Paenibacillus campinasensis BL11: A wood material-utilizing bacterial strain isolated from black liquor

Ko, Chun-Han; Chen, Wen-Luen; Tsai, Chung-Hong; Jane, Wann‐Neng; Chia-Chen, Liu; Tu, Jenn

doi:10.1016/j.biortech.2006.09.034

Cited by 60 publications

(43 citation statements)

References 21 publications

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“…However, it was higher than 5.5 kDa as observed by Bastawde (4). In contrast, some workers documented a higher molecular weight than that observed in the present study (5,19,20,38,39). Low molecular weight xylanases are preferred for commercial application in paper and pulp industry as they penetrate throughout the pulp fibres more effectively to hydrolyze xylan making the fiber structure more permeable to extraction of lignin by chemicals (21).…”

Section: Molecular Weight Determinationcontrasting

confidence: 54%

One-step purification and characterization of cellulase-free xylanase produced by alkalophilic Bacillus subtilis ash

Sanghi¹,

Garg²,

Gupta³

et al. 2010

Braz. J. Microbiol.

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The present study describes the one-step purification and characterization of an extracellular cellulase-free xylanase from a newly isolated alkalophilic and moderately thermophilic strain of Bacillus subtilis ASH.Xylanase was purified to homogeneity by 10.5-fold with ~43% recovery using ion-exchange chromatography through CM-Sephadex C-50. The purified enzyme revealed a single band on SDS-PAGE gel with a molecular mass of 23 kDa. It showed an optimum pH at 7.0 and was stable over the pH range 6.0-9.0. The optimum temperature for enzyme activity was 55 °C. The purified xylanase did not lose any activity up to 45 ºC, however, it retained 80% and 51% of its activity after pre-incubation at 55 ºC and 60 ºC, respectively. The enzyme obeyed Michaelis-Menton kinetics towards birch wood xylan with apparent K m 3.33 mg/ml and V max 100 IU/ml. The enzyme was strongly inhibited by Hg 2+ and Cu 2+ while enhanced by Co 2+ and Mn 2+. The purified enzyme could be stored at 4 ºC for six weeks without any loss of catalytic activity. The faster and economical purification of the cellulase-free xylanase from B. subtilis ASH by onestep procedure together with its appreciable stability at high temperature and alkaline pH makes it potentially effective for industrial applications.

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Section: Molecular Weight Determinationcontrasting

confidence: 54%

One-step purification and characterization of cellulase-free xylanase produced by alkalophilic Bacillus subtilis ash

Sanghi¹,

Garg²,

Gupta³

et al. 2010

Braz. J. Microbiol.

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“…Optimal growth is around neutral pH at 55 0 C. The physiological properties of this strain and the vast number of free glycosyl hydrolases produced, give this strain potential for use in bio-refining industry. (Ko et al ., 2007).…”

Section: Introductionmentioning

confidence: 99%

Cultural Conditions Necessary for Optimal Cellulase Yield by Cellulolytic Bacterial Organisms as They Relate to Residual Sugars Released in Broth Medium

Otajevwo¹,

Aluyi²

2011

MAS

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A total of 115 samples made up of 42 (36.5%) rumen fluid, 36 (31.3%) cowdung and 37 (32.2%) soil samples were collected with the aid of sterile swab sticks except for rumen fluid samples which were collected by use of stomach tubes inserted into mouths of cows and by suction, liquor was collected into prewarmed thermo flasks under continuous flushing with carbondioxide. Soil samples were collected into sterile universal containers. All samples were obtained from abattoirs situated at three locations in Benin City, Nigeria. Samples were investigated for cellulolytic bacteria by Filter Paper Yeast Mineral broth method. Cellulase production was assayed by Carboxymethyl cellulose submerged broth culture while residual sugar yield and other cellulolytic activities were determined by 3, 5 -Dinitrosalicylic acid, Filter Paper, Microcrystalline and Viscometric methods. Cellullolytic bacterial organisms isolated from both soil and rumen fluids were Bacillus subtilis, Clostridium cellobioparum and Clostridium thermocellum. Pseudomonas aeruginosa was isolated from both soil and cowdung samples while Erwinia spp was obtained from both rumen fluid and cowdung samples. Bacillus circulans and Serratia spp were obtained from soil samples only. Clostridium thermocellum and Erwinia spp produced the highest and lowest cellulase yields respectively. All isolates at 40 o C and pH 6, recorded optimal sugar yields in culture broth of which Clostridium thermocellum recorded the highest. Lowest yields were recorded at 30 o C and pH 3 although there was significant difference in individual yields (P < 0.05). Clostridium thermocellum recorded optimal cellulolytic activities at 50 o C and pH 6. All isolates attained optimal cellulolytic activities at 32.6 ± 6.2 o C and pH 6.29 ± 0.9 with other broth cultural conditions kept constant. Implications of these findings are discussed.

