Paenibacillus polymyxa Associated with the Stingless Bee Melipona scutellaris Produces Antimicrobial Compounds against Entomopathogens

Menegatti, Carla; Melo, Weilan Gomes da Paixão; Carrão, Daniel Blascke; Oliveira, Anderson Rodrigo Moraes de; Nascimento, Fábio Santos do; Lopes, Norberto Peporine; Pupo, Mônica Tallarico

doi:10.1007/s10886-018-1028-z

Cited by 27 publications

(28 citation statements)

References 70 publications

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“…P. alvei is a saprophytic, aerobic bacterium which does not grow in healthy bee larvae, but can establish in diseased honey bee colonies in larval remains [ 58 ]. On the other hand, other members of the genus are associated with beneficial functions in solitary and stingless bee nests [ 52 , 59 ], protecting them from molding and other fungal threats. The here identified Paenibacillus was further taxonomically closely related to P. pabuli, P. amylolyticus , and P. xylanexedens , which are usually considered to be environmental, but are associated with co-infections with other bacteria in vertebrates [ 57 , 60 ].…”

Section: Discussionmentioning

confidence: 99%

“…In our case, the lactobacilli found in high abundances for the deceased larvae might have originated from other bees via flowers [ 30 , 32 , 65 , 68 , 69 ]. They might then thrive on the large amounts of available organic pollen resources in the absence of an Osmia bicornis larvae and its microbial associates, which could both compete with and suppress Lactobacillus in the nest [ 52 , 59 , 70 ].…”

Section: Discussionmentioning

confidence: 99%

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Susceptibility of Red Mason Bee Larvae to Bacterial Threats Due to Microbiome Exchange with Imported Pollen Provisions

Voulgari-Kokota

Steffan‐Dewenter

Keller

2020

Insects

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Solitary bees are subject to a variety of pressures that cause severe population declines. Currently, habitat loss, temperature shifts, agrochemical exposure, and new parasites are identified as major threats. However, knowledge about detrimental bacteria is scarce, although they may disturb natural microbiomes, disturb nest environments, or harm the larvae directly. To address this gap, we investigated 12 Osmia bicornis nests with deceased larvae and 31 nests with healthy larvae from the same localities in a 16S ribosomal RNA (rRNA) gene metabarcoding study. We sampled larvae, pollen provisions, and nest material and then contrasted bacterial community composition and diversity in healthy and deceased nests. Microbiomes of pollen provisions and larvae showed similarities for healthy larvae, whilst this was not the case for deceased individuals. We identified three bacterial taxa assigned to Paenibacillus sp. (closely related to P. pabuli/amylolyticus/xylanexedens), Sporosarcina sp., and Bacillus sp. as indicative for bacterial communities of deceased larvae, as well as Lactobacillus for corresponding pollen provisions. Furthermore, we performed a provisioning experiment, where we fed larvae with untreated and sterilized pollens, as well as sterilized pollens inoculated with a Bacillus sp. isolate from a deceased larva. Untreated larval microbiomes were consistent with that of the pollen provided. Sterilized pollen alone did not lead to acute mortality, while no microbiome was recoverable from the larvae. In the inoculation treatment, we observed that larval microbiomes were dominated by the seeded bacterium, which resulted in enhanced mortality. These results support that larval microbiomes are strongly determined by the pollen provisions. Further, they underline the need for further investigation of the impact of detrimental bacterial acquired via pollens and potential buffering by a diverse pollen provision microbiome in solitary bees.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Susceptibility of Red Mason Bee Larvae to Bacterial Threats Due to Microbiome Exchange with Imported Pollen Provisions

Voulgari-Kokota

Steffan‐Dewenter

Keller

2020

Insects

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“…3), active against the entomopathogenic fungus B. bassiana and bacterium P. larvae. Interestingly, fusaricidins 23 and 24 were also detected directly in the larval food of different sampled colonies, suggesting a beneficial defensive role against pathogens [90]. Adult M. scutellaris bees also carry several actinobacteria in their bodies.…”

Section: Natural Products Mediating Microbial Symbiosis In Stingless mentioning

confidence: 95%

Chemical Ecology in Insect-microbe Interactions in the Neotropics

2020

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Small molecules frequently mediate symbiotic interactions between microorganisms and their hosts. Brazil harbors the highest diversity of insects in the world; however, just recently, efforts have been directed to deciphering the chemical signals involved in the symbioses of microorganisms and social insects. The current scenario of natural products research guided by chemical ecology is discussed in this review. Two groups of social insects have been prioritized in the studies, fungus-farming ants and stingless bees, leading to the identification of natural products involved in defensive and nutritional symbioses. Some of the compounds also present potential pharmaceutical applications as antimicrobials, and this is likely related to their ecological roles. Microbial symbioses in termites and wasps are suggested promising sources of biologically active small molecules. Aspects related to public policies for insect biodiversity preservation are also highlighted.

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“…Stingless bees are the largest group of eusocial bees on the planet, with some 60 genera already described [8], however the knowledge about associated microbiota is still limited. Most of the studies described the isolation of microbial strains [9–15], and some protective roles of associated bacteria are also suggested [9–11].…”

Section: Introductionmentioning

confidence: 99%

Microbial community modulates growth of symbiotic fungus required for stingless bee metamorphosis

et al. 2019

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The Brazilian stingless bee Scaptotrigona depilis requires the brood cells-associated fungus Zygosaccharomyces sp. as steroid source for metamorphosis. Besides the presence of Zygosaccharomyces sp., other fungi inhabit S . depilis brood cells, but their biological functions are unknown. Here we show that Candida sp. and Monascus ruber , isolated from cerumen of S . depilis brood provisions, interact with Zygosaccharomyces sp. and modulate its growth. Candida sp. produces volatile organic compounds (VOCs) that stimulate Zygosacchromyces sp. development. Monascus ruber inhibits Zygosacchromyces sp. growth by producing lovastatin, which blocks steroid biosynthesis. We also observed that in co-cultures M . ruber inhibits Candida sp. through the production of monascin. The modulation of Zygosaccharomyces sp. growth by brood cell-associated fungi suggests their involvement in S . depilis larval development. This tripartite fungal community opens new perspectives in the research of microbial interactions with bees.

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Paenibacillus polymyxa Associated with the Stingless Bee Melipona scutellaris Produces Antimicrobial Compounds against Entomopathogens

Cited by 27 publications

References 70 publications

Susceptibility of Red Mason Bee Larvae to Bacterial Threats Due to Microbiome Exchange with Imported Pollen Provisions

Susceptibility of Red Mason Bee Larvae to Bacterial Threats Due to Microbiome Exchange with Imported Pollen Provisions

Chemical Ecology in Insect-microbe Interactions in the Neotropics

Microbial community modulates growth of symbiotic fungus required for stingless bee metamorphosis

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