Paenalcaligenes suwonensis sp. nov., isolated from spent mushroom compost

Abstract

A bacterial strain, ABC02-12 T , was isolated from spent mushroom compost, a waste product of button mushroom cultivation. Cells of the strain were Gram-stain-negative, catalase-and oxidasepositive, non-spore-forming, aerobic flagellated rods. Paenalcaligenes hermetiae KBL009 T . The quinone system was ubiquinone Q-8 with minor amounts of Q-7. The major fatty acids (.5 % of total fatty acids) were C 16 : 0 , C 16 : 1 v6c and/or C 16 : 1 v7c (summed feature 3), C 18 : 1 v7c and/or C 18 : 1 v6c (summed feature 8), C 17 : 0 cyclo, and iso-C 16 : 1 I, C 14 : 0 3-OH and/or an unknown fatty acid (summed feature 2). The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unknown aminolipid. Putrescine was the principal polyamine, with small amounts of 2-hydroxyputrescine and cadaverine. On the basis of the evidence presented in this study, strain T is a representative of a novel species within the genus Paenalcaligenes, for which the name Paenalcaligenes suwonensis sp. nov. is proposed. The type strain is ABC02-12 T (5KACC 16537 T 5NBRC 108927 T ).The genus Paenalcaligenes was proposed as a member of the family Alcaligenaceae belonging to the class Betaproteobacteria (Kämpfer et al., 2010). The type species of the genus, Paenalcaligenes hominis, was isolated from human blood. Later, the name Paenalcaligenes hermetiae was proposed for an isolate from the larval gut of Hermetia illucens (Lee et al., 2013). The genus was characterized as comprising Gramstain-negative, motile, short rods. Chemotaxonomically, members of the genus contained ubiquinone-8 as the predominant isoprenoid quinone and C 16 : 0 , C 17 : 0 cyclo, summed feature 2 (iso-C 16 : 1 I and/or C 14 : 0 3-OH) and summed feature 3 (C 16 : 1 v7c and/or iso-C 15 : 0 2-OH) as the major fatty acids. The polar lipids present were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, aminolipid and several polar lipids. Putrescine was the major polyamine; spermidine, spermine and cadaverine were the minor ones, and 2-hydroxyputrescine was present in trace amounts (Kämpfer et al., 2010;Lee et al., 2013).In Korea, button mushrooms (Agaricus bisporus) were cultivated on a medium mainly composed of rice straw amended with animal manure and gypsum. Spent mushroom compost, which was a waste product of button mushroom cultivation, was collected from a mushroom cultivation facility in Suwon, Republic of Korea. In the course of a study on cultivable bacteria from spent mushroom compost, one bacterial strain, designated ABC02-12 T , was isolated using a plating technique on R2A agar (BD) at 28 u C and then subjected to a taxonomic investigation using a polyphasic approach.Gram staining was determined using heat-fixed liquid cultures and the Difco Gram staining kit, according to the manufacturer's instructions. The morphology of cells and Abbreviation: AL, unknown aminolipid.

Complete Genome Sequence of a Paenalcaligenes hominis Strain Isolated from a Paraplegic Patient with Neurogenic Bladder Using Single-Molecule Real-Time Sequencing Technology

“…It was first described in 2010 on the basis of a strain isolated from the blood culture of an 85-year-old man (1). Currently, three species are recognized: P. hominis (1), P. hermetiae (2), and P. suwonensis (3). Due to the lack of whole-genome sequences in the NCBI database from any members of the genus Paenalcaligenes , we sequenced a P. hominis isolate.…”

Draft Genome Sequence of Clinical Isolate Alcaligenaceae sp. Strain 429

“…However, 16S rRNA gene sequencing performed by our group indicated that the isolate was more closely related to another member of the Alcaligenaceae family, Paenalcaligenes suwonensis. Paenalcaligenes species have been isolated from both the environment (1) and patients (2, 3), and only one genome has been sequenced for the genus to date (P. hominis).…”

Paenalcaligenes niemegkensis sp. nov., a novel species of the family Alcaligenaceae isolated from plastic waste

“…The type species Paenalcaligenes hominis was isolated from human blood [4]. Two additional species were then proposed: Paenalcaligenes hermetiae , isolated from the larval gut of Hermetia illucens [5], and Paenalcaligenes suwonensis , isolated from spent mushroom compost [6]. So far, species within the genus are Gram-stain negative, short rods, catalase- and oxidase-positive, and mainly showing an aerobic respiratory metabolism.…”