Paclobutrazol-induced Leaf, Stem, and Root Anatomical Modifications in Potato

Abstract

<jats:p>Potato (<jats:italic>Solanum tuberosum</jats:italic> L.) treatment with paclobutrazol resulted in short and compact plants having dark green and thicker leaves, and wider stem and root diameters. Investigating the underlying anatomical modifications in response to the treatment was the objective of the study. Plants of potato cultivar BP 1 were treated with 0, 45.0, 67.5, and 90.0 mg paclobutrazol per plant as a foliar spray. A month after treatment leaf, stem and root materials were taken from the control and plants treated with 67.5 mg paclobutrazol, and histological observations were made using light microscope. Leaves of treated plants showed an increased chlorophyll <jats:italic>a</jats:italic> and <jats:italic>b</jats:italic> contents, thicker epicuticular wax layer, elongated and thicker epidermal, palisade and spongy mesophyll cells. paclobutrazol increased stem diameter by about 58% due to induction of thicker cortex, larger vascular bundles, and wider pith diameter associated with larger pith cells. Widening the cortex and the induction of more secondary xylem vessels in response to paclobutrazol treatment increased the root diameter by about 52%. Paclobutrazol treatment remarkably increased the accumulation of starch granules in the stem pith cells and cortical cells of the stem and root. This study is similar to the other relevant studies in reporting an increased leaf thickness, and stem and root diameters; however, most of the underlying anatomical modifications described above have not been reported previously.</jats:p>

Tiba Induced Morphological and Root Anatomical Changes in Lentil

“…Similar results of increase and decrease in leaf pigments due to TIBA treatment have been reported in soybean (Jahan and Khan, 2014). This result is also in agreement with the findings of Tsegaw et al (2005). Root length and root diameter were affected in response to TIBA treatments (Table 1 and Fig.…”

“…Similar results of decrease in plant height due to TIBA treatment have been reported in soybean (Jahan and Khan, 2014) and mungbean (Adam and Jahan, 2014). Tsegaw et al (2005) reported thicker stems due to growth retardant PBZ. Number of branches and leaves per plant showed both increase and decrease due to TIBA treatments over and from the control.…”

“…Increasing root diameter in chrysanthemum may be due to an increase in the number of rows and diameter of cortical cells (Burrows et al 1992). Tsegaw et al (2005) reported increase in the number of secondary xylem vessels and 52% increase in root thickness following growth retardant application. Krause (1971) reported production of small vessels in soybean following TIBA application.…”

Effects of 2, 3, 5-triiodobenzoic acid on the anatomical changes of chickpea var. BARI Chola-7

“…1A, B) in diameter of the stem compared to the control though pith diameter showed a marginal decrease. Similar results were also reported by Berova and Zlatev (9) in tomato and by Tsegaw et al (10) in potato. Due to 20 ppm TIBA, significant changes occurred in the number and size of xylem vessels.…”

“…Increased root diameter following growth retarding chemical application was also reported in other plants, viz. soybean (14) and potato (10) . Burrows et al (15) found reduced stem diameter but roots with increased diameter in chrysanthemum due to similar growth retarding chemical PBZ.…”