PAD1 and FDC1 are essential for the decarboxylation of phenylacrylic acids in Saccharomyces cerevisiae

Mukai, Nobuhiko; Masaki, Kazuo; Fujii, Tsutomu; Kawamukai, Makoto; Iefuji, Haruyuki

doi:10.1016/j.jbiosc.2009.11.011

Cited by 175 publications

(154 citation statements)

References 22 publications

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“…The decarboxylation ability was only observed in strains that exhibited high transcription levels of both PAD1 and FDC1. This result might suggest that both functional PAD1 and FDC1 are required in this process, which is consistent with previous research (6). Normal transcription of PAD1 was observed in both ferulic acid decarboxylation-active and -inactive strains, indicating that PAD1p was not the direct functional enzyme.…”

Section: Discussionsupporting

confidence: 92%

“…In 1990, Meaden and Taylor (14) cloned a functional gene which endowed yeasts with the ability to decarboxylate phenolic acid. Mukai et al (6) confirmed that both PAD1 and FDC1 are essential for the decarboxylation of ferulic acids in Saccharomyces cerevisiae. Clausen et al (15) reported that PAD1 contributes to cinnamic acid decarboxylation in S. cerevisiae and the introduction of PAD1 into mutants that lacked PADp activity allowed the recovery of this biological activity.…”

Section: Introductionmentioning

confidence: 94%

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Single nucleotide polymorphisms and transcription analysis of genes involved in ferulic acid decarboxylation among different beer yeasts

Chen¹,

Dong²,

Yin³

et al. 2015

J. Inst. Brew.

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The compound 4-vinylguaiacol, which is produced by the decarboxylation of ferulic acid in certain yeasts, is an important flavour compound in the brewing industry. This decarboxylation ability varies significantly in different beer yeasts and most strains possessing this ability are classified as ale yeasts. In this study, both lager and ale strains were used for fermentation tests and the decarboxylation ability was quantified. Two lager and three ale strains were identified with high ferulic acid decarboxylation activity. Single nucleotide polymorphism (SNP) analysis of PAD1 and FDC1 was conducted by gene sequencing and alignment. In addition, transcription analysis of the two genes was also performed with a GeXP genetic analysis system. A positive relationship between SNPs and ferulic acid decarboxylation activity was determined, and the premature termination codon introduced by base substitution and insertion resulted in the loss of this ability in beer yeasts, whereas other SNPs had a relatively limited influence on it. The GeXP results revealed that genes containing nonsense mutations exhibited low transcription levels, which could be explained by nonsense-mediated mRNA decay. These findings will further the understanding of the relationship between SNPs and biological traits, and will hopefully provide a promising strain selection method for industrial yeasts based on SNP markers.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 94%

Single nucleotide polymorphisms and transcription analysis of genes involved in ferulic acid decarboxylation among different beer yeasts

Chen¹,

Dong²,

Yin³

et al. 2015

J. Inst. Brew.

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“…1. The observation that the ylr290c⌬ mutant still produces small amounts of Q 6 is not fully consistent with this hypothesis; however, there may be redundant decarboxylases capable of bypassing the ylr290c⌬ mutant such as Pad1 and Fdc1, two phenylacrylic acid decarboxylases with homology to the E. coli Q biosynthetic decarboxylases UbiD and UbiX (70,71). Expression of yeast PAD1 in an E. coli ubiX mutant restored Q 8 synthesis; however, yeast mutants lacking either PAD1, FDC1, or both produce wild-type levels of Q 6 (71,72), potentially because of their functions as redundant decarboxylases.…”

Section: Discussionmentioning

confidence: 98%

Identification of Coq11, a New Coenzyme Q Biosynthetic Protein in the CoQ-Synthome in Saccharomyces cerevisiae

Allan¹,

Awad²,

Johnson³

et al. 2015

Journal of Biological Chemistry

131

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Background: Yeast synthesizes coenzyme Q via a macromolecular protein complex. Results: The Q biosynthetic complex includes Coq8 and the uncharacterized protein YLR290C; the ylr290c⌬ mutant exhibits impaired Q synthesis. Conclusion: YLR290C (Coq11) is a novel protein required for efficient yeast Q biosynthesis. Significance: Discovery and characterization of yeast Coq biosynthetic proteins leads to an improved understanding of coenzyme Q biosynthesis and regulation.

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“…We do not know whether EPH1 arrived in the wine strains directly from S. paradoxus or from a separate horizontal transfer event. The third S. paradoxus introgression event is only seen in the Brazilian fuel yeast BG1 and encompasses a region of ;4 kb containing the STL1 gene (involved in glycerol transport) (Tulha et al 2010) and the PAD1 and FDC1 genes (both phenolic acid decarboxylases) (Mukai et al 2010). STL1 is expressed in osmotic shock conditions (likely to be experienced in sugar-cane juice), and it is also likely that detoxifying phenolic acids may be important in this environment.…”

Section: Interspecific Hybridization and Introgressionmentioning

confidence: 99%

Analysis of the Saccharomyces cerevisiae pan-genome reveals a pool of copy number variants distributed in diverse yeast strains from differing industrial environments

Dunn¹,

Richter²,

Kvitek³

et al. 2012

Genome Res.

201

182

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Although the budding yeast Saccharomyces cerevisiae is arguably one of the most well-studied organisms on earth, the genome-wide variation within this species—i.e., its “pan-genome”—has been less explored. We created a multispecies microarray platform containing probes covering the genomes of several Saccharomyces species: S. cerevisiae, including regions not found in the standard laboratory S288c strain, as well as the mitochondrial and 2-μm circle genomes–plus S. paradoxus, S. mikatae, S. kudriavzevii, S. uvarum, S. kluyveri, and S. castellii. We performed array-Comparative Genomic Hybridization (aCGH) on 83 different S. cerevisiae strains collected across a wide range of habitats; of these, 69 were commercial wine strains, while the remaining 14 were from a diverse set of other industrial and natural environments. We observed interspecific hybridization events, introgression events, and pervasive copy number variation (CNV) in all but a few of the strains. These CNVs were distributed throughout the strains such that they did not produce any clear phylogeny, suggesting extensive mating in both industrial and wild strains. To validate our results and to determine whether apparently similar introgressions and CNVs were identical by descent or recurrent, we also performed whole-genome sequencing on nine of these strains. These data may help pinpoint genomic regions involved in adaptation to different industrial milieus, as well as shed light on the course of domestication of S. cerevisiae.

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PAD1 and FDC1 are essential for the decarboxylation of phenylacrylic acids in Saccharomyces cerevisiae

Cited by 175 publications

References 22 publications

Single nucleotide polymorphisms and transcription analysis of genes involved in ferulic acid decarboxylation among different beer yeasts

Single nucleotide polymorphisms and transcription analysis of genes involved in ferulic acid decarboxylation among different beer yeasts

Identification of Coq11, a New Coenzyme Q Biosynthetic Protein in the CoQ-Synthome in Saccharomyces cerevisiae

Analysis of the Saccharomyces cerevisiae pan-genome reveals a pool of copy number variants distributed in diverse yeast strains from differing industrial environments

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