P590The NO-donor SNAP exerts cardioprotection against ischemia/reperfusion injury in human embryonic stem cell-derived cardiomyocytes

Pálóczi, János; Varga, ZV; Apáti, Ágota; Szebényi, Nelli; Sarkadi, Balázs; Madonna, R; Csont, Tamás; Ferdinándy, Péter; Görbe, Anikó

doi:10.1093/cvr/cvu098.20

Cited by 1 publication

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“…[ 49 ] As access to human heart biopsies is restricted to availability, cardiomyocytes differentiated from human‐induced‐pluripotent‐stem‐cell‐derived cardiomyocytes (hiPSC‐CMs) or human embryonic stem cells have been employed to study myocardial I/R injury in vitro. [ 57,80,81 ]…”

Section: Experimental Models Of Myocardial I/r Injurymentioning

confidence: 99%

“…[49] As access to human heart biopsies is restricted to availability, cardiomyocytes differentiated from human-induced-pluripotent-stem-cellderived cardiomyocytes (hiPSC-CMs) or human embryonic stem cells have been employed to study myocardial I/R injury in vitro. [57,80,81] These cells are generally grown on either i) uncoated tissue plastic or ii) laminin-, fibronectin-, gelatin-, or poly(ethylene glycol (PEG)ylated-fibrin-precoated tissue plastic. [82][83][84] More recently, homotypic 2D cultures that better mimic blood flow and contractile activity of the heart microenvironment have been developed as iii) microfluidic devices and heart-on-a-chip (discussed below).…”

Section: D Monolayer Culturesmentioning

confidence: 99%

“…[84,85] Benefits: 2D monolayer cultures of cardiac cells are simple to use and well-established I/R models. Cells are exposed to [73][74][75] Rodent cell line (HL-1 and H9c2) [73,[77][78][79]84] Human-iPSC-derived cardiomyocytes [80,81] (patientderived cell [83] ) Rodent-stem-cell-derived cardiac cells [76] Uncoated tissue plastic [81] Fibronectin-Gelatin-precoated surface [82,83] PEGylated fibrin-precoated tissue plastic [84] Previously [98,102,[153][154][155][156][157][158] Chicken embryo [100] Human iPSCs derived cardiomyocytes [103,104,154,[159][160][161][162] Collagen sponges [98] Matrigel [98,102] Fibrinogen/Matrigel [155] Micromolded gelatin [159] Chitosan-enhanced extracellular-matrix (ECM) hydrogels [160] Fibrin-based EHT agarose casting molds with solid silicone racks [103] Polydimethysiloxane (PDMS) racks [154] Velcro-covered rods [100] Prolonged viability and measurable contractile properties in vitro Maturation of neonatal and iPSC-derived cardiomyo...…”

Section: D Monolayer Culturesmentioning

confidence: 99%

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Considerations for the Bioengineering of Advanced Cardiac In Vitro Models of Myocardial Infarction

Sharma

Wang

Ming

et al. 2021

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Despite the latest advances in cardiovascular biology and medicine, myocardial infarction (MI) remains one of the major causes of deaths worldwide. While reperfusion of the myocardium is critical to limit the ischemic damage typical of a MI event, it causes detrimental morphological and functional changes known as “reperfusion injury.” This complex scenario is poorly represented in currently available models of ischemia/reperfusion injury, leading to a poor translation of findings from the bench to the bedside. However, more recent bioengineered in vitro models of the human heart represent more clinically relevant tools to prevent and treat MI in patients. These include 3D cultures of cardiac cells, the use of patient‐derived stem cells, and 3D bioprinting technology. This review aims at highlighting the major features typical of a heart attack while comparing current in vitro, ex vivo, and in vivo models. This information has the potential to further guide in developing novel advanced in vitro cardiac models of ischemia/reperfusion injury. It may pave the way for the generation of advanced pathophysiological cardiac models with the potential to develop personalized therapies.

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Section: Experimental Models Of Myocardial I/r Injurymentioning

confidence: 99%