The Packed cell volume [PCV] is a measurement of the proportion of blood that is made up of cells. The Packed cell volume [PCV] can be determined by centrifuging heparinized blood in a capillary tube at 10,000 RPM for a few minutes. This separates the blood into the layer. The automated hematology analyzer uses the principle in which the blood is passed between two electrodes through an aperture so narrow that only one cell can pass through at a time. The measurement depends on the number and size of the red blood cell.Hematocrit levels that are high or low indicate a blood disorder. PCV valve is normally 42% to 50% for men and 36% to 45% for women. The aim of our study is to compare the PCV value by manual and automated method. This study is a prospective study, by comparing the packed cell volume by the manual and the automated method using twenty peripheral venous blood samples, which were collected from the patient with an informed consent. It is evident that measuring the packed cell volume by the automated method was found to be more accurate when compared to the manual method.This study depicts a significant outcome in measuring the packed cell volume by the automated method could be more effective in obtaining accurate interpretation.