Paclitaxel-incorporated nanoparticles of hydrophobized polysaccharide and their antitumor activity

Lee, Sang Joon; Hong, Gun-Young; Jeong, Young‐Il; Kang, Mi‐Sun; Oh, Jong-Suk; Song, Chae-Eun; Lee, Hyun Chul

doi:10.1016/j.ijpharm.2012.04.048

Cited by 56 publications

(20 citation statements)

References 32 publications

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“…Nevertheless, although performed at different conditions, several works report the absence of a cytotoxic effect of pullulan nanoparticles on various types of cells, like human fibroblasts h-TERT BJ1 (Gupta and Gupta, 2004a), monkey fibroblasts COS-7, human embryonic kidney cells HEK 293 (Gupta and Gupta, 2004b) and mouse macrophage-like cells RAW264.7 (Lee et al, 2012).…”

Section: In Vitro Cytotoxicity Study Of Pullulan-based Nanoparticlesmentioning

confidence: 99%

Pullulan-based nanoparticles as carriers for transmucosal protein delivery

Dionísio

Cordeiro

Remuñán‐López

et al. 2013

European Journal of Pharmaceutical Sciences

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Polymeric nanoparticles have revealed very effective in transmucosal delivery of proteins. Polysaccharides are among the most used materials for the production of these carriers, owing to their structural flexibility and propensity to evidence biocompatibility and biodegradability. In parallel, there is a preference for the use of mild methods for their production, in order to prevent protein degradation, ensure lower costs and easier procedures that enable scaling up.In this work we propose the production of pullulan-based nanoparticles by a mild method of polyelectrolyte complexation. As pullulan is a neutral polysaccharide, sulfated and aminated derivatives of the polymer were synthesized to provide pullulan with a charge. These derivatives were then complexed with chitosan and carrageenan, respectively, to produce the nanocarriers. Positively charged nanoparticles of 180-270 nm were obtained, evidencing ability to associate bovine serum albumin, which was selected as model protein. In PBS pH 7.4, pullulan-based nanoparticles were found to have a burst release of 30% of the protein, which maintained up to 24h. Nanoparticle size and zeta potential were preserved upon freeze-drying in the presence of appropriate cryoprotectants. A factorial design was approached to assess the cytotoxicity of raw materials and nanoparticles by the metabolic test MTT. Nanoparticles demonstrated to not cause overt toxicity in a respiratory cell model (Calu-3). Pullulan has, thus, demonstrated to hold potential for the production of nanoparticles with an application in protein delivery.

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Section: In Vitro Cytotoxicity Study Of Pullulan-based Nanoparticlesmentioning

confidence: 99%

Pullulan-based nanoparticles as carriers for transmucosal protein delivery

Dionísio

Cordeiro

Remuñán‐López

et al. 2013

European Journal of Pharmaceutical Sciences

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“…Hydrophobized or hydrophobic polymers can self-assemble in aqueous solution forming nanoparticles. In this context, amphiphilic block copolymers and hydrophobized polymers have been previously synthesized and their structure-function relationships studied (Ding et al, 2011;Lee et al, 2012;Na et al, 2003;Suksiriworapong, Sripha, & Heinze, 2008;Lucero, Claro, Casas, & Jimenez-Castellanos, 2013), dextran (Hassani et al, 2012;Aumelas, Serrero, Durand, Dellacherie, & Leonard, 2007;Hornig, Bunjes, & Heinze, 2009;Nichifor, Lopes, Carpov, & Melo, 1999), pullulan (Lee et al, 2012;Zhang et al, 2009), chondroitin sulfate (Park et al, 2010), chitosan (Hassani et al, 2012;Hwang, Kim, Kwon, & Kim, 2008), hyaluronic acid (Surace et al, 2009), amylose (Lalush, Bar, Zakaria, Eichler, & Shimoni, 2005), heparin (Park et al, 2004) and curdlan (Na, Park, Kim, & Bae, 2000), amylopectin (Lu, Zhang, Wang, & Chen, 2011) alginate (Shi, Zhang, Qi, & Cao, 2012;Pawar & Edgar, 2012) have been used for the formation of self-assembling nanoparticles with a view to their application as drug carriers.…”

Section: Introductionmentioning

confidence: 99%

Self-assembled nanoparticles of acetylated cashew gum: Characterization and evaluation as potential drug carrier

Pitombeira¹,

Neto²,

Silva³

et al. 2015

Carbohydrate Polymers

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“…A notable example of P use is represented by the work of Lee et al . [51], where PTX-incorporated NPs have been synthesized using P acetate. NPs were spherical with a size from 80 nm for empty NPs and up to more than 100 nm for those loaded with PTX.…”

Section: Polysaccharide-based Delivery Systems For Anti-cancer Drugsmentioning

confidence: 99%

Polysaccharides for the Delivery of Antitumor Drugs

et al. 2015

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Among the several delivery materials available so far, polysaccharides represent very attractive molecules as they can undergo a wide range of chemical modifications, are biocompatible, biodegradable, and have low immunogenic properties. Thus, polysaccharides can contribute to significantly overcome the limitation in the use of many types of drugs, including anti-cancer drugs. The use of conventional anti-cancer drugs is hampered by their high toxicity, mostly depending on the indiscriminate targeting of both cancer and normal cells. Additionally, for nucleic acid based drugs (NABDs), an emerging class of drugs with potential anti-cancer value, the practical use is problematic. This mostly depends on their fast degradation in biological fluids and the difficulties to cross cell membranes. Thus, for both classes of drugs, the development of optimal delivery materials is crucial. Here we discuss the possibility of using different kinds of polysaccharides, such as chitosan, hyaluronic acid, dextran, and pullulan, as smart drug delivery materials. We first describe the main features of polysaccharides, then a general overview about the aspects ruling drug release mechanisms and the pharmacokinetic are reported. Finally, notable examples of polysaccharide-based delivery of conventional anti-cancer drugs and NABDs are reported. Whereas additional research is required, the promising results obtained so far, fully justify further efforts, both in terms of economic support and investigations in the field of polysaccharides as drug delivery materials.

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Paclitaxel-incorporated nanoparticles of hydrophobized polysaccharide and their antitumor activity

Cited by 56 publications

References 32 publications

Pullulan-based nanoparticles as carriers for transmucosal protein delivery

Pullulan-based nanoparticles as carriers for transmucosal protein delivery

Self-assembled nanoparticles of acetylated cashew gum: Characterization and evaluation as potential drug carrier

Polysaccharides for the Delivery of Antitumor Drugs

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