p53 expression in carcinoma of the cervix.

Hunt, C R; Hale, R J; Buckley, C. H.; Hunt, Jay D.

doi:10.1136/jcp.49.12.971

Cited by 23 publications

(21 citation statements)

References 26 publications

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“…It is, therefore, more appropriate to use the semiquantitative measure (at 5%), and to disregard the significant results relating to survival for p53 as measured by a small number of nuclei stained (< 5%). When a semiquantitative measure (> 5%) was applied, the prognostic effect was not significant (P = 0.64), which is similar to other reports (Hunt et al, 1996). van Diest et al (1997) highlight the problems with scoring immunohistochemistry and suggest a protocol to reduce the variability between published papers.…”

Section: Discussionsupporting

confidence: 72%

Prognostic significance of the bcl-2 apoptotic family of proteins in primary and recurrent cervical cancer

Crawford

Caldwell²,

Iles³

et al. 1998

Br J Cancer

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bcl-2 is one of a family of genes that control the apoptotic threshold of a cell. bcl-2 protein and its anti-apoptotic homologue, mcl-1, with the pro-apoptotic protein, bax, are thought to function by forming homo-and heterotypic dimers that then control the progression to apoptosis. p53 is also involved as a down-regulator of bcl-2 and a promoter of bax. To determine the effect of these apoptotic mechanisms, we used immunohistochemistry to determine the prognostic significance of the expression of bcl-2, mcl-1, bax and p53 in primary and recurrent cervical cancer. Tissues from 46 patients with primary cervical cancer and 28 women with recurrent carcinoma were stained for bcl-2, mcl-1, bax and p53. Kaplan-Meier survival analysis was performed using the log-rank test for differences between groups. In the primary disease group, positive staining for bcl-2 was associated with a better 5-year survival (bcl-2 +ve, 84% vs bcl-2 -ve, 53%, P = 0.03). Positive staining for p53 was associated with a survival disadvantage (p53 +ve, 4-year survival 38% vs p53 -ve, 4-year survival 78%, P= 0.02). mcl-1 and bax staining were not useful as prognostic indicators in primary disease. No marker was prognostic in recurrent disease. Positive bcl-2 staining defines a group of patients with primary disease with a good prognosis. p53, an activator of the bax promoter, identifies a group with a worse outcome. In recurrent disease, none of the markers reflected prognosis.

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Section: Discussionsupporting

confidence: 72%

Prognostic significance of the bcl-2 apoptotic family of proteins in primary and recurrent cervical cancer

Crawford

Caldwell²,

Iles³

et al. 1998

Br J Cancer

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“…The results are different from Hunt et al's [4] study in which p53 positivity was present in 22.7 % of cases with squamous cell cancer (n = 22), in 14.3 % of cases with adenocarcinoma (n = 35), and 16 % of cases with adenosquamous carcinoma (n = 25). Helland et al's study [10] showed p53 positivity in 44 % of cases with adenocarcinoma (n = 9) and 57 % of cases with adenosquamous carcinoma (n = 7).…”

Section: P53 Expression and Histopathology Of Cervical Cancercontrasting

confidence: 76%

“…negative status have p53 gene positivity, which is not significant. Hunt et al's study [4] showed the corresponding figures as 33 and 36 %, respectively. There was no relation between p53 overexpression and prognosis in cervical cancer [4,8].…”

Section: P53 Expression and Histopathology Of Cervical Cancermentioning

confidence: 89%

“…Hunt et al's study [4] showed the corresponding figures as 33 and 36 %, respectively. There was no relation between p53 overexpression and prognosis in cervical cancer [4,8]. The present study shows lack of relationship between p53 overexpression and prognosis in the cervical cancer patients.…”

Section: P53 Expression and Histopathology Of Cervical Cancermentioning

confidence: 89%

“…These observations had the possibility of a predisposive effect due to the small sample size of this study. Hunt et al study [4] showed p53 gene product was overexpressed in 17.1 % (14/82) of all carcinomas and also in areas of cervical intraepithelial neoplasia grade III adjacent to invasive squamous carcinoma. Inactivation of wild-type p53 gene product represents the most common genetic alteration in human carcinogenesis, and its overexpression, as detected by immunohistochemistry, has been proposed to indicate a worsened prognosis in some malignancies [5,6].…”

