p63 and Brg1 control developmentally regulated higher-order chromatin remodelling at the epidermal differentiation complex locus in epidermal progenitor cells

Mardaryev, Andrei N.; Gdula, Michal R.; Yarker, Joanne L.; Emelianov, Vladimir U.; Poterlowicz, Krzysztof; Sharov, Andrey A.; Sharova, Tatyana; Scarpa, Julie A.; Joffe, Boris; Solovei, Irina; Chambon, Pierre; Botchkarev, Vladimir A.; Fessing, Michael Y.

doi:10.1242/dev.115725

Cited by 25 publications

(29 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In keratinocytes, p63 cooperates with an ATP-dependent chromatin remodeling factor, BAF1, to make these regions accessible. It has also been shown that p63 directly regulates chromatin factors such as Satb1 and Brg1 that play roles in higher-order chromatin remodeling, covalent histone modifications, and nuclear assembly (Fessing et al, 2011;Mardaryev et al, 2014). These data suggest that p63 regulates epidermal cell fate determination and differentiation not only through direct target genes but also via modulating the chromatin landscape.…”

Section: Introductionmentioning

confidence: 92%

Mutant p63 Affects Epidermal Cell Identity through Rewiring the Enhancer Landscape

Tanis

Smits

et al. 2018

Cell Reports

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 92%

Mutant p63 Affects Epidermal Cell Identity through Rewiring the Enhancer Landscape

Tanis

Smits

et al. 2018

Cell Reports

View full text Add to dashboard Cite

show abstract

“…One example is the indirect regulation of chromatin modifications by p63 during epidermis development (Fessing et al, ). More recently, P63 and Brg1 were shown to act through the epidermal differentiation complex (Mardaryev et al, ). More studies like this will help us understand how genes are regulated in clusters or circuits rather than one at a time to alter cell behavior.…”

Section: Pursuing Understanding At the Genomic Level: Systems Biologymentioning

confidence: 99%

Deciphering principles of morphogenesis from temporal and spatial patterns on the integument

Lai

Figueroa

et al. 2015

Developmental Dynamics

View full text Add to dashboard Cite

How tissue patterns form in development and regeneration is a fundamental issue remaining to be fully understood. The integument often forms repetitive units in space (periodic patterning) and time (cyclic renewal), such as feathers and hairs. Integument patterns are visible and experimentally manipulatable, helping us reveal pattern formative processes. Variability is seen in regional phenotypic specificities and temporal cycling at different physiological stages. Here we show some cellular / molecular bases revealed by analyzing integument patterns. 1) Localized cellular activity (proliferation, rearrangement, apoptosis, differentiation) transforms prototypic organ primordia into specific shapes. Combinatorial positioning of different localized activity zones generates diverse and complex organ forms. 2) Competitive equilibrium between activators and inhibitors regulates stem cells through cyclic quiescence and activation. Dynamic interactions between stem cells and their adjacent niche regulate regenerative behavior, modulated by multi-layers of macro-environmental factors (dermis, body hormone status and external environment). Genomics studies may reveal how positional information of localized cellular activity is stored. In vivo skin imaging and lineage tracing unveils new insights into stem cell plasticity. Principles of self-assembly obtained from the integumentary organ model can be applied to help restore damaged patterns during regenerative wound healing and for tissue engineering to rebuild tissues.

show abstract

“…However, upon differentiation, binding of EZH2 to the epidermal differentiation complex (EDC) is reduced, and AP1 induces EDC gene expression. Additionally, several transcription factors (TFs), including GRHL3 (Hopkin et al, 2012), Blimp1 (Magnusdottir et al, 2007), KLF4 (Patel et al, 2006), and ZNF750 (Sen et al, 2012), and genomic remodelers, including SATB1 (Fessing et al, 2011) and BRG1 (Mardaryev et al, 2014), have been implicated in late differentiation.…”

Section: Introductionmentioning

confidence: 99%

EGR3 Is a Late Epidermal Differentiation Regulator that Establishes the Skin-Specific Gene Network

Kim

Son

Kim

et al. 2019

Journal of Investigative Dermatology

View full text Add to dashboard Cite

Late epidermal differentiation is a key step of skin barrier formation; however, the specific genetic factors that distinguish late differentiation from early differentiation remain unknown. Here, we demonstrated that EGR3 is highly expressed in the stratum granulosum, and that it contributes to late epidermal differentiation. However, its expression is lost under poorly differentiated conditions, such as parakeratosis-lesional skin. EGR3 mediated the regulation of genes located in the epidermal differentiation complex through activation of enhancers and induction of enhancer RNAs. We further identified 20 targets of EGR3 specific for late differentiation. Additionally, we discovered that EGR3-and EGR3-related genes exhibited high tissue specificity on the skin. Through weighted gene co-expression analysis, EGR3 was found to be related to the keratinocyte differentiation-related module as an important part of the skin-specific genetic network. These findings shed light on the transcriptional regulation of late epidermal differentiation, highlighting candidate targets for diseases related to disrupted differentiation.

show abstract

p63 and Brg1 control developmentally regulated higher-order chromatin remodelling at the epidermal differentiation complex locus in epidermal progenitor cells

Cited by 25 publications

References 0 publications

Mutant p63 Affects Epidermal Cell Identity through Rewiring the Enhancer Landscape

Mutant p63 Affects Epidermal Cell Identity through Rewiring the Enhancer Landscape

Deciphering principles of morphogenesis from temporal and spatial patterns on the integument

EGR3 Is a Late Epidermal Differentiation Regulator that Establishes the Skin-Specific Gene Network

Contact Info

Product

Resources

About