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“…1.). Paenibacillus genus contains some species such as P. campinasensis BL11 and P. curdlanolyticus B-6 that were reported to have the ability to degrade lignocellulosic materials [42,43], or P. polymyxa BEb-40 which was reported as a promising bacterial strain for the production of endoglucanases, with possibilities of application in the breakdown of lignocellulosic biomass [44]. However, P. illinoisensis has not been reported as having the ability to hydrolyze different lignocellulosic materials.…”

Section: Asian Journal Of Applied Sciences (Issn: 2321 -0893)mentioning

confidence: 99%

Saccharification of Lignocellulosic Materials by Cellulolytic and Xylanolytic <i>Paenibacillus illioisensis </i>CX11

Ahmed

McKay

2017

AJAS

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The utilization of lignocellulosic materials to produce a variety of building blocks (e.g. fermentable sugars) is an interesting alternative approach to meeting the growing demand for high value chemicals. Cellulose and hemicellulose can be hydrolyzed by cellulase and xylanase enzymes into their respective building blocks (hexoses and pentoses), which can later be converted into the targeted compounds. The aim of this study was to test the ability of Paenibacillus illinoisensis CX11 to saccharify different lignocellulosic materials, and to determine its ability to produce cellulolytic and xylanolytic enzymes for possible use in converting lignocellulosic materials into their respective fermentable sugars. The ability of P. illinoisensis CX11 to produce CMCase, xylanase, FPase, and avicelase was tested using SSF of corn stalk. Furthermore, the ability of P. illinoisensis CX11 to saccharify lignocellulosic materials was tested using corn stalk, wheat bran, sawdust, and corn cob. The amount of reducing sugars released from the saccharification of lignocellulosic materials was determined by the 3,5-dinitro-salicylic acid (DNS) method. Obtained results showed that P. illinoisensis CX11 can produce CMCase (400.12 Â± 1.23 U/L), xylanase (385.57 Â± 2.25 U/L), FPase (266.93 Â± 2.22 U/L), avicelase (187.85 Â± 2.22 U/L) and extracellular protein (4.56 Â± 0.14 mg/L). Moreover, P. illinoisensis CX11 showed an ability to saccharify lignocellulosic materials. These findings confirm that P. illinoisensis CX11 has the ability to produce cellulolytic and xylanolytic enzymes, and to hydrolyze different lignocellulosic materials into fermentable sugars. Therefore, this study concludes that P. illinoisensis CX11 can be considered a good source of cellulase and xylanase enzymes to saccharify different lignocellulosic materials.

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Paenibacillus campinasensis BL11: A wood material-utilizing bacterial strain isolated from black liquor

Cited by 60 publications

References 21 publications

One-step purification and characterization of cellulase-free xylanase produced by alkalophilic Bacillus subtilis ash

One-step purification and characterization of cellulase-free xylanase produced by alkalophilic Bacillus subtilis ash

Cultural Conditions Necessary for Optimal Cellulase Yield by Cellulolytic Bacterial Organisms as They Relate to Residual Sugars Released in Broth Medium

Saccharification of Lignocellulosic Materials by Cellulolytic and Xylanolytic <i>Paenibacillus illioisensis </i>CX11

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