Paenibacillus macquariensis subsp. defensor subsp. nov., isolated from boreal soil

Hashizume, Tamotsu; Nakabayashi, Takuto; Hirota, Kikue; Matsuno, Tomonori; Koiwa, Ryuuichi; Fujiu, Seiichi; Saito, Izumi; Tkachenko, Oleg B.; Matsuyama, Hidetoshi; Yumoto, Isao

doi:10.1099/ijs.0.006304-0

Cited by 15 publications

(3 citation statements)

References 31 publications

(22 reference statements)

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“…Interestingly, P. odorifer and P. graminis were originally isolated from plant roots as well as from pasteurized pureed vegetables (13), suggesting that that these organisms are also a potential spoilage concern in nondairy foods. In general, Paenibacillus species have been isolated from a number of environments, such as soil (37,60,67,99), rhizospheres (63,96), aquatic environments (9,10,66,86), and compost (94). Paenibacillus has only recently been recognized as a genus separate from Bacillus (8), and as many new species of Paenibacillus continue to be identified (9, 10, 12, 45, 46, 48-52, 64, 66, 86, 90, 92, 94, 97), it is becoming evident that members of this genus occupy diverse environmental niches.…”

Section: Discussionmentioning

confidence: 99%

Identification and Characterization of Psychrotolerant Sporeformers Associated with Fluid Milk Production and Processing

Ivy

Ranieri

Martin

et al. 2012

Appl Environ Microbiol

140

161

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Psychrotolerant spore-forming bacteria represent a major challenge to the goal of extending the shelf life of pasteurized dairy products. The objective of this study was to identify prominent phylogenetic groups of dairy-associated aerobic sporeformers and to characterize representative isolates for phenotypes relevant to growth in milk. Analysis of sequence data for a 632-nucleotide fragment of rpoB showed that 1,288 dairy-associated isolates (obtained from raw and pasteurized milk and from dairy farm environments) clustered into two major divisions representing (i) the genus Paenibacillus (737 isolates, including the species Paenibacillus odorifer, Paenibacillus graminis, and Paenibacillus amylolyticus sensu lato) and (ii) Bacillus (n ‫؍‬ 467) (e.g., Bacillus licheniformis sensu lato, Bacillus pumilus, Bacillus weihenstephanensis) and genera formerly classified as Bacillus (n ‫؍‬ 84) (e.g., Viridibacillus spp.). When isolates representing the most common rpoB allelic types (ATs) were tested for growth in skim milk broth at 6°C, 6/9 Paenibacillus isolates, but only 2/8 isolates representing Bacillus subtypes, grew >5 log CFU/ml over 21 days. In addition, 38/40 Paenibacillus isolates but only 3/47 Bacillus isolates tested were positive for ␤-galactosidase activity (including some isolates representing Bacillus licheniformis sensu lato, a common dairy-associated clade). Our study confirms that Paenibacillus spp. are the predominant psychrotolerant sporeformers in fluid milk and provides 16S rRNA gene and rpoB subtype data and phenotypic characteristics facilitating the identification of aerobic spore-forming spoilage organisms of concern. These data will be critical for the development of detection methods and control strategies that will reduce the introduction of psychrotolerant sporeformers and extend the shelf life of dairy products.

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Section: Discussionmentioning

confidence: 99%

Identification and Characterization of Psychrotolerant Sporeformers Associated with Fluid Milk Production and Processing

Ivy

Ranieri

Martin

et al. 2012

Appl Environ Microbiol

140

161

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“…As bactérias que não apresentam relatos na literatura de importância médica, totalizaram 10 espécies bacterianas divididas em seis gêneros diferentes, sendo eles: Arthrobacter sp, Bacilluss sp, Corynebacterium sp, Curtobacterium sp, Lysinibacillus sp, Paenibacillus sp. (Shiva et al, 2010;Satomi, 2006;Nakamura, 1998;Hoshino et al, 2009) (TABELA 1). (Dalal, 2008;Meira et al, 2014;Aizawa et al, 2007;Koth et al, 2012;Arenskotteret al, 2004;Brandle et al, 2014;Kim et al, 2004;Costa et al, 2010;Rasigade et al, 2012;;Koksal et al, 2009;Hirzel, 2004;Gozalo et al, 2010;Cui et al, 2013) (tabela 2).…”

Section: Resultsunclassified

Bacterial species and forms of resistance of intestinal parasites found in collectives of the city of Divinópolis - MG

Parreira

Melo²,

Almeida³

et al. 2019

Sci. Elec. Arch.

