Pachypodol, a flavonol from the leaves of Calycopteris floribunda, inhibits the growth of CaCo 2 colon cancer cell line in vitro

Abstract

Calycopteris floribunda Lam., commonly known as 'goichia lata or goache lata', is a large climbing woody shrub from Bangladesh, and well distributed in a number of other south-east Asian countries. Traditionally, C. floribunda has been used in colic, as an antihelminthic, astringent and carminative, and for the treatment of diarrhoea, dysentery, jaundice and malaria in many countries including Bangladesh. Pachypodol (5,4'-dihydroxy-3,7,3'-trimethoxyflavone) has been isolated from the leaves of C. floribunda by repeated column chromatography on silica gel, and the structure confirmed by spectroscopic means. While the general toxicity of pachypodol was determined by the brine shrimp lethality assay, the cytotoxic potential of this flavonoid has been evaluated by the Promega's CellTiter 96 Non-Radioactive Cell Proliferation Assay using the CaCo-2 colon cancer cell line (IC(50) = 185.6 microM). A summary of the biological activities of pachypodol reported to date is also presented.

Bioactivity of Excoecaria agallocha

“…Previous phytochemical studies on E. agallocha revealed the presence of diterpenoids (ISI database, 2008;Ji-Dong et al, 2007;Li et al, 2007;Wang et al, 2006Kang et al, 2005), triterpenoids (Zou et al, 2006), fl avonoid (Konishi et al, 2003) and phorbole esters (Ericson et al, 1995). As part of our continuing phytochemical and bioactivity studies on Bangladeshi medicinal plants and also in other parts of the world (Alam et al, 2008a,b;Ali et al, 2008;Datta et al, 2007Datta et al, , 2004Saha et al, 2007;Uddin et al, 2007aUddin et al, -c, 2006aUddin et al, ,b, 2005Uddin et al, , 2004Rouf et al, 2006;Haque et al, 2004;Rahman et al, 2004;Delazar et al, 2006;Shoeb et al, 2007;Auzi et al, 2007;Zamani et al, 2007;Razavi et al, 2008), we now report on the neuropharmacological and antibacterial activities, and acute toxicty of the ethanol extract of the bark of E. agallocha in laboratory rodents as well as brine shrimp toxicity.…”

Targeting Inflammatory Pathways by Flavonoids for Prevention and Treatment of Cancer

“…Rutin was shown to induce apoptosis in leukemia WEHI-3 cells implanted in BALB/c mice, which showed a decreased percentage of the Mac-3 marker after rutin treatment, indicating that the differentiation of the precursor of macrophage and T cells was inhibited [141]. Pachypodol (5,4′-dihydroxy-3,7,3′-trimethoxyflavone), a flavonol isolated from the leaves of Calycopteris floribunda, has been found to cytotoxic as evaluated by the Promegaʼs cell titer 96 nonradioactive cell proliferation assay using CaCo-2 colon cancer cells [142].…”

Hepatoprotective Triterpenoid Saponins from <i>Callicarpa nudiflora</i>

“…The structures of known compounds 5-7 were determined as luteolin (5), 13) 5,4′-dihydroxyl-3,7,3′-trimethoxyflavone (6), 14) and caffeic acid (7), 15) respectively, by detailed spectroscopic analysis and comparison of their spectral data with reported values in the literatures.…”