PaCATB, a secreted catalase protecting Podospora anserina against exogenous oxidative stress

Zintel, Sandra; Bernhardt, Dominik; Rogowska-Wrzesińska, Adelina; Osiewacz, Heinz D.

doi:10.18632/aging.100360

Cited by 16 publications

(15 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Catalase activity in cell extracts of the wild type and the PaCatB deletion strain 36 was investigated in vitro by photometric determination of H 2 O 2 decomposition. The analysis of the PaCatB deletion strain verifies that the major activity measured with this method, under the tested conditions, is PaCATB activity (Figure 4C).…”

Section: Resultsmentioning

confidence: 99%

Effect of paraquat-induced oxidative stress on gene expression and aging of the filamentous ascomycete Podospora anserina

Wiemer¹,

Osiewacz

2014

MIC

View full text Add to dashboard Cite

Aging of biological systems is influenced by various factors, conditions and processes. Among others, processes allowing organisms to deal with various types of stress are of key importance. In particular, oxidative stress as the result of the generation of reactive oxygen species (ROS) at the mitochondrial respiratory chain and the accumulation of ROS-induced molecular damage has been strongly linked to aging. Here we view the impact of ROS from a different angle: their role in the control of gene expression. We report a genome-wide transcriptome analysis of the fungal aging model Podospora anserina grown on medium containing paraquat (PQ). This treatment leads to an increased cellular generation and release of H2O2, a reduced growth rate, and a decrease in lifespan. The combined challenge by PQ and copper has a synergistic negative effect on growth and lifespan. The data from the transcriptome analysis of the wild type cultivated under PQ-stress and their comparison to those of a longitudinal aging study as well as of a copper-uptake longevity mutant of P. anserina revealed that PQ-stress leads to the up-regulation of transcripts coding for components involved in mitochondrial remodeling. PQ also affects the expression of copper-regulated genes suggesting an increase of cytoplasmic copper levels as it has been demonstrated earlier to occur during aging of P. anserina and during senescence of human fibroblasts. This effect may result from the induction of the mitochondrial permeability transition pore via PQ-induced ROS, leading to programmed cell death as part of an evolutionary conserved mechanism involved in biological aging and lifespan control.

show abstract

Section: Resultsmentioning

confidence: 99%

Effect of paraquat-induced oxidative stress on gene expression and aging of the filamentous ascomycete Podospora anserina

Wiemer¹,

Osiewacz

2014

MIC

View full text Add to dashboard Cite

show abstract

“…One of the bestcharacterized aging models with a clear mitochondrial etiology of aging is Podospora anserina. In this filamentous fungus, various mitochondrial pathways, including mtDNA reorganization, 23 mtDNA repair, 24 respiration, 25 ROS scavenging, 26,27 copper metabolism, 28 mitochondrial dynamics 29 and apoptosis, [30][31][32] have been demonstrated to play a role. Recently, an impact of the protein quality control system on aging has been demonstrated.…”

Section: Resultsmentioning

confidence: 99%

Unmasking a temperature-dependent effect of theP. anserinai-AAA protease on aging and development

Weil

Luce²,

Dröse³

et al. 2011

Cell Cycle

Self Cite

View full text Add to dashboard Cite

“…Catalases are enzymes responsible for the degradation of hydrogen peroxide (H 2 O 2 ) and protection of the cell against oxidative stress and reactive oxygen species (ROS) accumulation [ 45 , 46 ]. Acriflavine inhibits catalase activity in vitro in a competitive manner, which correlates with the conformational alterations of the enzyme structure caused by the inhibitory effect as previously observed by fluorescence spectroscopy [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

RNA-sequencing analysis of Trichophyton rubrumtranscriptome in response to sublethal doses of acriflavine

et al. 2014

View full text Add to dashboard Cite

Background The dermatophyte Trichophyton rubrum is an anthropophilic filamentous fungus that infects keratinized tissues and is the most common etiologic agent isolated in human dermatophytoses. The clinical treatment of these infections is challenging because only few antifungal drugs are commercially available. To understand the mode of action of cytotoxic drugs against fungi, we evaluated the time-dependent effects of acriflavine on T. rubrum transcriptome using high-throughput RNA-sequencing (RNA-seq) technology. Results RNA-seq analysis generated approximately 200 million short reads that were mapped to the Broad Institute's Dermatophyte Comparative Database before differential gene expression analysis was performed. By employing a stringent cut-off threshold of −1.5 and 1.5 log 2 -fold changes in gene expression, a subset of 490 unique genes were found to be modulated in T. rubrum in response to acriflavine exposure. Among the selected genes, 69 genes were modulated at all exposure time points. Functional categorization indicated the putative involvement of these genes in various cellular processes such as oxidation-reduction reaction, transmembrane transport, and metal ion binding. Interestingly, genes putatively involved in the pathogenicity of dermatophytoses were down-regulated suggesting that this drug interferes with the virulence of T. rubrum . Moreover, we identified 159 novel putative transcripts in intergenic regions and two transcripts in intron regions of T. rubrum genome. Conclusion The results provide insights into the molecular events underlying the stress responses of T. rubrum to acriflavine, revealing that this drug interfered with important molecular events involved in the establishment and maintenance of fungal infection in the host. In addition, the identification of novel transcripts will further enable the improvement of gene annotation and open reading frame prediction of T. rubrum and other dermatophyte genomes.

show abstract

PaCATB, a secreted catalase protecting Podospora anserina against exogenous oxidative stress

Cited by 16 publications

References 64 publications

Effect of paraquat-induced oxidative stress on gene expression and aging of the filamentous ascomycete Podospora anserina

Effect of paraquat-induced oxidative stress on gene expression and aging of the filamentous ascomycete Podospora anserina

Unmasking a temperature-dependent effect of theP. anserinai-AAA protease on aging and development

RNA-sequencing analysis of Trichophyton rubrumtranscriptome in response to sublethal doses of acriflavine

Contact Info

Product

Resources

About