p53 is not necessary for nuclear translocation of GAPDH during NO-induced apoptosis

Kim, Jum-Ji; Lee, Mi-Young

doi:10.5483/bmbrep.2011.44.12.782

Cited by 4 publications

(3 citation statements)

References 28 publications

(31 reference statements)

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“…Several proteins, such as GOSPEL [17], AIRE [18], SIRT1 [19], Mitochondrial uncoupling protein 2 (UCP2) [20] and CIB1 [21] can promote or suppress the nuclear translocation of GAPDH in various cell types. However, the mechanism by which GAPDH activates the cell death pathway in the nucleus remains largely unknown, despite several studies have suggested the involvement of p53, a cellular tumor suppresser [22,23]. …”

Section: Introductionmentioning

confidence: 99%

Disruption of the nuclear p53-GAPDH complex protects against ischemia-induced neuronal damage

et al. 2014

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Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is conventionally considered a critical enzyme that involves in glycolysis for energy production. Recent previous studies have suggested that GAPDH is important in glutamate-induced neuronal excitotoxicity, while accumulated evidence also demonstrated that GAPDH nuclear translocation plays a critical role in cell death. However, the molecular mechanisms underlying this process remain largely unknown. In this study, we showed that GAPDH translocates to the nucleus in a Siah1-dependent manner upon glutamate stimulation. The nuclear GAPDH forms a protein complex with p53 and enhances p53 expression and phosphorylation. Disruption of the GAPDH-p53 interaction with an interfering peptide blocks glutamate-induced cell death and GAPDH-mediated up-regulation of p53 expression and phosphorylation. Furthermore, administration of the interfering peptide in vivo protects against ischemia-induced cell death in rats subjected to tMCAo. Our data suggest that the nuclear p53-GAPDH complex is important in regulating glutamate-mediated neuronal death and could serve as a potential therapeutic target for ischemic stroke treatment.

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Section: Introductionmentioning

confidence: 99%

Disruption of the nuclear p53-GAPDH complex protects against ischemia-induced neuronal damage

et al. 2014

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“…Although originally thought to be only involved in glycolysis it is now apparent that GAPDH can be post-translationally modified resulting in its movement to the nucleus and the control of transcription [24]. GAPDH is targeted by a range of reactive molecules including ROS, NO and H 2 S [21].…”

mentioning

confidence: 99%

Competition of Reactive Signals and Thiol Modifications of Proteins

Hancock¹,

Craig²,

Whiteman³

2017

J Cell Signal

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“…MTT cell viability assay : B16F10 cell viability was measured by 3-[4,5-dimethylthylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay (Kim and Lee, 2011). The cells were 4 cultured in 96-well plates at a density of 1×10 cells per well.…”

mentioning

confidence: 99%

In-vitro studies on antioxidant, anti-melanogenic and anti-wrinkle effects of medicinal plant Glycyrrhiza uralensis

Ryu¹,

Kang²,

Lee³

2017

JEB

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Aim: Methodology:Results: Interpretation:The aim of the present study was to investigate the antioxidant, anti-melanogenic and anti-wrinkle activity of Glycyrrhiza uralensis (G. uralensis) for cosmeceutical application.The free radical scavenging activity of 1,3-butylene glycol (1,3-BG) extract of G. uralensis was analyzed using 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC) assays. The anti-melanogenesis and anti-wrinkle effects of G. uralensis extract were also analyzed by tyrosinase, zymography and collagen assays.The EC of 1,3-BG extract of G. uralensis, determined by DPPH and ABTS assays, was 0.59% 50 and 1.17%, respectively.The ORAC value of the extract was 41.56 μM trolox equivalent. The G. uralensis extract inhibited in vitro mushroom tyrosinase activity with an IC of 0.6%, and it also reduced melanin 50 contents in mouse melanoma cells. In addition, the G. uralensis extract notably inhibited matrix metalloproteinase-2 activity via zymography. Approximately, 60% collagen production enhancement was observed upon treatment with the extract, suggesting its anti-wrinkle potential.The 1,3-BG extract of G. uralensis may be utilized as a cosmeceutical ingredient, offering anti-oxidant, brightening and anti-wrinkle effects.

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p53 is not necessary for nuclear translocation of GAPDH during NO-induced apoptosis

Cited by 4 publications

References 28 publications

Disruption of the nuclear p53-GAPDH complex protects against ischemia-induced neuronal damage

Disruption of the nuclear p53-GAPDH complex protects against ischemia-induced neuronal damage

Competition of Reactive Signals and Thiol Modifications of Proteins

In-vitro studies on antioxidant, anti-melanogenic and anti-wrinkle effects of medicinal plant Glycyrrhiza uralensis

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