“…Following the protocols described by Rajal et al [43] and Boxus et al [44] , bovine respiratory syncytial virus (Inforce™ 3) (Zoetis, US) RT-qPCR reactions were performed to evaluate the recovery of concentration methods. A normalization step was performed according to previous studies by our research group [17] , [45] . Further, the wastewater samples were spiked with plasmids (pET28a) to determine whether the nucleic acid could be recovered from wastewater.…”