Paenibacillus konkukensis sp. nov., isolated from animal feed

Abstract

A Gram-stain-positive, oxidase- and catalase-positive, aerobic, rod-shaped bacterium, designated strain SK-3146T, was isolated from animal feed. Phylogenetic analysis, based on 16S rRNA gene sequence comparisons, revealed that the strain formed a distinct lineage within the genus Paenibacillus that was closely related to Paenibacillusyunnanensis JCM 30953T (98.6 %), Paenibacillusvulneris CCUG 53270T (98.0 %) and Paenibacilluschinjuensis DSM 15045T (96.9 %). Cells were non-motile, endospore-forming and formed milky colonies on NA and R2A agar media. Growth of strain SK-3146T occurred at temperatures of 18-45 °C, at pH 6.0-9.5 and between 0.5-3.0 % NaCl (w/v). The major menaquinone was MK-7, with lesser amounts of MK-6 present. The cell wall peptidoglycan of strain SK-3146T contained meso-diaminopimelic acid. The major fatty acids were anteiso-C15 : 0 and iso-C16 : 0. The major polar lipids were diphosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 53.8 mol% and the DNA-DNA hybridization relatedness values between strain SK-3146T and P.yunnanensis JCM 30953T and P.vulneris CCUG 53270T were 26.13±0.8 % and 38.7±0.6 %, respectively. The phenotypic, phylogenetic and chemotaxonomic results indicate that strain SK-3146T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus konkukensis sp. nov. is proposed. The type strain is SK-3146T (=KACC 18876T=LMG 29568T).

Paenibacillus Sinensis sp. nov., a Nitrogen-fixing Species Isolated from Plant Rhizospheres

“…Genomes of strains HN-1 T and 39 were approximately 6.32 and 6.45 Mbp, respectively. The DNA G + C content of the strains HN-1 T and 39 were 53.36 and 52.99 mol%, respectively, which is within the range 39-59 mol% reported for the members of genus Paenibacillus (Im et al 2017). The total number of protein coding genes in HN-1 T and 39 were 5631 and 5782, respectively.…”

Potential Probiotic Acceptability of a Novel Strain of Paenibacillus konkukensis SK 3146 and Its Dietary Effects on Growth Performance, Intestinal Microbiota, and Meat Quality in Broilers

“…In addition, probiotic feeding of P. xylanexedens , which secretes enzymes such as D-cellulose, D-fructose, D-galactose, α-D-glucose, lactose, and maltose as actively as Bacillus species, improved the BW gain and FCR in broilers under normal feeding environment [ 17 ] or challenged conditions [ 18 ]. Similarly, P. konkukensis , isolated and analyzed for complete genome sequence in our previous studies, has DNA sequences encoding various types of enzymes such as β-glucosidase, cellulase, xylanase, and protease, which can degrade various carbohydrates and anti-nutritional factors [ 23 , 24 ]. However, in this study, P. konkukensis had no measured effects on the growth parameters.…”

“…In our previous studies [ 23 , 24 ], P. konkukensis sp. nov. SK-3146 T (=KACC 18876 T = LMG 29568 T ) was isolated from animal feed and completely sequenced.…”

Paenibacillus zeisoli sp. nov., isolated from maize-cultivated soil artificially contaminated with cadmium

“…DPG, PG and PE were found to be the major polar lipids in most of type strains of the genus Paenibacillus . PME was also reported to be one of the major polar lipids in a few species of the genus Paenibacillus such as Paenibacillus konkukensis [42], Paenibacillus vulneris [43], Paenibacillus lemnae [44], Paenibacillus medicaginis [45], Paenibacillus terreus [7] and Paenibacillus pueri [46], but not found in the species of the genus Fontibacillus . Other polar lipids including unidentified glycolipid, aminolipid, phospholipid, phosphoaminolipid and lipids were also found as a small amount of lipids in type strains of the genus Paenibacillus .…”