P78. The stro-1 + cell fraction of adult human bone marrow contains the osteogenic precursors

Gronthos, Stan; Graves, Stephen; Simmons, P. J.

doi:10.1016/8756-3282(94)90811-7

Cited by 185 publications

(247 citation statements)

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“…STRO-1 antigen is a differentiation antigen that is present on human bone marrow fibroblast-like cells and on various nonhaematopoietic progenitor cells. 16,17 In independent studies, a novel marker of human haematopoietic stem and progenitor cells was identified, characterized, and referred to as AC133 antigen. 6,7 The majority of cells reactive to the AC133 antibodies were found to co-express the human stem and progenitor marker CD34 and c-Kit.…”

Section: Discussionmentioning

confidence: 99%

Involvement of bone marrow-derived stem and progenitor cells in the pathogenesis of pterygium

Song

Kang

et al. 2004

Eye

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Aims To evaluate the involvement of multipotential stem and progenitor cells in the pathogenesis of pterygium. Methods Paraffin-embedded and snap-frozen primary pterygium (n ¼ 10) were serially sectioned and analysed immunohistochemically to determine the expression level of AC133 (marker for the primitive haematopoietic progenitors), CD34 (marker for the haematopoietic progenitor cells and endothelium), c-Kit (marker for haematopoietic and stromal progenitor cells), and STRO-1 (a differentiation antigen present on bone marrow fibroblast cells and on various nonhaematopoietic progenitor cells). Results In all the primary pterygium, immunoreactivity of AC133 and STRO-1 was found in some of the epithelial and stromal cells, CD34 was observed in the vascular endothelium, and some scattered ovoidal cells were found in the subepithelial connective tissue. C-Kit was expressed mainly in the basal epithelium of the head portions, and some spindle-shaped stromal cells. There is no immunoreactivity of AC133, c-Kit, and STRO-1 in normal conjunctiva, whereas CD34 was mildly stained with vessel wall. Conclusion Multipotential stem and progenitor cells may be involved in the pathogenesis of pterygium through its differentiation into fibroblasts and vascular endothelial cells.

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Section: Discussionmentioning

confidence: 99%

Involvement of bone marrow-derived stem and progenitor cells in the pathogenesis of pterygium

Song

Kang

et al. 2004

Eye

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“…Studies have explored the use of periosteal explants [28,29,37] or isolated cells [6,11,22] to generate bone [16,20] or cartilage [10,23,28,37,38]. Factors influencing cell phenotype include sex steroids [41,43], endogenous growth factors [26,45] and mechanical loading environment in vivo [27,42], and isolation [30], culture methods [8,13,20,31,37] and the donor site [14] in vitro.…”

Section: Introductionmentioning

confidence: 99%

Human Periosteum Is a Source of Cells for Orthopaedic Tissue Engineering: A Pilot Study

Ball

Bonzani

Bovis

et al. 2011

Clinical Orthopaedics &Amp; Related Research

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Background Periosteal cells are important in embryogenesis, fracture healing, and cartilage repair and could provide cells for osteochondral tissue engineering. Questions/purpose We determined whether a population of cells isolated from human periosteal tissue contains cells with a mesenchymal stem cell (MSC) phenotype and whether these cells can be expanded in culture and used to form tissue in vitro. Methods We obtained periosteal tissue from six patients. Initial expression of cell surface markers was assessed using flow cytometry. Cells were cultured over 10 generations and changes in gene expression evaluated to assess phenotypic stability. Phenotype was confirmed using flow cytometry and colony-forming ability assays. Mineral formation was assessed by culturing Stro-1 À and unsorted cells with osteogenic supplements. Three cell culture samples were used for a reverse transcription-polymerase chain reaction, four for flow cytometry, three for colony-forming assay, and three for mineralization. Results Primary cultures, containing large numbers of hematopoietic cells were replaced initially by Stro-1 and ALP-expressing immature osteoblastic cell types and later by ALP-expressing cells, which lacked Stro-1 and which became the predominant cell population during subculture.

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“…STRO-1 was originally reported to identify colony-forming osteogenic precursor cells isolated from bone marrow (28) and has been shown to have some promise for playing a role in immunophenotyping of MSCs (29). However, STRO-1 antigen is progressively lost in culture (30).…”

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confidence: 99%

Comparison of human stem cells derived from various mesenchymal tissues: Superiority of synovium as a cell source

Sakaguchi¹,

Sekiya²,

Yagishita³

et al. 2005

Arthritis & Rheumatism

1,351

1,170

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Objective. To compare the properties of human mesenchymal stem cells (MSCs) isolated from bone marrow, synovium, periosteum, skeletal muscle, and adipose tissue.Methods. Human mesenchymal tissues were obtained from 8 donors during knee surgery for ligament injury. After collagenase digestion or gradient-density separation, nucleated cells were plated at an appropriate density for expansion at the maximum rate without colony-to-colony contact. Yield, expandability, differentiation potential, and epitope profile were compared among MSCs from the 5 different tissue sources.Results. Colony number per 10 3 nucleated cells was lower, and cell number per colony was higher, in bone marrow than in other mesenchymal tissues. When the cells were replated at low density every 14 days, bone marrow-, synovium-, and periosteum-derived cells retained their proliferation ability even at passage 10. In chondrogenesis studies in which the cells were pelleted and cultured in vitro, pellets from bone marrow-, synovium-, and periosteum-derived cells were shown to be larger and stained more extensively for cartilage matrix. Synovium-derived cells, in particular, had the greatest ability for chondrogenesis. In adipogenesis experiments, the frequency of oil red O-positive colonies was highest in synovium-and adipose tissue-derived cells. In studies of osteogenesis, the rate of alizarin red-positive colonies was highest in bone marrow-, synovium-, and periosteum-derived cells. For epitope profiling, 15 surface antigens were measured. Most appeared to have similar epitope profiles irrespective of cell source.Conclusion. Our findings indicate that there are significant differences in MSC properties according to tissue source, beyond donor and experimental variation. Superiority of synovium as a potential source of MSCs for clinical applications was demonstrated.

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P78. The stro-1 + cell fraction of adult human bone marrow contains the osteogenic precursors

Cited by 185 publications

References 0 publications

Involvement of bone marrow-derived stem and progenitor cells in the pathogenesis of pterygium

Involvement of bone marrow-derived stem and progenitor cells in the pathogenesis of pterygium

Human Periosteum Is a Source of Cells for Orthopaedic Tissue Engineering: A Pilot Study

Comparison of human stem cells derived from various mesenchymal tissues: Superiority of synovium as a cell source

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