p73-regulated FER1L4 lncRNA sponges the oncogenic potential of miR-1273g-3p and aids in the suppression of colorectal cancer metastasis

Uboveja, Apoorva; Satija, Yatendra Kumar; Siraj, Fouzia; Saluja, Daman

doi:10.1016/j.isci.2022.103811

Cited by 9 publications

(5 citation statements)

References 57 publications

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“…2) A long non-coding RNA, FER1L4 , expression was higher in patients with SVs (Fig. 2B , upper middle panel), consistent with the fact that FER1L4 is the direct transcriptional target of TP73 [ 9 ]. In contrast, expressions of 3) DAB2 [ 10 ], 4) ARL11 [ 11 ], 5) PTK6 [ 12 ], and 6) TCAE3 [ 13 ], were lower in patients with SVs (Fig.…”

Section: To the Editorsupporting

confidence: 62%

Clinical landscape of TP73 structural variants in ATL patients

Hiramatsu,

Yokomori,

Shengyi

et al. 2023

Leukemia

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Section: To the Editorsupporting

confidence: 62%

Clinical landscape of TP73 structural variants in ATL patients

Hiramatsu,

Yokomori,

Shengyi

et al. 2023

Leukemia

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“…Since both DHD and valerosidate exhibited cell viability and anti-migratory effects on HCT116 cells, their regulatory effects on cancer-related proteins expressions were further explored. P53 and PTEN are crucial tumor suppressors in colorectal cancer (Uboveja et al, 2022). In other words, the loss of p53 or PTEN contributes to aggressive tumor growth and migration (Liebl and Hofmann, 2021;Serebriiskii et al, 2022).…”

Section: Resultsmentioning

confidence: 99%

Discovery of a heat-generated compound DHD derived from Patrinia villosa water extract with inhibitory effects on colon cancer cells viability and migration

Yang

Zheng

et al. 2023

Front. Chem.

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Introduction: The plant Patrinia villosa Juss. (PV) has long been used as a medicinal herb for treating intestinal disorders. Pharmacological activities such as anti-oxidation, anti-inflammation, and anti-cancer effects of compounds isolated from PV have been reported, but these bioactive compounds were not derived from PV water extract (PVW). Therefore, in the present study, we aimed to identify the active component(s) of PVW which exhibit inhibitory activities in colon cancer cells viability and migration.Methods: Human colon cancer HCT116 cells were treated with the isolated compounds of PVW and then subjected to MTT and transwell migration assays.Results: Our results showed that an active compound in PVW, 8,9-didehydro-7-hydroxydolichodial (DHD) inhibited cell viability of HCT116 cells, with IC50 value at 6.1 ± 2.2 μM. Interestingly, DHD was not detected in the herbal material of PV. Further investigation revealed that DHD is in fact a heat-generated compound derived from a natural compound present in PV, namely valerosidate. Valerosidate also reduced cell viability in HCT116 cells, with IC50 value at 22.2 ± 1.1 μM. Moreover, both DHD (2.75 μM) and valerosidate (10.81 μM) suppressed cell migration in HCT116 cells, with inhibitory rates at 74.8% and 74.6%, respectively. In addition, western blot results showed that DHD (5.5 μM) could significantly increase p53 expression by 34.8% and PTEN expression by 13.9%, while valerosidate (21.6 μM) could increase expressions of p53 and PTEN by 26.1% and 34.6%, respectively in HCT116 cells after 48 h treatment.Discussion: Taken together, this is the first report that a naturally-occurring valerosidate present in PV could actually transform to DHD by thermal hydrolysis, and both compounds exhibited inhibitory effects on cell viability and migration in HCT116 cells via increasing the expressions of tumor suppressors (p53 and PTEN). Our findings demonstrated that valerosidate is present in raw herb PV but not in PVW, while DHD is present in PVW rather than in raw herb PV. This difference in chemical profiles of raw herb and boiled water extract of PV may affect the anti-cancer activity, and hence further investigations are warranted.

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“…For the dual luciferase reporter assay, ODMECs were co-transfected with widetype (WT)-RASA1-UTR or mutant (Mut)-RASA1-UTR, pRL-TK, and miRNA mimic. The dual luciferase activity was measured after transfection as previously described [ 16 ].…”

Section: Methodsmentioning

confidence: 99%