p8 Deficiency Causes Siderosis in Spleens and Lymphocyte Apoptosis in Acute Pancreatitis

Weis, Sebastian; Schlaich, Tilmann; Dehghani, Faramarz; Carvalho, Tânia; Sommerer, Ines; Stephan, Frank; Kahlenberg, Franka; Mössner, Joachim; Hoffmeister, Albrecht

doi:10.1097/mpa.0000000000000172

Cited by 6 publications

(6 citation statements)

References 42 publications

(50 reference statements)

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“…However, CD3 + cells were not protected from apoptosis induction when NUPR1 was absent. Although our results do not imply an antiapoptotic role of NUPR1, Weis et al [11] suggested that NUPR1 functions as a blocker of CD3 + lymphocyte apoptosis since in both physiological conditions and after induction of inflammation, NUPR1 deletion coincides with increased CD3 + apoptosis. Due to experimental restrictions we were not able to identify the possible immune cell population in the lymph node that requires NUPR1 expression for initiation of the apoptotic process, but it can be speculated that these might be B lymphocytes that represent the second predominant population in cervical lymph nodes (around 30%).…”

Section: Discussioncontrasting

confidence: 90%

“…Also, NUPR1 negatively regulates the proliferation of myocytes and promotes myogenic differentiation [9,10]. As regards immune cells, it was shown that the number of all immune cells is comparable between NUPR1 -/and NUPR1 +/+ , though the apoptosis rate under normal and inflammatory conditions is increased in the absence of NUPR1, especially in CD3 + cells [11].…”

Section: Introductionmentioning

confidence: 99%

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The role of NUPR1 in lymphocyte proliferation and apoptosis

Vujičić¹,

Vasic²,

Nikolić³

et al. 2017

ARCH BIOL SCI BELGRA

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Nuclear protein 1 (NUPR1) is a transcription cofactor that senses stressful conditions and modulates cellular response by promoting or inhibiting apoptosis. NUPR1 is usually highly expressed in tumor cells where it enables them to adapt and resist environmental stress or chemotherapeutic compounds. NUPR1 can be involved in cell proliferation. Data about the involvement of NUPR1 in the proliferation and apoptosis of lymphocytes are scarce. Therefore, in this study we focused on the role of NUPR1 in lymphocyte physiology and found that NUPR1 might be involved in the initiation of their proliferation. Lymphocytes were isolated from the cervical lymph nodes of C57BL/6 mice. NUPR1 expression subsided 24 h after the induction of proliferation by a mitogen. Also, stressful conditions after cell isolation led to increased NUPR1 mRNA and protein expression in vitro that coincided with cell apoptosis. Similarly, apoptosis induction by staurosporine, a broadrange protein kinase inhibitor, led to increased NUPR1 expression. In addition, NUPR1 inhibition by small-interfering RNA prevented the staurosporine-induced apoptosis (judging from decreased caspase activity) in the whole cell population of cervical lymph nodes. However, NUPR1 absence was irrelevant to the induction of apoptosis in CD3 + T lymphocytes, suggesting that NUPR1 is probably a mediator of apoptosis in other immune cell populations within the lymph node, such as B lymphocytes. In conclusion, our results suggest that NUPR1 is important for the initiation of lymphocyte cell division and for the apoptotic process of non-T cells during stressful conditions.

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Section: Discussioncontrasting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

The role of NUPR1 in lymphocyte proliferation and apoptosis

Vujičić¹,

Vasic²,

Nikolić³

et al. 2017

ARCH BIOL SCI BELGRA

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“…Kataoka et al (2004) showed how important to study role of apoptosis in severe acute pancreatitis and how hard to make a clinical study in this field [13]. The obtained data about the correlations between severity of acute pancreatitis and lymphocytes apoptosis intensity in peripheral blood coheres with multiple findings in this field, such as Qin et al [14] findings and experimental findings by Pinhu et al [15], and Weis et al [16].…”

