P64. Evaluation of a modified nail cuticle bleeding model in rabbits and of the effect of active site inhibited factor VII (FVIIai) and heparin on bleeding in this model

Kristensen, Annemarie T.; Ezban, Mirella; Villumsen, A.; Andreassen, A. H.; Bregeng, C.; Hedner, Ulla

doi:10.1097/00001721-199604000-00123

Cited by 1 publication

(3 citation statements)

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“…No significant haemorrhagic side effects were found after application of FFR-rFVIIa in rabbits [10][11][12][13] or baboons, 23 indicating a species difference in sensitivity to FFR-rFVIIa. That rats appear to be more sensitive to FFR-rFVIIa is also supported by a study of gastric mucosa bleeding in rats, in which systemic application of FFR-rFVIIa resulted in a dose-dependent increase in blood loss.…”

Section: Discussionmentioning

confidence: 92%

“…2,18 In an attempt to reduce the incidence of vascular thrombosis, a variety of agents have been used in either experimental 1,[3][4][5][6][10][11][12][13]16 or clinical microvascular surgery. 2 Salemark 2 listed the most frequently used antithrombotic agents: preoperatively, aspirin was used most often; intraoperatively, heparin and dex- tran were widely used; and postoperatively, aspirin or dextran (separately or in combination) were the most common agents.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, intravenous administration of FFR-rFVIIa in a rabbit nail cuticle bleeding model did not result in significantly increased bleeding, indicating that FFR-rFVIIa is a safe antithrombotic drug in experimental rabbit models. 13 In our laboratory, we are working with an in vivo microvascular arterial thrombosis model in rats. 3,4,[14][15][16] Thrombosis is induced by performing a thrombogenic lesion in the femoral artery.…”

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confidence: 99%

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Local application of FFR-rFVIIa reduces thrombus formation at arterial anastomosis in rats

et al. 1999

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Thrombosis is still a significant problem in microvascular surgery. The aim of this study was to evaluate the antithrombotic effect of topically applied active site-inhibited recombinant human factor VIIa (FFR-rFVIIa) in a rat model with microvascular thrombosis. Forty-five male rats were allocated to one of three groups: local treatment with vehicle only, local treatment with 0.035 mg of FFR-rFVIIa, or local treatment with 0.35 mg of FFR-rFVIIa. An arteriotomy was made in the right femoral artery. Ten minutes following topical application, a thrombogenic anastomosis was performed. Using a transilluminator, thrombus formation and anastomotic bleeding episodes were observed and registered for 40 min. Local application of FFR-rFVIIa resulted in a 85-90% reduction of thrombus formation in both treated groups compared to the control group, but the reduction was only statistically significant in the group treated with 0.035 mg of FFR-rFVIIa. An increased occurrence and duration of anastomotic bleeding episodes were observed in both FFR-rFVIIa-treated groups.

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