -Acyl- l -Homoserine Lactones by Bacillus cereus in Culture Media and Pork Extract

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