Packed column supercritical fluid chromatography of isomeric polypeptide pairs

Patel, M.A.; Riley, Frank; Wang, J.; Lovdahl, Michael; Taylor, Larry T.

doi:10.1016/j.chroma.2011.03.005

Cited by 31 publications

(11 citation statements)

References 12 publications

(12 reference statements)

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“…The inherent speed, efficiency, and versatility of SFC have transformed the perceptions of the technology from novelty to integral tool for the modern labs, especially for those expecting to maximize throughput [5]. Patel et al have successfully adopted packed column SFC for the separation of two-pairs of water soluble peptides of identical mass, composition and charge that differ only in amino acid sequence [6]. Desmortreux et al have studied the separation of furocoumarins of essential oils by SFC with a green mobile phase of CO 2 /ethanol.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

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Adsorption equilibrium of citric acid from supercritical carbon dioxide/ethanol on cyano column

Lü

GuoqingWang

Zhang

et al. 2015

Chinese Journal of Chemical Engineering

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Section: Accepted Manuscriptmentioning

confidence: 99%

“…ACCEPTED MANUSCRIPT 6 this work the experimental data were fitted well by the quadratic Hill model (N=2).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Adsorption equilibrium of citric acid from supercritical carbon dioxide/ethanol on cyano column

Lü

GuoqingWang

Zhang

et al. 2015

Chinese Journal of Chemical Engineering

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“… 109 Even though multiple parameters can be modified in the mobile phase Patel et al (2011) concluded, after analysis of two isomeric peptide pairs with SFC, that choosing the right type of stationary phase is more critical. 111 Stationary phases utilized in SFC include a wide range of different types. There are SFC specific columns e.g.…”

Section: Peptide Analysis and Lipophilicity Determination With Chroma...mentioning

confidence: 99%

A story of peptides, lipophilicity and chromatography – back and forth in time

Erckes¹

2022

RSC Med. Chem.

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“…The characteristics of the mobile phase in SFC can provide for tunable selectivity, although the stationary phase also affects selectivity in SFC. Most of the stationary phases used in SFC are extensions of those used in HPLC, although stationary phases designed for achiral SFC have been explored .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of an amide‐based stationary phase for supercritical fluid chromatography

Borges-Muñoz

Colón

2016

J of Separation Science

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A relatively new stationary phase containing a polar group embedded in a hydrophobic backbone (i.e., ACE® C18-amide) was evaluated for use in supercritical fluid chromatography. The amide-based column was compared with columns packed with bare silica, C18 silica, and a terminal-amide silica phase. The system was held at supercritical pressure and temperature with a mobile phase composition of CO2 and methanol as cosolvent. The linear solvation energy relationship model was used to evaluate the behavior of these stationary phases, relating the retention factor of selected probes to specific chromatographic interactions. A five-component test mixture, consisting of a group of drug-like molecules was separated isocratically. The results show that the C18-amide stationary phase provided a combination of interactions contributing to the retention of the probe compounds. The hydrophobic interactions are favorable; however, the electron donating ability of the embedded amide group shows a large positive interaction. Under the chromatographic conditions used, the C18-amide column was able to provide baseline resolution of all the drug-like probe compounds in a text mixture, while the other columns tested did not.

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Packed column supercritical fluid chromatography of isomeric polypeptide pairs

Cited by 31 publications

References 12 publications

Adsorption equilibrium of citric acid from supercritical carbon dioxide/ethanol on cyano column

Adsorption equilibrium of citric acid from supercritical carbon dioxide/ethanol on cyano column

A story of peptides, lipophilicity and chromatography – back and forth in time

Evaluation of an amide‐based stationary phase for supercritical fluid chromatography

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