Section: Discussionsupporting

confidence: 59%

Development of lymphocytes apoptosis at the first phase of acute pancreatitis

Svetlana¹,

Antufrieva²

2018

Russ Open Med J

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Introduction -Acute pancreatitis takes the main place in the structure of surgical abdominal diseases. Endothelial dysfunction is one of the development mechanisms of the severe acute pancreatitis, which is accompanied by the formation of leucocytal and platelet microparticles, and massive cells apoptosis. Aim -To study lymphocytes apoptosis depending on severity of acute pancreatitis and area of pancreas and retroperitoneal cellular tissue affection. Material and Methods -We examined 40 patients with acute pancreatitis of IA phase of the disease, who were divided into two groups: with severe and non-severe course of the disease. Condition of lymphocytes plasma membrane in peripheral blood was estimated at admission of the patients by phase-contrast microscopy. Condition of pancreatic glandular tissue and retroperitoneal space were estimated by computed tomography angiography. Results -In most cases, non-severe acute pancreatitis was not associated with development of necrotic affection of pancreatic glandular tissue. In the event of severe acute pancreatitis structural changes of the pancreatic glandular tissue and retroperitoneal cellular tissue were in 95% of the cases. In cases of severe acute pancreatitis, the total blebbing of lymphocytes in terms of 100 cells was 33.5 (27.1, 39.7), terminal -21.9 (14.6, 28.3) (data presented as median with lower and upper quartiles). There were high positive correlations between the number of lymphocytes at terminal blebbing and affection in pancreatic gland tissue (r=0.76, p<0.001), also terminal blebbing and retroperitoneal space affection (r=0.82, p<0.001). Conclusion -Lymphocytes apoptosis in peripheral blood reflects the severity of acute pancreatitis.

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“…Samples from all animals were cut (5μm; Microm HM355S, Thermo Scientific, Walldorf, Germany), mounted and dried overnight at 45°C. Sections were stained with hematoxylin and eosin (HE) using a full-automatic microscope slide appliance (Shandon Varistain 24–4, Thermo Shandon Ltd.) as described [30]. Immunohistochemistry was performed as previously described [31].…”

Section: Methodsmentioning

confidence: 99%

Azithromycin does not improve disease severity in acute experimental pancreatitis

et al. 2019

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Acute pancreatitis is a severe systemic disease triggered by a sterile inflammation and initial local tissue damage of the pancreas. Immune cells infiltrating into the pancreas are main mediators of acute pancreatitis pathogenesis. In addition to their antimicrobial potency, macrolides possess anti-inflammatory and immunomodulatory properties which are routinely used in patients with chronic airway infections and might also beneficial in the treatment of acute lung injury. We here tested the hypothesis that the macrolide antibiotic azithromycin can improve the course of acute experimental pancreatitis via ameliorating the damage imposed by sterile inflammation, and could be used as a disease specific therapy. However, our data show that azithromycin does not have influence on caerulein induced acute pancreatitis in terms of reduction of organ damage, and disease severity. Furthermore Infiltration of immune cells into the pancreas or the lungs was not attenuated by azithromycin as compared to controls or ampicillin treated animals with acute experimental pancreatitis. We conclude that in the chosen model, azithromycin does not have any beneficial effects and that its immunomodulatory properties cannot be used to decrease disease severity in the model of caerulein-induced pancreatitis in mice.

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p8 Deficiency Causes Siderosis in Spleens and Lymphocyte Apoptosis in Acute Pancreatitis

Abstract: We conclude that p8 has no impact on the cellular composition of the adaptive and innate immune systems in noninflammatory conditions. However, it may limit apoptosis and maintain homeostasis of the immune reaction during AP.

Cited by 6 publications

References 42 publications

The role of NUPR1 in lymphocyte proliferation and apoptosis

The role of NUPR1 in lymphocyte proliferation and apoptosis

Development of lymphocytes apoptosis at the first phase of acute pancreatitis

Azithromycin does not improve disease severity in acute experimental pancreatitis

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