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_______________________________________________________________________________Resumo Ônibus de transporte público são locais de intenso movimento podendo se constituir como importantes veículos de dispersão de microorganismos e parasitas. Em tal contexto, este trabalho buscou avaliar a diversidade microbiana e parasitológica dentro de ônibus urbanos e periurbanos que operam quatro rotas na cidade de Divinópolis MG. Para o estudo microbiológico foram realizadas 80 coletas de amostras empregando-se swabs estéreis contendo meio de transporte de Stuart, sendo levados ao Laboratório de Microbiologia da UEMG -Divinópolis MG para as análises laboratoriais. Para a pesquisa sobre ovos e cistos de parasitas intestinais foram coletadas 80 amostras empregando-se o método de Graham. Na pesquisa microbiológica foram analisados 1097 UFCmL-1 de bactérias heterotróficas. Por meio da técnica de espectrometria de massas foram identificadas onze espécies potencialmente patogênicas de bactérias, das quais sete foram identificadas como resistentes a antibióticos. Na análise parasitológica um provável cisto de Blastocystis hominis e um ovo semelhante à de Hymenolepis sp. foram identificados. Por meio do uso de cotonetes quatro ovos semelhantes aos de Ascaris sp. e um cisto provável de B. ominomin foram identificados. A prevalência bacteriana e parasitológica foi maior nos ônibus que circulam na rota hospitalar e nas áreas periurbanas. Com base nos resultados obtidos foi possível descrever um perfil até agora desconhecido das bactérias e parasitas presentes em algumas das principais rotas de transporte público da Divinópolis MG. Palavras-chave: Bactérias, Parasitas, Ônibus, Divinópolis MG.Abstract. Public transportation buses are places of intense movement of people, which may constitute important dispersal vehicles of microorganisms and parasites. In such context, this paper sought to assess the microbial and parasitological diversity inside urban and periurban buses that run four routes in Divinópolis MG. For the microbial study, 80 sample collections were made by using a sterile swab containing Stuart transport medium and were taken to the Microbiology Laboratory of the UEMG -Divinópolis MG, in order to perform the laboratorial tests. For search about eggs and cysts of intestinal parasites, 80 samples were collected, out of which 40 were collected by using the Graham method, and 40 were collected by using swab. Two smears of each sample were analyzed. In the microbial research, 1097 CFU mL-1 of heterotrophic bacteria were analyzed. Through mass spectrometry eleven potentially pathogenic species of bacteria were identified out of which seven were identified as antibiotics-resistant. In the parasitological analysis through the Graham method a probable cyst of Blastocystis hominis and an egg similar to those of Hymenolepis sp. were identified. Through the use of swab four eggs similar to those of Ascaris sp. and a probable cyst of B.hominis were also identified. The bacterial and parasitological prevalence was higher on buses that run ...

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“…To confirm the above results, we analysed the rpoB gene, a single-copy gene encoding the β-subunit of RNA polymerase. It has already been reported that sequences of this protein-encoding gene have higher discriminatory power than 16S rRNA gene sequences, and sequence comparison of this gene has proven to be helpful to describe the relationships among closely related taxa (Drancourt et al , 2004; Durak et al , 2006; Adékambi et al , 2008; Hoshino et al , 2009). PCR-mediated amplification of the rpoB gene and sequencing of the purified PCR product were carried out according to Wu et al (2011) with some modifications: primers 1F (5′-TTGRCAGGWCAACTWGTTCA-3′) and 3384R (5′-GAAYGATTCCGGAACRCCTGGTTC-3′), whichwere designed in this study, were used instead of primers 73F and 3306R, respectively.…”

