p63 and its isoforms as markers of rat oral mucosa epidermal stem cells <i>in vitro</i>

Tao, Qian; Qiao, Bin; Lv, Biao; Zheng, Chao; Chen, Zhifeng; Huang, Hongzhang

doi:10.1002/cbf.1612

Cited by 10 publications

(3 citation statements)

References 19 publications

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“…40 Along with other putative stem cell markers such as α 6 β 4 , oct3/4, CD44H, p75, ATP-binding cassette subfamily G member 2 and K5, the epidermal markers are indeed expressed by the oral epithelium (Table 1). 30,31,32,41,42,43,44,45 …”

Section: Methods Used To Identify and Characterize Stem Cellsmentioning

confidence: 99%

Oral epithelial stem cells in tissue maintenance and disease: the first steps in a long journey

Jones

Klein

2013

Int J Oral Sci

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The identification and characterization of stem cells is a major focus of developmental biology and regenerative medicine. The advent of genetic inducible fate mapping techniques has made it possible to precisely label specific cell populations and to follow their progeny over time. When combined with advanced mathematical and statistical methods, stem cell division dynamics can be studied in new and exciting ways. Despite advances in a number of tissues, relatively little attention has been paid to stem cells in the oral epithelium. This review will focus on current knowledge about adult oral epithelial stem cells, paradigms in other epithelial stem cell systems that could facilitate new discoveries in this area and the potential roles of epithelial stem cells in oral disease.

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Section: Methods Used To Identify and Characterize Stem Cellsmentioning

confidence: 99%

Oral epithelial stem cells in tissue maintenance and disease: the first steps in a long journey

Jones

Klein

2013

Int J Oral Sci

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“…We first investigated the expression of undifferentiated cell markers Bmi-1 29 and the transcription factor p63α 30 . Bmi-1, a protein required for maintenance of adult stem cells 31 , was detected in the cell nucleus of only a few cells (Fig.…”

Section: Resultsmentioning

confidence: 99%

Tissue Harvesting Site and Culture Medium Affect Attachment, Growth, and Phenotype of Ex Vivo Expanded Oral Mucosal Epithelial Cells

Islam

Eidet

Badian

et al. 2017

Sci Rep

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Transplantation of cultured oral mucosal epithelial cells (OMECs) is a promising treatment strategy for limbal stem cell deficiency. In order to improve the culture method, we investigated the effects of four culture media and tissue harvesting sites on explant attachment, growth, and phenotype of OMECs cultured from Sprague-Dawley rats. Neither choice of media or harvesting site impacted the ability of the explants to attach to the culture well. Dulbecco’s modified Eagle’s medium/Ham’s F12 (DMEM) and Roswell Park Memorial Institute 1640 medium (RPMI) supported the largest cellular outgrowth. Fold outgrowth was superior from LL explants compared to explants from the buccal mucosa (BM), HP, and transition zone of the lower lip (TZ) after six-day culture. Putative stem cell markers were detected in cultures grown in DMEM and RPMI. In DMEM, cells from TZ showed higher colony-forming efficiency than LL, BM, and HP. In contrast to RPMI, DMEM both expressed the putative stem cell marker Bmi-1 and yielded cell colonies. Our data suggest that OMECs from LL and TZ cultured in DMEM give rise to undifferentiated cells with high growth capacity, and hence are the most promising for treatment of limbal stem cell deficiency.

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“…Oral mucosal epithelial SCs are located in the basal layer of this specialized epithelium [41] and can be identified with LSC markers such as p75 [42], CK-15 [43] and ΔNp63α [44]. Nakamura [45] and Nishida [46] both demonstrated that oral mucosal epithelial cells possess the potential to differentiate into cells of a corneal phenotype.…”

Section: Stem Cells In Other Epitheliamentioning

confidence: 99%

Tissue-Regenerating, Vision-Restoring Corneal Epithelial Stem Cells

Echevarria

Girolamo

2010

Stem Cell Rev and Rep

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The cornea, the most anterior segment of the eye, provides us with exquisite vision. Unlike other vital tissues, it is poorly protected from the environment and is thus reliant on a self-renewal program to preserve integrity. This function is reserved for corneal epithelial stem cells located in the basal layer of the limbus, a narrow transition zone that segregates the peripheral cornea from the adjacent conjunctiva. Under physiological conditions, these cells replenish the corneal epithelium when mature or traumatized cells are lost. However, when the limbus is extensively damaged, stem cell activity is compromised, resulting in a condition known as limbal stem cell deficiency (LSCD). This disease is characterized by corneal neovascularization and persistent epithelial defects which impair vision. Over the past 20 years a myriad of treatment options have been developed for LSCD, most of which incorporate stem cell transplantation. Due to the disadvantages associated with the use of allogeneic and xenogeneic material, researchers are currently focusing on refining techniques involving autologous limbal tissue transplantation and are delving into the possibility that stem cells found in other organs can provide an alternative source of corneal epithelium. Determining where donor stem cells reside on the recipient's ocular surface and how long they remain viable will provide further insights into improving current therapeutic options for patients with LSCD.

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p63 and its isoforms as markers of rat oral mucosa epidermal stem cells in vitro

Cited by 10 publications

References 19 publications

Oral epithelial stem cells in tissue maintenance and disease: the first steps in a long journey

Oral epithelial stem cells in tissue maintenance and disease: the first steps in a long journey

Tissue Harvesting Site and Culture Medium Affect Attachment, Growth, and Phenotype of Ex Vivo Expanded Oral Mucosal Epithelial Cells

Tissue-Regenerating, Vision-Restoring Corneal Epithelial Stem Cells

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