Section: Observationsmentioning

confidence: 95%

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The Role of p53 Gene in Cervical Carcinogenesis

Garima

Pandey

et al. 2015

J Obstet Gynecol India

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Aim To investigate the role of p53 gene in cervical carcinogenesis. Materials and Methods A total 50 cases and controls were taken after setting exclusion criteria. Venous blood (3 ml) samples were collected in sterile EDTA sterile vials. Both punch biopsy of cervical growth in cases and biopsy from cervix after hysterectomy in controls were performed. Genomic DNA was extracted from tissue and blood using standard protocol of Miller et al. 1994 using chloroformphenol method. Gene was amplified using specific forward and reverse primers and p53 gene expressions were studied. The present study of p53 gene regulation analyzed the expression of 279-bp bands on 1.5 agarose gel. Observations Out of the total 50 samples of cases and controls, we were able to isolate DNA from 38 cases and 28 controls in blood and in 22 cases and 22 controls in tissue. In cases of carcinoma cervix, p53 expression is either downregulated or absent in 71.06 % of cases compared to 50 % of controls in blood and 72.73 % of cases compared to 59.09 % of controls in tissue, but these figures were not statistically significant (p = 0.67 and p = 0.167, respectively). p53 positivity rate was only in 27.78 % of squamous cell cancer and 50 % of adenocarcinoma. Three out of nine patients (33.3 %) with L.N. positive status have p53 gene positivity, whereas 23 % (3

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Genetic alterations during the progression of squamous cell carcinomas of the uterine cervix

Kersemaekers

Vijver

Kenter

et al. 1999

Genes Chromosom. Cancer

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Most cervical carcinomas appear to arise from cervical intraepithelial neoplasia (CIN) lesions. In addition to infection with high‐risk human papilloma viruses, which is indicative of an increased risk of progression, alterations of oncogenes and tumor suppressor genes play a role. Genetic studies of CIN lesions, primary cervical carcinoma, and metastases may shed light on the relative importance of various genetic alterations involved in the progression of CIN to invasive carcinoma. We examined tumor material from 10 patients with squamous cell carcinoma of the uterine cervix and synchronous CIN lesions and lymph node metastases. The CIN component, invasive carcinoma, and lymph node metastases were analyzed separately for loss of heterozygosity (LOH) on the following loci: VHL (3p21), HLA region (6p22–23), PGL (11q 22–24), E6 associated protein (15q11–13), TP53 (17p13), DCC (18q21.1), and chromosomes 1, 2, 4, 9, 20, and X. Using immunohistochemistry, the expression of the EGF receptor, ERBB2, and TP53 was determined. In CIN lesions, frequent LOH was found at chromosome arms 3p, 6p, and 11q. Primary invasive carcinoma showed additional LOH at chromosome arms 6q, 17p, and 18q. In lymph node metastases, an additional locus on the X chromosome displayed LOH. All carcinomas and synchronous lesions but one showed high expression levels of the EGF receptor. TP53 staining, when present, was found in all synchronous lesions. Focal staining of ERBB2 was found in one CIN lesion, two invasive carcinomas, and four metastases. The molecular alterations accumulated in a fashion that paralleled the progression of the tumors. These results indicate that cervical tumorigenesis occurs in a stepwise fashion, including infection and integration of oncogenic HPV and several specific genetic alterations. Genes Chromosomes Cancer 26:346–354, 1999. © 1999 Wiley‐Liss, Inc.

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p53 expression in carcinoma of the cervix.

Cited by 23 publications

References 26 publications

Prognostic significance of the bcl-2 apoptotic family of proteins in primary and recurrent cervical cancer

Prognostic significance of the bcl-2 apoptotic family of proteins in primary and recurrent cervical cancer

The Role of p53 Gene in Cervical Carcinogenesis

Genetic alterations during the progression of squamous cell carcinomas of the uterine cervix

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