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confidence: 99%

Reclassification of Paenibacillus ginsengisoli as a later heterotypic synonym of Paenibacillus anaericanus

Kim

Lee

2011

International Journal of Systematic and Evolutionary Microbiology

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The type strains of the species Paenibacillus ginsengisoli (KCTC 13931 T ) and Paenibacillus anaericanus (DSM 15890 T ) were compared in order to clarify the taxonomic relationship of the two species. On the basis of 16S rRNA and rpoB gene sequence comparisons, the two strains shared 99.9 and 99.6 % similarity, respectively. The mean DNA-DNA relatedness value was 77 % and the genomic DNA G+C contents were 43.2 and 42.2 mol%, respectively. Phenotypic data, including fatty acid patterns and acid-production, enzyme-activity and substrate-utilization profiles, showed no pronounced differences between the type strains of the two species. These genotypic and phenotypic data suggest that the two taxa constitute a single species. According to Rules 38 and 42 of the Bacteriological Code, they should be united under the name Paenibacillus anaericanus, with the name Paenibacillus ginsengisoli as a later heterotypic synonym.The genus Paenibacillus, type genus of the family Paenibacillaceae (De Vos et al., 2010), accommodates facultatively anaerobic or strictly aerobic, rod-shaped bacteria that have ellipsoidal spores in swollen sporangia, anteiso-C 15 : 0 as the major fatty acid and DNA G+C contents ranging from 36 to 59 mol% (Ash et al., 1993;Shida et al., 1997;De Vos et al., 2009). Paenibacilli are ubiquitous in nature, especially in soil. At the time of writing, the genus Paenibacillus comprises 114 recognized species, of which nearly 70 have been isolated from soil environments including humus, phyllosphere, rhizosphere, soil and sediments (Daane et al., 2002; Euzéby, 2010). The genus Paenibacillus has remained monophyletic with ever-increasing numbers of species, but some tightly clustered taxa share .99.5 % 16S rRNA gene sequence similarity, e.g. Paenibacillus favisporus (Velázquez et al., 2004) and Paenibacillus cineris (Logan et al., 2004), Paenibacillus massiliensis and Paenibacillus panacisoli (Ten et al., 2006), and Paenibacillus anaericanus (Horn et al., 2005) and Paenibacillus ginsengisoli (Lee et al., 2007b), which has led to taxonomic re-evaluation. P. ginsengisoli was described by Lee et al. (2007a) for a novel strain isolated from soil of a ginseng field, and its name was validated on Validation List no. 116 (Lee et al., 2007b). According to the original description, whilst P. ginsengisoli formed a tight phylogenetic clade with P. anaericanus due to the high 16S rRNA gene sequence similarity between them, a DNA-DNA relatedness value of 62 % and several phenotypic characteristics distinguished between the two species. However, the DNA-DNA relatedness value was just below the threshold for species delineation (Wayne et al., 1987) and resequencing of the 16S rRNA genes of the type strains of P. anaericanus and P. ginsengisoli revealed that they share 99.9 % sequence similarity, which raised the possibility of their relatedness at the species level. Subsequently, the taxonomic relationship between the two species was re-evaluated by examining a variety of genotypic and phenotypic criteria. T were used for co...

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Paenibacillus macquariensis subsp. defensor subsp. nov., isolated from boreal soil

Cited by 15 publications

References 31 publications

Identification and Characterization of Psychrotolerant Sporeformers Associated with Fluid Milk Production and Processing

Identification and Characterization of Psychrotolerant Sporeformers Associated with Fluid Milk Production and Processing

Bacterial species and forms of resistance of intestinal parasites found in collectives of the city of Divinópolis - MG

Reclassification of Paenibacillus ginsengisoli as a later heterotypic synonym of Paenibacillus anaericanus

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