The current study aimed to compare time spent above 90% V̇O2max (tV̇O2max) during 3 work-matched interval training protocols comprising 8 x 60-second exercise efforts with decreasing, increasing, or constant work rate distribution within each exercise interval. Ten healthy male subjects (age: 27.6 ± 5.0 years; V̇O2max: 3.82 ± 0.52 L•min-1) performed an incremental test to determine V̇O2max and peak power output (Pmax). During visits 2, 3, and 4, three work-matched interval training sessions comprising 8 x 60 s efforts: 60 s active recovery with the power output held constant (100%Pmax; ITCON), decreasing (from 110 to 90%Pmax; ITDEC), or increasing (from 90 to 110%Pmax; ITINC) linearly throughout each work interval. Time sustained above 90% of V̇O2max (tV̇O2max) or HRmax (tHRmax), blood lactate concentrations (BLC) and rating of perceived exertion (RPE) were measured. The tV̇O2max (ITCON: 274 ± 132; ITDEC: 313 ± 102; ITINC: 310 ± 113 s, P = 0.37), tHRmax (ITCON: 396 ± 180; ITDEC: 441 ± 207; ITINC: 390 ± 212 s, P = 0.47), BLC (P = 0.73), and final RPE (P = 0.75) were similar among protocols. In conclusion, work-matched interval training induced similar time near V̇O2max and associated physiological responses regardless of work rate manipulation.