p53 sequence polymorphisms in late-stage leukemic Mytilus edulis are homologous with M. trossulus p53

Vassilenko, Ekaterina; Baldwin, Susan A.

doi:10.1007/s00227-013-2227-3

Cited by 5 publications

(6 citation statements)

References 34 publications

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“…The total number of mussels and, in parentheses, the number of mussels from CH4 sample are given. Data from Vassilenko et al (2010Vassilenko et al ( , 2013 et al, 2002) built with the PopART software (Leigh & Bryant, 2015). The analysis was based on the 542 bp alignment (provided as Data S2) of 80 sequences from the previous MtrBTN studies (Hammel et al, 2022;Skazina et al, 2021Skazina et al, , 2023Yonemitsu et al, 2019), 307 publicly available M. trossulus sequences (Breton et al, 2006;Chung et al, 2019;de Waard et al, 2019;Laakkonen et al, 2021;Layton et al, 2014;Marko et al, 2010) and their hybrids of different generations.…”

Section: Discussionmentioning

confidence: 99%

“…For Mtr BTN‐confirmed mussels, the haemolymph and the foot tissue were genotyped in parallel, while for the other mussels only the haemolymph was genotyped. The genotypes of mussels from our collections were compared with the genotypes of healthy mussels and mussels at the late stage of disseminated neoplasia (‘late leukaemic’ following the authors' classification) from British Columbia (Vassilenko & Baldwin, 2013; Vassilenko et al, 2010). These authors studied two samples: M. trossulus from local populations and M. edulis native to Prince Edward Island (Northwest Atlantic) cultured in British Columbia for experimental purposes (Figure 2, pink boxes).…”

Section: Methodsmentioning

confidence: 99%

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Genetic features of bivalve transmissible neoplasia in blue mussels from the Kola Bay (Barents Sea) suggest a recent trans‐Arctic migration of the cancer lineages

Skazina,

Ponomartsev,

Maiorova

et al. 2023

Molecular Ecology

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Ecology and biogeography of bivalve transmissible neoplasia (BTN) are underexplored due to its recent discovery and a challenging diagnostics. Blue mussels harbour two evolutionary lineages of BTN, MtrBTN1 and MtrBTN2, both derived from Mytilus trossulus. MtrBTN1 has been found only in M. trossulus from North Pacific. MtrBTN2 parasitizes different Mytilus spp. worldwide. BTN in M. trossulus in the Atlantic sector has never been studied. We looked for BTN in mussels from the Barents Sea using flow cytometry of cells, qPCR with primers specific to cancer‐associated alleles and sequencing of mtDNA and nuclear loci. Both MtrBTN1 and MtrBTN2 were present in our material, though their prevalence was low (~0.4%). All cancers parasitized M. trossulus except one, MtrBTN1, which was found in a hybrid between M. trossulus and M. edulis. The mtDNA haplotypes found in both lineages were nearly identical to those known from the Northwest Pacific but not from elsewhere. Our results suggest that these two lineages may have arrived in the Barents Sea in recent decades with the maritime transport along the Northern Sea Route. A young evolutionary age of MtrBTN1 seems to indicate that it is an emerging disease in the process of niche expansion. Comparing the new and the published sequence data on tumour suppressor p53, we proved that the prevalence of BTN in mussels can reach epizootic levels. The finding of diverse recombinants between paternally and maternally inherited mtDNAs in somatic tissues of M. trossulus was an unexpected result of our study.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Genetic features of bivalve transmissible neoplasia in blue mussels from the Kola Bay (Barents Sea) suggest a recent trans‐Arctic migration of the cancer lineages

Skazina,

Ponomartsev,

Maiorova

et al. 2023

Molecular Ecology

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“…When interpreting cancer genotypes, it was assumed that the peaks from cancer alleles were more pronounced in the hemolymph than in the foot tissues (see Figure S1). The genotypes of mussels from our collections were compared with the hemolymph genotypes of healthy mussels and mussels at the late stage of DN from British Columbia (Vassilenko et al, 2010; Vassilenko & Baldwin, 2013). These authors studied two samples: M. trossulus from local populations and M. edulis native to Prince Edward Island (Northwest Atlantic) cultured in British Columbia for experimental purposes.…”

Section: Methodsmentioning

confidence: 99%

“…Total frequencies and, in parentheses, frequencies for mussels from CH4 sample are given. Data from Vassilenko et al (2010Vassilenko et al ( , 2013 2) 13 ( 5…”

Section: Author Contributionsmentioning

confidence: 99%

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Molecular diversity of bivalve transmissible neoplasia of blue mussels in the Kola Bay (Barents Sea) indicates a recent migration of the cancer lineages between the North Pacific and Northern Europe

Skazina

Ponomartsev

Maiorova

et al. 2023

Preprint

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Bivalve transmissible neoplasia (BTN) is a leukemia-like cancer "metastasizing" by transmission of living cancer cells between molluscs. Blue mussels harbor two evolutionary lineages of BTN,MtrBTN1 andMtrBTN2, both derived fromMytilus trossulus. WhileMtrBTN1 has been found only inM. trossulusin North Pacific,MtrBTN2 parasitizes different Mytilus species worldwide, particularly in Western Europe. No targeted studies of BTN in Northern European mussels (M. edulis,M. trossulus) have been made. We searched for BTN in mussels from the Kola Bay (Barents Sea) with the help of flow cytometry of the hemolymph, qPCR with primers specific to cancer-associated alleles and sequencing of mitochondrial and nuclear loci. The species of the mussel hosts was ascertained genetically. BothMtrBTN1 andMtrBTN2 were present in our material, though their prevalence was low (~0.4%). The only instance ofMtrBTN2 was found inM. trossulus.MtrBTN1 occurred inM. trossulusand in a hybrid betweenM. trossulusandM. edulis. This finding indicates thatMtrBTN1 may potentially infect the latter species. The mtDNA haplotypes found in both lineages were nearly identical to those known from the North Pacific, but not from elsewhere. Our results suggest that they arrived in the Kola Bay fairly recently, probably with the maritime transport along the Northern Sea Route, and that the invasion was independent of that in Western Europe. A relatively young evolutionary age ofMtrBTN1 seems to suggest that it is an emerging disease in the process of niche expansion.

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Neoplasia

Newton

Smolowitz

Reynolds

et al. 2021

Invertebrate Medicine

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p53 sequence polymorphisms in late-stage leukemic Mytilus edulis are homologous with M. trossulus p53

Cited by 5 publications

References 34 publications

Genetic features of bivalve transmissible neoplasia in blue mussels from the Kola Bay (Barents Sea) suggest a recent trans‐Arctic migration of the cancer lineages

Genetic features of bivalve transmissible neoplasia in blue mussels from the Kola Bay (Barents Sea) suggest a recent trans‐Arctic migration of the cancer lineages

Molecular diversity of bivalve transmissible neoplasia of blue mussels in the Kola Bay (Barents Sea) indicates a recent migration of the cancer lineages between the North Pacific and Northern Europe

Neoplasia

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